An overview of post transplantation events of decellularized scaffolds

Talaei‐Khozani, Tahereh; Yaghoubi, Atefeh

doi:10.1016/j.trim.2022.101640

Cited by 9 publications

(3 citation statements)

References 104 publications

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“…Ultimately, the findings of the present study complement the current research on animal dECM-derived biomaterials for wound healing and regenerative medicine applications, further proving the importance of exploiting ECM components to achieve bioactivity. Moreover, there are other advantages to using animal derived biomaterials, such as: (i) the low immunogenicity of the product, guaranteed by the decellularization process used, should reduce the risk of rejection after implant [ 36 , 41 ] and, (ii) being a large animal derived food industry by-product, it should be available in large quantities and easily obtainable when compared to cell derived or human sources of dECM [ 35 ]. However, there are still some disadvantages and risks in using these biomaterials.…”

Section: Discussionmentioning

confidence: 99%

“…Their biological advantage is mainly given by their complex structure and composition, which greatly conserves the native ECM, thereby maintaining the ability to support cellular behavior and tissue functions [ 34 , 35 ]. Moreover, these bioactive materials naturally possess both angiogenic and immunomodulatory potential [ 36 , 37 , 38 , 39 ]. This leads not only to a number of applications of decellularized ECM-derived materials in regenerative medicine [ 40 ], but also to the high interest in using them as multi-functional wound dressing [ 14 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

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Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications

Francesco

Bertani

Fusaro³

et al. 2022

Biomolecules

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Recent advancements in regenerative medicine have enhanced the development of biomaterials as multi-functional dressings, capable of accelerating wound healing and addressing the challenge of chronic wounds. Hydrogels obtained from decellularized tissues have a complex composition, comparable to the native extracellular environment, showing highly interesting characteristics for wound healing applications. In this study, a bovine pericardium decellularized extracellular matrix (dECM) hydrogel was characterized in terms of macromolecules content, and its immunomodulatory, angiogenic and wound healing potential has been evaluated. The polarization profile of human monocytes-derived macrophages seeded on dECM hydrogel was assessed by RT-qPCR. Angiogenic markers expression has been evaluated by Western blot and antibody array on cell lysates derived from endothelial cells cultured on dECM hydrogel, and a murine in vivo model of hindlimb ischemia was used to evaluate the angiogenic potential. Fibroblast migration was assessed by a transwell migration assay, and an in vivo murine wound healing model treated with dECM hydrogels was also used. The results showed a complex composition, of which the major component is collagen type I. The dECM hydrogel is biocompatible, able to drive M2 phenotype polarization, stimulate the expression of angiogenic markers in vitro, and prevent loss of functionality in hindlimb ischemia model. Furthermore, it drives fibroblast migration and shows ability to facilitate wound closure in vivo, demonstrating its great potential for regenerative applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications

Francesco

Bertani

Fusaro³

et al. 2022

Biomolecules

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“…Native cardiac ECM is a promising biomedical material that can be applied to reconstructive surgery, due to its structural and functional resemblance to native tissue [ 7 ]. Additionally, native cardiac ECM has been used as a scaffold for 3D culturing [ 8 , 9 ]. Previous research has shown that injectable biohybrid ECM-based hydrogels promote cardiac tissue regeneration [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

From Macro to Micro: Comparison of Imaging Techniques to Detect Vascular Network Formation in Left Ventricle Decellularized Extracellular Matrix Hydrogels

Zhang

Getova

Martínez-García

et al. 2022

Gels

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Background: Angiogenesis is a crucial process in physiological maintenance and tissue regeneration. To understand the contribution of angiogenesis, it is essential to replicate this process in an environment that reproduces the biochemical and physical properties which are largely governed by the extracellular matrix (ECM). We investigated vascularization in cardiac left ventricular ECM hydrogels to mimic post-myocardial repair. We set out to assess and compare different destructive and non-destructive methods, optical as well as non-optical, to visualize angiogenesis and associated matrix remodeling in myocardial ECM hydrogels. Methods: A total of 100,000, 300,000, and 600,000 Human Pulmonary Microvascular Endothelial Cells (HPMEC) were seeded in left ventricular cardiac ECM hydrogel in 48-well plates. After 1, 7, and 14 days of culture, the HPMEC were imaged by inverted fluorescence microscopy and 3D confocal laser scanning microscopy (Zeiss Cell Discoverer 7). In addition, cell-seeded ECM hydrogels were scanned by optical coherence tomography (OCT). Fixed and paraffin-embedded gels were thin-sectioned and assessed for ECM components via H&E, picrosirius red histochemical staining, and immunostaining for collagen type I. ImageJ-based densitometry was used to quantify vascular-like networks and GraphPad was used for statistical analyses. Results: Qualitative analyses were realized through fluoromicrographs obtained by the confocal laser scanning microscope which allowed us to visualize the extensive vascular-like networks that readily appeared at all seeding densities. Quantification of networks was only possible using fluoromicrographs from inverted microscopy. These showed that, after three days, the number of master junctions was seeding density-dependent. The resolution of optical coherence tomography was too low to distinguish between signals caused by the ECM and cells or networks, yet it did show that gels, irrespective of cells, were heterogeneous. Interestingly, (immuno)histochemistry could clearly distinguish between the cast cardiac-derived matrix and newly deposited ECM in the hydrogels. The H&E staining corroborated the presence of vascular-like network structures, albeit that sectioning inevitably led to the loss of 3D structure. Conclusions: Except for OCT, all methods had complementary merit and generated qualitative and quantitative data that allowed us to understand vascular network formation in organ-derived ECM hydrogels.

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Insulin modified Decellularized Adipose Tissue/Tremella Polysaccharide hydrogel loaded with ADSCs for skin wound healing

Dong

Kong

Jiang

et al. 2023

Biochemical and Biophysical Research Communications

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An overview of post transplantation events of decellularized scaffolds

Cited by 9 publications

References 104 publications

Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications

Regenerative Potential of A Bovine ECM-Derived Hydrogel for Biomedical Applications

From Macro to Micro: Comparison of Imaging Techniques to Detect Vascular Network Formation in Left Ventricle Decellularized Extracellular Matrix Hydrogels

Insulin modified Decellularized Adipose Tissue/Tremella Polysaccharide hydrogel loaded with ADSCs for skin wound healing

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