An Overview of the Automated and On-Line Systems to Assess the Oxidative Potential of Particulate Matter

Carlino, Alessandro; Romano, Maria Pia; Lionetto, Maria Giulia; Contini, Daniele; Guascito, Maria Rachele

doi:10.3390/atmos14020256

Cited by 9 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As regards the PM 10 components able to induce the activation of VRAC channels, it is known that these channels are activated by ROS in various cell types ( Jiao et al, 2006 ; Matsuda et al, 2010 ; Shen et al, 2014 ; Friard et al, 2021 ). PM is known to express an intrinsic oxidant-generating capacity that is related with the physical-chemical properties of the particles, such as their surface characteristics and their chemical composition, related to the pollutants adsorbed (such as metals, polycyclic aromatic hydrocarbons, quinones) expressed by the PM oxidative potential ( Chirizzi et al, 2017 ; Carlino et al, 2023 ). Besides its intrinsic oxidant-generating capacity, PM also exhibits a cell-mediated oxidant-generating capacity once in the cells, including the activation of intracellular signaling pathways leading to the formation of ROS, the interference with the endogenous production of ROS at the mitochondrial level, the release of radical metabolites arising from the biotransformation of the chemical contaminants of PM ( Ghio et al, 2012 ), and the inhibition of intracellular antioxidant defenses ( Chirino et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Apoptotic volume decrease (AVD) in A549 cells exposed to water-soluble fraction of particulate matter (PM10)

et al. 2023

View full text Add to dashboard Cite

Exposure to atmospheric particulate matter (PM) is recognized as a human health risk factor of great concern. The present work aimed to study the cellular mechanisms underlying cytotoxic effects of airborne particulate matter <10 µm in size (PM10), sampled in an urban background site from January to May 2020, on A549 cells. In particular, the study addressed if PM10 exposure can be a main factor in the induction of the Apoptotic Volume Decrease (AVD), which is one of the first events of apoptosis, and if the generation of intracellular oxidative stress can be involved in the PM10 induction of apoptosis in A549 cells. The cytotoxicity of PM10 samples was measured by MTT test on cells exposed for 24 h to the PM10 aqueous extracts, cell volume changes were monitored by morphometric analysis of the cells, apoptosis appearance was detected by annexin V and the induction of intracellular oxidative stress was evaluated by the ROS sensitive CM-H2DCFDA fluorescent probe. The results showed cytotoxic effects ascribable to apoptotic death in A549 cells exposed for 24 h to aqueous extracts of airborne winter PM10 samples characterized by high PM10 value and organic carbon content. The detected reduced cell viability in winter samples ranged from 55% to 100%. Normotonic cell volume reduction (ranging from about 60% to 30% cell volume decrease) after PM10 exposure was already detectable after the first 30 min clearly indicating the ability of PM10, mainly arising from biomass burning, to induce Apoptotic Volume Decrease (AVD) in A549 cells. AVD was prevented by the pre-treatment with 0.5 mM SITS indicating the activation of Cl− efflux presumably through the activation of VRAC channels. The exposure of A549 cells to PM10 aqueous extracts was able to induce intracellular oxidative stress detected by using the ROS-sensitive probe CM-H2DCFDA. The PM10-induced oxidative stress was statistically significantly correlated with cell viability inhibition and with apoptotic cell shrinkage. It was already evident after 15 min exposure representing one of the first cellular effects caused by PM exposure. This result suggests the role of oxidative stress in the PM10 induction of AVD as one of the first steps in cytotoxicity.

show abstract

Section: Discussionmentioning

confidence: 99%

Apoptotic volume decrease (AVD) in A549 cells exposed to water-soluble fraction of particulate matter (PM10)

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Cell-based assays investigate the production of ROS in animal (mainly rat, murine, and porcine) macrophage cell lines and transformed human bronchial epithelial cell lines that mimic the oxidative stress response of primary epithelial cells (Peixoto et al 2017 ; Wang et al 2018 ; Øvrevik 2019 ; He and Zhang 2023 ). Additionally, acellular assays that directly measure ROS (such as electron spin resonance (ESR), which measures the generation of hydroxyl radical (OH•) in the presence of H 2 O 2 using spectrometry) and acellular assays that indirectly measure ROS (such as the oxidation of dithiothreitol (DTT), ascorbic acid (AA), urate (UA), total glutathione and oxidized glutathione (GSSG)) have been developed and applied for the assessment of OP-PM (Bates et al 2019 ; Jiang et al 2019 ; Pietrogrande et al 2019 ; Øvrevik 2019 ; Gao et al 2020 ; Rao et al 2020 ; Khoshnamvand et al 2020 ; Liu and Chan 2022 ; Shahpoury et al 2022 ; Carlino et al 2023 ; He and Zhang 2023 ). However, there is still no clear consensus on the advantages, limitations, and applicability of these assays (Ayres et al 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Oxidative potential of the inhalation bioaccessible fraction of PM10 and bioaccessible concentrations of polycyclic aromatic hydrocarbons and metal(oid)s in PM10

