An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Yi, Baolian; Xu, Qi; Liu, Wei

doi:10.1016/j.bioactmat.2021.12.005

Cited by 136 publications

(119 citation statements)

References 200 publications

Supporting

Mentioning

116

Contrasting

Order By: Relevance

“…Stiffness is an important structural clue for hydrogels used as tissue engineered substrate, correlated with scaffold dimensionality and cell types [157], affecting the cell adhesion, migration, differentiation, and ECM morphology. ECM is a dynamic network with molecular composition and matrix pliability modulated by the resident cells.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…ECM is a dynamic network with molecular composition and matrix pliability modulated by the resident cells. Inside the human body, the ECM stiffness (measured through Young's modulus, a measure of the resistance to deformation) presents different values (see Figure 2 from [157]). Thus, it varies from brain (1-3 kPa) and muscle (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42) to tendon (136-820 MPa) and bone .…”

Section: Mechanical Propertiesmentioning

confidence: 99%

“…Thus, it varies from brain (1-3 kPa) and muscle (23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42) to tendon (136-820 MPa) and bone . The bone is the hardest human tissue (15-40 GPa) [157,158], whereas for the demineralized bone matrix the stiffness decreases (~0.67 MPa [157,159]); the aggregation of endothelial cells in blood vessel is superior in stiff matrices (37.7 kPa) compared to soft matrices (13 kPa) [160]. The strategies adopted for regulation of the substrate stiffness were recently discussed [161]: controlled crosslinking density, molecular weight, and concentration of different constituents in multicomponent hydrogels; tunable intermolecular interactions, incorporation of NPs, design of the appropriate architecture using an adequate approach.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

See 2 more Smart Citations

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Bercea¹

2022

Polymers

View full text Add to dashboard Cite

Hydrogels, as interconnected networks (polymer mesh; physically, chemically, or dynamic crosslinked networks) incorporating a high amount of water, present structural characteristics similar to soft natural tissue. They enable the diffusion of different molecules (ions, drugs, and grow factors) and have the ability to take over the action of external factors. Their nature provides a wide variety of raw materials and inspiration for functional soft matter obtained by complex mechanisms and hierarchical self-assembly. Over the last decade, many studies focused on developing innovative and high-performance materials, with new or improved functions, by mimicking biological structures at different length scales. Hydrogels with natural or synthetic origin can be engineered as bulk materials, micro- or nanoparticles, patches, membranes, supramolecular pathways, bio-inks, etc. The specific features of hydrogels make them suitable for a wide variety of applications, including tissue engineering scaffolds (repair/regeneration), wound healing, drug delivery carriers, bio-inks, soft robotics, sensors, actuators, catalysis, food safety, and hygiene products. This review is focused on recent advances in the field of bioinspired hydrogels that can serve as platforms for life-science applications. A brief outlook on the actual trends and future directions is also presented.

show abstract

Section: Mechanical Propertiesmentioning

confidence: 99%

Section: Mechanical Propertiesmentioning

confidence: 99%

Section: Mechanical Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Bercea¹

2022

Polymers

View full text Add to dashboard Cite

show abstract

“…Biological cues can be integrated into polymer scaffold to create a mimic of the native extracellular matrix (ECM), which is the framework guiding the activity of immune cells [15, 16] . Formation of a pro-regenerative immune microenvironment might support the development of proper functional skin regeneration instead of fibrotic scar [17] .…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic graft transplantation is one of the efficient treatments for severe large area wound of skin especially when patient’s donor site is not qualified [3] . With the further understanding of tissue engineering, it is suggested that synthetic materials imitating the native structure of native ECM structure can integrate and play potentially pro-regeneration role to wound healing [15, 45] . Our previous report has illustrated the pro- regenerative immuno-regulatory effect of aligned electrospinning scaffold with approximate 300nm diameter fibers in small wound (diameter=0.6cm) healing [18, 19] .…”

Section: Introductionmentioning

confidence: 99%

Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration

YANG¹,

Chu²,

Hu³

et al. 2022

Preprint

View full text Add to dashboard Cite

Fibrotic scar is one of the primary impediments to severe skin wound healing, characterized by excessive deposition of extracellular matrix (ECM) devoid of skin appendages including hair follicle (HF). Intriguingly, functional hair follicles can regenerate specifically in the large wound center of adult mice, termed wound-induced hair neogenesis (WIHN). Single-cell RNA sequencing (scRNA-Seq) comparisons of small and large wound showed increased activation of adaptive immune systems which might associated with predominance of papillary fibroblast (PF) in large wound. Integration the gene expression profiles of spatial transcriptomic (ST) and scRNA-Seq highlighted a pro-regenerative immune niche, composing of T cells, pro-inflammatory macrophage (PIM) and monocyte surrounding the migrating hair follicle stem cell (HFSC) and PF in large wound center. Local pro-regenerative immune response predicted by Cellchat provided potential therapeutic target for the further HF regeneration. Aligned electrospinning scaffolds were previously shown to accelerate small wound healing with the immunomodulatory effect of T cells and macrophages. Here we showed that wound recapitulated normal skin architecture with the implantation of scaffold despite wound size. Enlarged distribution of the pro-regenerative immune niche co-localization with PF may account for the well-proportioned de novo HF in scaffold-implanted group. Collectively, our study illustrated that manipulating the adaptive immune system may stimulate de novo HF regeneration via local cell-cell interactions.

show abstract

Mechanisms for Biomaterials Reconstruct Microenvironment in Bone Regeneration

Wei¹,

Li²

2022

Biomaterials Effect on the Bone Microenvironment

View full text Add to dashboard Cite

An overview of substrate stiffness guided cellular response and its applications in tissue regeneration

Cited by 136 publications

References 200 publications

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Bioinspired Hydrogels as Platforms for Life-Science Applications: Challenges and Opportunities

Tracing immune cells around biomaterials with spatial anchors during large-scale wound regeneration

Mechanisms for Biomaterials Reconstruct Microenvironment in Bone Regeneration

Contact Info

Product

Resources

About