Sugarcane (Saccharum officinarum L.) is one of the most important field crops grown in the tropics and sub-tropics. More than half of the world's sugar is derived from sugar cane. Conventional methods have greatly contributed to crop improvement; however limitations such as complex genome, narrow genetic base, poor fertility, susceptibility to biotic and abiotic stresses and long duration to breed elite cultivars still impose a challenge. Sugarcane, thus, is a suitable candidate for application of biotechnology and genetic engineering tools. In this direction, in vitro culture systems and related biotechnologies have been developed as novel strategies for sugarcane improvement. Studies have been conducted towards employing in vitro culture combined with radiation/chemical induced mutagenesis for mutant isolation. Advancements in genomics tools have paved the way for a detailed understanding of the mechanism underlying biotic and abiotic stress responses. The potential of the current genomics programs, aimed at elucidating the structure, function, and interactions of the sugarcane genes, will revolutionize the application of biotechnology to crop improvement. Genetically modified sugarcane with increased resistance to agronomic traits including biotic and abiotic stresses, yield and juice could become useful in breeding for better varieties. This review outlines some of the biotechnological developments that are in place and tailored to address important issues related to sugarcane improvement.