An overview of current research and developments in biosurfactants

Vieira, Isabela Maria Monteiro; Santos, Brenda Lohanny Passos; Ruzene, Denise Santos; Silva, Daniel Pereira da

doi:10.1016/j.jiec.2021.05.017

Cited by 64 publications

(29 citation statements)

References 171 publications

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“…Biosurfactants produced by microorganisms are usually more biodegradable and eco-friendly and less irritating than synthetic surfactants [ 2 , 5 , 6 , 7 ]. The combination of biosurfactants and synthetic surfactants in the formulation can improve the skin gentleness of cleaning products.…”

Section: Introductionmentioning

confidence: 99%

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Ma²,

Chen³

et al. 2022

Molecules

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Objectives: Biosurfactants with anti-inflammatory activity may alleviate skin irritation caused by synthetic surfactants in cleaning products. Sophorolipid (SL) is a promising alternative to synthetic surfactants. However, there are few reports on the anti-inflammatory activity of SL and the underlying mechanism. The purpose of this work is to verify that lipopolysaccharide (LPS)-induced inflammation could be inhibited through targeting the pathway of nuclear factor-κB (NF-κB) in RAW264.7 cells. Methods: The influence of SL on cytokine release was investigated by LPS-induced RAW264.7 cells using ELISA. The quantification of the protein expression of corresponding molecular markers was realized by Western blot analysis. Flow cytometry was employed to determine the levels of Ca2+ and reactive oxygen species (ROS). The relative expression of inducible nitric oxide synthase (INOS) and cyclooxygenase-2 (COX-2) was determined by RT-PCR. An immunofluorescence assay and confocal microscope were used to observe the NF-κB/p65 translocation from the cytoplasm into the nucleus. The likely targets of SL were predicted by molecular docking analysis. Results: SL showed anti-inflammatory activity and reduced the release of inflammatory cytokines including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and nitric oxide (NO). The experimental results show that SL suppressed the Ca2+ and ROS levels influx in the LPS-induced RAW264.7 cells and alleviated the LPS-induced expression of iNOS and COX-2, the LPS-induced translocation of NF-κB (p65) from the cytoplasm into the nucleus, and the expression of phosphorylated proteins such as p65 and IκBα. Furthermore, molecular docking analysis showed that SL may inhibit inflammatory signaling by competing with LPS to bind TLR4/MD-2 through hydrophobic interactions and by inhibiting IKKβ activation through the hydrogen bonding and hydrophobic interactions. Conclusion: This study demonstrated that SL exerted anti-inflammatory activity via the pathway of NF-κB in RAW264.7 cells.

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Section: Introductionmentioning

confidence: 99%

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Ma²,

Chen³

et al. 2022

Molecules

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“…[1][2][3] The best-known biosurfactants are glycosylated lipids (sophorolipids, rhamnolipids, mannosylerythritole lipids…) and derivatives of small peptides (surfactin), and they have been developed for decades to replace synthetic surfactants for their lower environmental impact. [1][2][3] Biosurfactants are indeed considered to be more biodegradable and less toxic than petrochemical surfactants, and therefore they find use in a number of applications in detergency, cosmetics, environmental science, or as antimicrobial compounds, [4][5][6] with a milder effect on protein denaturation. 7 Nonetheless, the word biosurfactant is slightly reductive, as these molecules often present a richer self-assembly and phase behavior than classical surfactants.…”

Section: Introductionmentioning

confidence: 99%

“…) at 80 wt%,(3) at 70 wt%, and (4) at 70 wt%; polarized-light microscopy images of compounds (3) at 80 wt% and (4) at 70 wt%.ACKNOWLEDGMENTSClaire Goldmann (LPS, Université Paris-Saclay, CNRS) and Stéphan Rouzière (LPS-Plateforme Morpheus, Université Paris-Saclay, CNRS) are kindly acknowledged for, respectively, in-lab support and assistance with the X-ray scattering experiments.…”

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confidence: 99%

Lyotropic Liquid-Crystalline Phases of Sophorolipid Biosurfactants

et al. 2022

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“…Os surfactantes são moléculas anfifílicas compostas por uma porção polar e outra apolar (Figura 1 Os tensoativos desempenham papel essencial no aumento da biodisponibilidade de moléculas hidrofóbicas, poderem formar complexos com metais pesados, além de participarem em processos fisiológicos de sinalização e comunicação célula-célula, motilidade, diferenciação celular, adesão a substratos, proteção contra elementos tóxicos, formação de biofilmes, além de atuarem como moléculas de armazenamento de carbono e energia. 28 Os lipopeptídeos e lipoproteínas possuem um domínio hidrofílico formado por um peptídeo associado a um domínio lipídico e são moléculas amplamente produzidas pelo gênero Bacillus. As famílias ou subclasses mais comumente produzidas são surfactina/liquenisina, iturina, fengicina, kurstakina, daptomicina e viscosina.…”

Section: Biossurfactanteunclassified

“…e Serratia marcences. 28,[31][32] Emulsões são sistemas formados por duas fases líquidas imiscíveis. Ocorre quando uma fase interna, chamada de fase dispersa, se encontra no interior da outra fase, em formato de gotículas.…”

Section: Biossurfactanteunclassified

Biossurfactante produzido por linhagem termo-halofílica de >i<Bacillus alveayuensis>/i<: caracterização química e avaliação da atividade tensoativa em meio poroso com óleo e sobre microrganismos

Argentin¹

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foi usado em testes de atividade antifúngica contra onze linhagens de fungos fitopatogênicos, onde foi observada completa inibição do crescimento das hifas em Moniliophthora perniciosa, Phytophthora sojae e Lasidiplodia euphorbicola e inibição parcial em Ceratocystis paradoxa, Sclerotinia sclerotiorum, Rhizopus microsporus, Aspergillus flavus e Aspergillus niger. A atividade do tensoativo também foi testada contra as linhagens bacterianas Bacillus subtilis (ATTC 6633 e 21332), Staphylococcus epidermidis (ATTC 12228 e 35984), Pseudomonas fluorescens (ATTC 13525), Escherichia coli (ATTC 25922) e Desulfovibrio marinus BRS-1. Nos testes de microdiluição o biossurfactante causou uma inibição inicial pronunciada no crescimento de B. subtilis 6633, S. epidermidis (12228 e 35984), e moderada em P. fluorescens 13525. Sobre a bactéria redutora de sulfato D. marinus BRS-1 o biossurfactante mostrou ação bacteriostática e bactericida nas concentrações 2.250e 2.500 µg/mL, respectivamente. O tensoativo semipurificado teve sua capacidade de recuperação de óleo em meio poroso avaliada pelo método de lavagem de areia, sendo capaz de remover, aproximadamente, 30% do óleo de motor (10W40) presente na amostra. O bissurfactante produzido pela linhagem estudada mostrou potencial aplicação em processos de MEOR e biorremediação de óleo, além de ser capaz de inibir o crescimento de fungos e bactérias, podendo ter utilidade com agente de biocontrole.

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An overview of current research and developments in biosurfactants

Cited by 64 publications

References 171 publications

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Sophorolipid Suppresses LPS-Induced Inflammation in RAW264.7 Cells through the NF-κB Signaling Pathway

Lyotropic Liquid-Crystalline Phases of Sophorolipid Biosurfactants

Biossurfactante produzido por linhagem termo-halofílica de >i<Bacillus alveayuensis>/i<: caracterização química e avaliação da atividade tensoativa em meio poroso com óleo e sobre microrganismos

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