An overview of sequential injection chromatography

Chocholouš, Petr; Solich, Petr; Šatı́nský, Dalibor

doi:10.1016/j.aca.2007.02.018

Cited by 97 publications

(50 citation statements)

References 35 publications

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“…Their extreme versatility distinguishes them from other analytical techniques because they provide fast, accurate, and precise results. Sequential injection chromatography (SIC) is a well-established technique that integrates a short separation column into a sequential injection analysis (SIA) flow system (Chocholouš, Solich, and Šatínský 2007). SIC has already been consolidated as a good alternative to high performance liquid chromatography (HPLC) for the analysis of mixtures at low-pressures (Chocholouš, Solich, and Šatínský 2007;González-San Miguel et al 2009;Idris 2010).…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

et al. 2015

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The synthesis of amide (butyl, cyclohexyl, and phenyl) modified silica and the use as stationary phases in sequential injection chromatography are described. The system was tested on the isocratic separation of seven sulfonamides (sulfachloropyridazine, sulfadimethoxine, sulfamethazine, sulfamethoxazole, sulfamethoxypyridazine, sulfaquinoxaline, and sulfathiazole) using each stationary phase with mobile phases composed of acetonitrile/water at a flow rate of 0.45 mL min −1. A mixed mode retention mechanism of sulfonamides in the stationary phases was obtained, including dipole-dipole, π-π, and hydrogen bonding interactions. The most appropriate phase for the separation of sulfonamides was phenylamide. The chromatographic behavior was confirmed using density functional theory of the interaction between sulfamethoxazole and the stationary phases. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

et al. 2015

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“…Electroanalytical methods are known to attend the demand for minimal sample treatment and low consumption of organic solvents, 12,13 but these techniques are liable to matrix effects such as passivation 15 and the low backpressures provided by C 18 monolithic stationary phases. This technique uses low cost instrumentation and has been applied especially in the determination of components of pharmaceutical products, 16 although determinations of herbicides and pesticides 17,18 and amino acids 19 have been described. The present paper shows that the SIC capabilities for separation of simple mixtures can be explored for monitoring the maximum concentration levels of picloram in natural waters.…”

Section: Introductionmentioning

confidence: 99%

Determination of picloram in waters by sequential injection chromatography with UV detection

Santos¹,

Infante²,

Masini³

2010

J. Braz. Chem. Soc.

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Este trabalho descreve um procedimento de cromatografia por injeção seqüencial para a determinação de picloram em águas explorando a baixa pressão de uma coluna monolítica C 18 de 2,5 cm de comprimento. A separação do analito da matriz foi obtida em menos de 60 s usando como fase móvel uma mistura de acetonitrila e H 3 PO 4 5,0 mmol L -1 na proporção 20:80 (v v -1 ) e vazão de 30 mL s -1. Detecção foi feita a 223 nm com uma cela de 40 mm de caminho óptico. O limite de detecção do método é adequado para monitorar o nível de concentração máximo permitido para picloram em água potável (500 mg L -1 ). A frequência de amostragem é de 60 análises por hora, consumindo 300 mL de acetonitrila por análise. A metodologia foi aplicada a águas de rio fortificadas, não sendo observadas diferenças estatisticamente significativas em comparação com a metodologia convencional de HPLC-UV. This paper describes a sequential injection chromatography procedure for determination of picloram in waters exploring the low backpressure of a 2.5 cm long monolithic C 18 column. Separation of the analyte from the matrix was achieved in less than 60 s using a mobile phase composed by 20:80 (v v ). The sampling frequency is 60 analyses per hour, consuming only 300 mL of acetonitrile per analysis. The proposed methodology was applied to spiked river water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method.Keywords: sequential injection chromatography, monolithic column, picloram, waters IntroductionThe presence of pesticides in surface and ground waters is a consequence of the extensive use of these chemicals in agriculture and their runoff down through the soil profile. Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxilic acid) is a herbicide widely used to control weeds in crops of sugar cane (pre-emergency), rice, pasture and wheat (pos-emergency).2 This herbicide can stay active in soil for long time, depending on the type of soil, soil moisture and temperature. It may exist at toxic levels to plants for more than one year after application at normal rates. 2,3 It is chemically adsorbed onto clay particles and natural organic matter occurring in soils. If the soil is poor in clay or organic matter contents, the herbicide may be easily leached to surface and ground waters. 3-6Determination of picloram is usually made by gas-liquid chromatography with electron capture detector or mass spectrometry detectors, 7 although several high performance liquid chromatography methods have already been proposed using either UV absorption or mass spectrometry detection modes. [8][9][10][11] These methods are very sensitive, but require the use of large sample volumes, besides to extensive extraction steps, derivatization reactions and expensive instrumentation, so that new sensitive methods that reduce the time of analysis and the use of organic solvents are needed. Electroanalytical methods are known to attend the demand for minimal sample treatment and low consumption of organic solvent...

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“…Fast separations and short analysis cycles are based on the short length of the monolithic columns, while chromatographic efficiency depends on the selection of the mobile phase and on the properties of the analytes. The interested reader can find useful information on SIC in two excellent review articles that have been published recently [10,11].…”

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confidence: 99%

Automated Quality Control of Pharmaceuticals using Sequential Injection Chromatography (SIC)

Tzanavaras¹

2012

Pharm Anal Acta

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An overview of sequential injection chromatography

Cited by 97 publications

References 35 publications

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

Synthesis and Characterization of Amide Stationary Phases for the Determination of Sulfonamides by Sequential Injection Chromatography

Determination of picloram in waters by sequential injection chromatography with UV detection

Automated Quality Control of Pharmaceuticals using Sequential Injection Chromatography (SIC)

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