An Overview of Subnanosecond Pulsed Electric Field Biological Effects: Toward Contactless Technologies for Cancer Treatment

Ibrahimi, Njomza; Vallet, Leslie; André, Franck; Rivaletto, Marc; Novac, B.M.; Mir, Lluis M.; Pécastaing, Laurent

doi:10.1089/bioe.2022.0031

Cited by 1 publication

(1 citation statement)

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“…In addition, the effects of nsPEF were rarely studied on realistic three-dimensional in vitro cancer models, such as multicellular spheroids. Shortening pulse duration is also very attractive to possibly reach deep-seated tumors using contactless technologies [29]. Indeed, when the pulse width is reduced, the dielectric properties of cells become important, paving the way toward propagative delivery systems that have the considerable advantage of eliminating the need for surgery to insert the electrodes [29].…”

Section: Discussionmentioning

confidence: 99%

Effects of Nanosecond Pulsed Electric Field (nsPEF) on a Multicellular Spheroid Tumor Model: Influence of Pulse Duration, Pulse Repetition Rate, Absorbed Energy, and Temperature

Orlacchio,

Kolosnjaj-Tabi,

Mattei

et al. 2023

IJMS

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Cellular response upon nsPEF exposure depends on different parameters, such as pulse number and duration, the intensity of the electric field, pulse repetition rate (PRR), pulsing buffer composition, absorbed energy, and local temperature increase. Therefore, a deep insight into the impact of such parameters on cellular response is paramount to adaptively optimize nsPEF treatment. Herein, we examined the effects of nsPEF ≤ 10 ns on long-term cellular viability and growth as a function of pulse duration (2–10 ns), PRR (20 and 200 Hz), cumulative time duration (1–5 µs), and absorbed electrical energy density (up to 81 mJ/mm3 in sucrose-containing low-conductivity buffer and up to 700 mJ/mm3 in high-conductivity HBSS buffer). Our results show that the effectiveness of nsPEFs in ablating 3D-grown cancer cells depends on the medium to which the cells are exposed and the PRR. When a medium with low-conductivity is used, the pulses do not result in cell ablation. Conversely, when the same pulse parameters are applied in a high-conductivity HBSS buffer and high PRRs are applied, the local temperature rises and yields either cell sensitization to nsPEFs or thermal damage.

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Section: Discussionmentioning

confidence: 99%