Novo–Quiza,

Sánchez–Piñero,

Moreda–Piñeiro

et al. 2024

Environ Sci Pollut Res

View full text Add to dashboard Cite

Atmospheric particulate matter (PM) has been related to numerous adverse health effects in humans. Nowadays, it is believed that one of the possible mechanisms of toxicity could be the oxidative stress, which involves the development of reactive oxygen species (ROS). Different assays have been proposed to characterize oxidative stress, such as dithiothreitol (DTT) and ascorbic acid (AA) acellular assays (OPDTT and OPAA), as a metric more relevant than PM mass measurement for PM toxicity. This study evaluates the OP of the bioaccessible fraction of 65 PM10 samples collected at an Atlantic Coastal European urban site using DTT and AA assays. A physiologically based extraction (PBET) using Gamble’s solution (GS) as a simulated lung fluid (SLF) was used for the assessment of the bioaccessible fraction of PM10. The use of the bioaccessible fraction, instead of the fraction assessed using conventional phosphate buffer and ultrasounds assisted extraction (UAE), was compared for OP assessment. Correlations between OPDTT and OPAA, as well as total and bioaccessible concentrations of polycyclic aromatic hydrocarbons (PAHs) and metal(oid)s, were investigated to explore the association between those compounds and OP. A correlation was found between both OP (OPDTT and OPAA) and total and bioaccessible concentrations of PAHs and several metal(oid)s such as As, Bi, Cd, Cu, Ni, and V. Additionally, OPDTT was found to be related to the level of K+.

show abstract

“…Some studies suggest that ROS are a promising metric linking aerosol compositions to toxicity and adverse health effects (Zhang et al, 2021), and some publications are about measuring the oxidative potential in particulate matter (Frezzini et al, 2022). However, some limitations remain (Carlino et al, 2023). The oxidative potential was assessed as the ability of particles to generate ROS (Borlaza et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Study of the Oxidising Capacity of Different Materials in Relation With Silica Content

Garcia-Gonzalez,

Lopez-Pola,

Antuña-Jiméne

et al. 2023

International Symposium on Occupational Safety and Hygiene: Proceedings Book of the SHO2023

View full text Add to dashboard Cite

Background: Silica dust is a significant concern in the industry; it is clear that freshly fractured silica is more dangerous than aged fractured silica. However, it is not well studied if the oxidation-reduction potential (ORP) can be the key to the dangerousness of silica. Objective: Evaluate the oxidising capacity of four materials with different content in silica (SiO 2 ) (marble, granite, Silestone, quartz). Method: The materials were ground and tested using a portable handheld kit for ORP measurements at different ages. Results: The ORP values of the materials are increasingly oxidising until reaching 2-3 hours, where they get a stable ORP value. As the % of silicon increases, the oxidising power of the material also increases, except for the Silestone. Conclusion: There is a relation between the % of silica and the ORP, but some mixtures can reduce the ORP from materials with a high % of silica. Application: A well know ORP from the materials could establish different occupational limits for fresh or aged silica, but more research is required.

show abstract

An Overview of the Automated and On-Line Systems to Assess the Oxidative Potential of Particulate Matter

Cited by 9 publications

References 53 publications

Apoptotic volume decrease (AVD) in A549 cells exposed to water-soluble fraction of particulate matter (PM10)

Apoptotic volume decrease (AVD) in A549 cells exposed to water-soluble fraction of particulate matter (PM10)

Oxidative potential of the inhalation bioaccessible fraction of PM10 and bioaccessible concentrations of polycyclic aromatic hydrocarbons and metal(oid)s in PM10

Study of the Oxidising Capacity of Different Materials in Relation With Silica Content

Contact Info

Product

Resources

About