BACKGROUNDStrawberry is a rich source of antioxidants, including ascorbic acid (ASA) and polyphenols, which have numerous health benefits. Antioxidant content and activity are often determined manually using laboratory equipment, which is destructive and time‐consuming. This study constructs a prediction model for antioxidant compounds utilizing machine learning (ML) and multiple linear regression based on environmental, plant growth and agronomic fruit quality‐related parameters as well as antioxidant levels. These were studied in three farms at two‐week intervals during two years of cultivation.RESULTSDuring the ML model screening, artificial neural network (ANN)‐boosted models displayed a moderate coefficient of determination (R2) at 0.68–0.78 and relative root mean square error (RRMSE) at 3.8–4.8% in polyphenols and total ASA levels, as well as a high R2 of 0.96 and low RRMSE at <3.0% in antioxidant activity. Additionally, we developed variable selection models regarding the antioxidant activity, and variables two and five (environmental parameters and leaf length, respectively) with high accuracy were selected. The linear regression analysis between the actual and predicted data of antioxidants in the ANN‐boosted models revealed high fitness with all parameters in almost all training, validation and test sets. Furthermore, environmental parameters are essential in developing such reliable models.CONCLUSIONWe conclude that ANN‐boosted, stepwise and double‐Lasso regression models can predict antioxidant compounds with enhanced accuracy, and the relevant parameters can be easily acquired on‐site without the need for any specific equipment. © 2024 Society of Chemical Industry.