An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19

Kailasa, Suresh Kumar; Mehta, Vaibhavkumar N.; Koduru, Janardhan Reddy; Basu, Hirakendu; Singhal, R. K.; Murthy, Z.V.P.; Park, Tae Jung

doi:10.1039/d0an01528h

Cited by 47 publications

(28 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is also due to the fact that in contrast to other promising paper-based analytical platforms that mainly remain as laboratory prototypes, LFIA devices easily enter into real-life applications with a high market penetration mainly because they do not need extensive upgrades to become an end-user device [ 18 ]. The wide acceptance of LFIA devices also played an eminent role in the use of the platform for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in the current pandemic situation, as we can still notice and as extensively reported in the literature [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Nardo

Chiarello

Cavalera

et al. 2021

Sensors

256

162

View full text Add to dashboard Cite

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of molecules, organisms, and (bio)markers for clinical purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Nardo

Chiarello

Cavalera

et al. 2021

Sensors

256

162

View full text Add to dashboard Cite

show abstract

“… S. no. Method Read out time Sensitivity/Specificity References 1 RT-qPCR 4 h 71%/NA 29 , 115 2 RT-digital PCR 90%/100% 71 , 110 , 127 3 MNPs based RT-qPCR 30 min NA 43 , 141 4 LAMP-based colorimetric method 20–30 min 97.6% 107 , 136 5 CT Scan NA 97%/25% 2 , 87 , 108 6 LFICS-Au NPs colloid (IgM + IgG) 15 min 88.66%/90.63% 20 , 61 7 ELISA (IgM + IgG) 2 h 87.3%/100% 123 8 Chemiluminescence (total Ab)/Automated NA 86.9%/99.2% 51 , 122 9 CRISPR-based assay 30–40 min 95%/100% …”

Section: Current Biosensing Techniques Focused On Covid-19 Diagnosismentioning

confidence: 99%

Metal Organic Frameworks as Biosensing Materials for COVID-19

2021

View full text Add to dashboard Cite

The novel coronavirus disease (COVID-19) pandemic outbreak is the most startling public health crises with attendant global socio-economic burden ever experienced in the twenty-first century. The level of devastation by this outbreak is such that highly impacted countries will take years to recover. Studies have shown that timely detection based on accelerated sample testing and accurate diagnosis are crucial steps to reducing or preventing the spread of any pandemic outbreak. In this opinionated review, the impacts of metal organic frameworks (MOFs) as a biosensor in a pandemic outbreak is investigated with reference to COVID-19. Biosensing technologies have been proven to be very effective in clinical analyses, especially in assessment of severe infectious diseases. Polymerase chain reactions (PCR, RT-PCR, CRISPR) - based test methods predominantly used for SARS-COV-2 diagnoses have serious limitations and the health scientists and researchers are urged to come up with a more robust and versatile system for solving diagnostic problem associated with the current and future pandemic outbreaks. MOFs, an emerging crystalline material with unique characteristics will serve as promising biosensing materials in a pandemic outbreak such as the one we are in. We hereby highlight the characteristics of MOFs and their sensing applications, potentials as biosensors in a pandemic outbreak and draw the attention of researchers to a new vista of research that needs immediate action.

show abstract

“…The analytical sensitivity and versatility have made LFIA the most commercially available POC diagnostic platform (Nardo et al 2021; 2). The wide acceptance of LFIA platform has also played a prominent role in the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Espejo et al 2020;Ruhan et al 2020;Zhu and Woong 2020;Deeks et al 2020;Dinnes et al 2020;Vashist 2020;Mekonnen et al 2020;Kailasa et al 2021;Antiochia 2021;Ernst et al 2021) (Fig. 2).…”

Section: Lateral Flow Immunoassaymentioning

confidence: 99%

“…The advancement in LFIA technology is the development of multiplex systems or format which allows the detection of various bacterial or viral antigens in a single assay (Nardo et al 2021 ; Andryukov 2020 ; Jørgensen et al 2015 ) (Table 2 ). The wide acceptance of LFIA platform has also played a prominent role in the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (Espejo et al 2020 ; Ruhan et al 2020 ; Zhu and Woong 2020 ; Deeks et al 2020 ; Dinnes et al 2020 ; Vashist 2020 ; Mekonnen et al 2020 ; Kailasa et al 2021 ; Antiochia 2021 ; Ernst et al 2021 ) (Fig. 2 ).…”

Section: Introductionmentioning

confidence: 99%

Monoplex and multiplex immunoassays: approval, advancements, and alternatives

Ahsan

2021

Comp Clin Pathol

View full text Add to dashboard Cite

An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19

Abstract: Molecular biology and nanotechnology based analytical methods have proven to be promising diagnostic tools for the rapid diagnosis of COVID-19.

Cited by 47 publications

References 89 publications

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Metal Organic Frameworks as Biosensing Materials for COVID-19

Monoplex and multiplex immunoassays: approval, advancements, and alternatives

Contact Info

Product

Resources

About