An overview of macrophage-fungal interactions

Ra, Fromtling; Hj, Shadomy

doi:10.1007/bf00437738

Cited by 43 publications

(18 citation statements)

References 122 publications

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“…Patients with reduced immunological capability due to disease or certain forms of chemotherapy are more susceptible to opportunistic fungal infection (10,12). When administered to neutropenic patients, drugs that inhibit the CL response may further reduce the already-impaired immune response of the host.…”

Section: Resultsmentioning

confidence: 99%

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells

Abruzzo¹,

Giltinan²,

Capizzi³

et al. 1986

Antimicrob Agents Chemother

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The luminol-enhanced chemiluminescence (CL) assay is a measure of the early events of phagocytosis, leukocyte activation, and immune cell interactions. Reduction in the CL response of immune cells may be indicative of an inhibition of the immune response. This study was undertaken to examine the effects of antifungal agents tested at concentrations above and below therapeutically achievable levels on the CL response of mouse spleen cells. The effects of six antifungal agents-amphotericin B, ketoconazole, miconazole, 5-fluorocytosine, Bay-n-7133, and ICI 153,066-were studied. Changes in the CL response were assessed in terms of peak intensity and area under the intensity-time curve compared with appropriate diluent controls for each drug. Only amphotericin B and ketoconazole caused statistically significant lowering of the peak intensity at levels that are therapeutically attainable (mean peak plasma levels of 2 to 4 and 3.5 to 16 ,ug/ml, respectively). Although amphotericin B and ketoconazole caused reduction in the CL response, removal of the unbound drug in the preparation by centrifugation, washing, and suspension of cells in fresh drug-free medium resulted in a return of CL activity equivalent to the activity observed in cells not exposed to these agents. These results suggest that amphotericin B and ketoconazole at therapeutic concentrations may cause a reduction in immune cell antimicrobial activity; the clinical significance of these observations remains to be determined.

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Section: Resultsmentioning

confidence: 99%

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells

Abruzzo¹,

Giltinan²,

Capizzi³

et al. 1986

Antimicrob Agents Chemother

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show abstract

“…These phagocytes play a key role in host defense by ingesting and inactivating invading organisms, cytokine production, and interacting with other adaptive immune cells such as T and B lymphocytes (13,15). Thus, monocytes play a pivotal role in immune regulation by producing TNF-α, IL-1, IL-6, IL-8, IL-10, IL-12, and TGF-β1 (36).…”

Section: Pcm (2)mentioning

confidence: 99%

Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

Kurokawa

Araújo

Soares

et al. 2007

Microbiology and Immunology

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Monocytes and macrophages play a central role in innate and adaptive immune response against systemic fungal infections. Imbalances in suppressor or stimulatory cytokine secretion caused by these cells may influence disease development, microorganism death, and the nature of the adaptive immune response. This study analyzed the monocyte cytokine profiles of healthy individuals challenged with high and low virulent strains of P. brasiliensis and mRNA cytokine expression kinetics by reverse transcription polymerase chain reaction (RT‐PCR). Peripheral blood monocytes from healthy volunteers were cultured in vitro with and without virulent (Pb18) or low virulence (Pb265) strains from P. brasiliensis viable yeast cells. Interleukin‐1 beta (IL‐1β), IL‐6, IL‐8, IL‐10, tumor necrosis factor‐alpha (TNF‐α), and transforming growth factor‐beta (TGF‐β1) were measured in culture supernatants by enzyme immunoassay (ELISA), and mRNA cytokine expression was determined by RT‐PCR at 0, 4, 8, 12, 18 and 48 hr. Both P. brasiliensis strains induced monocyte production of IL‐1β, IL‐6, IL‐10 and TNF‐α. Pb18 induced higher levels of IL‐1β, IL‐6, and IL‐10 than Pb265. IL‐8 and TGF‐β1 levels were not significantly different from those cultured without stimulus. The mRNA cytokine expression was similar to supernatant cytokines measured by ELISA. In vitro monocyte challenge with virulent P. brasiliensis strain induces earlier and higher levels of pro‐ and anti‐inflammatory cytokines than low virulence strain.

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“…Because of their phagocytic properties, PMN cells and macrophages represent a useful first line defence barrier against C. albicans infections, as documented by numerous studies on the C. albicans yeastform (Baccarini et al, 1983Bistoni et al, 1983;Decker et al, 1986;Djeu et al, 1986a;Fromtling & Shadomy, 1986;Djeu & Blanchard, 1987;Leijh et al, 1977 ;Blasi et al, 1990,199 1 ;Kolotila & Diamond, 1990 ;Vecchiarelli et al, 1991). However, the dimorphic transition from the yeast to the hyphal form, enables C. albicans to avoid phagocytic clearance and invade tissues, where virtually only hyphae are found (reviewed in Odds, 1988).…”

Section: Discussionmentioning

confidence: 99%

Fungicidal activity of Candida albicans-induced murine lymphokine-activated killer cells against C. albicans hyphae in vitro

Scaringi

Blasi

Rosati

et al. 1991

Journal of General Microbiology

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An overview of macrophage-fungal interactions

Cited by 43 publications

References 122 publications

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells

Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

Fungicidal activity of Candida albicans-induced murine lymphokine-activated killer cells against C. albicans hyphae in vitro

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