An overview of the phenotypic and genotypic characteristics of multidrug-resistant Mycobacterium tuberculosis isolates from four Asian countries

Ang, Concepcion; Ong, Chia Sui; Rukmana, Andriansjah; Thi, K. L. Pham; Yap, Sook Fan; Ngeow, Yun Fong; Ho, Meng Wei; Sudiro, Tjahjani Mirawati; Bela, Budiman; Jordaan, A M; Streicher, Elizabeth M.; Victor, Thomas C.

doi:10.1099/jmm.0.47850-0

Cited by 3 publications

(9 citation statements)

References 8 publications

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“…The Manila family, characterized by Douglas et al (7), is the predominant genotype seen in the Philippines and is common throughout the Pacific, Asia, and the western United States (1,14). It is defined as an ancestral strain, containing the TbD1 region, and all isolates share the same spoligotype (3,4).…”

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confidence: 99%

Rapid Deletion-Based Subtyping System for the Manila Family of Mycobacterium tuberculosis

Frink

Qian

et al. 2011

J Clin Microbiol

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The Manila family of Mycobacterium tuberculosis is a group of clonal isolates seen throughout the Pacific Basin. Commonly used rapid molecular typing methods often leave large groups of Manila family isolates clustered together. Here we describe a simple deletion-based PCR method that improves the discrimination for Manila family isolates, with or without the use of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) classification, and that is both rapid and affordable. Twenty-eight Manila family isolates, classified by spoligotyping, were collected from around the Pacific Basin from 1995 to 2003 and were tested for known genomic deletions. Nine of 15 regions of difference tested were identified as potentially discriminatory, with 18 distinct patterns; of these 9, 5 were selected for optimal discrimination using 61 Manila family isolates collected from California in 2009. For this geographically limited sample, the single large cluster was reduced to 14 distinct patterns. When the isolates were tested by spoligotyping and MIRU-VNTR, the addition of deletion analysis increased the number of distinct patterns from 43 to 56. In summary, the two study groups, which together form a single group of 89 isolates by spoligotyping, were segregated into 17 subgroups by our deletion-based subtyping system.

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confidence: 99%

Rapid Deletion-Based Subtyping System for the Manila Family of Mycobacterium tuberculosis

Frink

Qian

et al. 2011

J Clin Microbiol

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“…General Procedure for the Preparation of 3,4-Dichloro-5-aryloxy-2(5H)-furanones [17][18][19]. To a solution of mucochloric acid (1 equiv, 11.8 mmol) in tetrahydrofuran (20 mL), stirred at room temperature, was added the desired alcohol (0.75 equiv) and followed by drop-wise addition of concentrated sulfuric acid (2.00 mL).…”

Section: Synthetic Chemistrymentioning

confidence: 99%

“…The strains included rifampicin mono-resistant (RIF R ) and isoniazid mono-resistant (INH R ) strains, resistant to 2 and 0.1 mg/mL (RIF R and INH R ), respectively. The nature of the mutation conferring RIF R was rpoB (1S/531TTG) and the mutation conferring INH R was either inhA promoter (R1845) or katG (R1129 1S/315ACC) as determined by mutational analysis (18). Selected Gram-negative bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC9027, Klebsiella pneumoniae ATCC13883) and Gram-positive bacteria (Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC 29212, Bacillus cereus ATCC 11778) were grown in Tryptone Soya Broth (Difco Laboratories, Detroit) and were used for selectivity testing.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

“…Clinical isolates of M. tuberculosis were identified as drug resistant by mutational analysis and genotyping (18). Multidrug-resistant (MDR) isolates were confirmed resistant at the critical concentrations of isoniazid (0.2 mg/mL), rifampicin (2.0 mg/mL) and ethambutol (2.5 mg/mL) (21).…”

Section: Antibacterial Activity and Minimum Inhibitory Concentration mentioning

confidence: 99%

“…All isolates were obtained from a genotyped strain collection in the Department of Biomedical Sciences, University of Stellenbosch (18). The isolates were characterized as resistant or susceptible based on growth at critical concentrations of first line antibiotics.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

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Design, synthesis, andIn vitroantituberculosis activity of 2(5H)-Furanone derivatives

Ngwane

Panayides²,

Chouteau

et al. 2016

IUBMB Life

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A series of 2(5H)-furanone-based compounds were synthesized from commercially available mucohalic acids. From the first-generation compounds, three showed inhibitory activity (10 mg/mL) of at least 35% against Mycobacterium smegmatis mc 2 155 growth (Bioscreen C system). In screening the active first-generation compounds for growth inhibition against Mycobacterium tuberculosis H37Rv, the most active compound was identified with a minimum inhibitory concentration (MIC 99 ) of 8.07 mg/mL (15.8 mM) using BACTEC 460 system. No cross-resistance was observed with some current first-line anti-TB drugs, since it similarly inhibited the growth of multidrug resistant (MDR) clinical isolates. The compound showed a good selectivity for mycobacteria since it did not inhibit the growth of selected Gram-positive and Gram-negative bacteria. It also showed synergistic activity with rifampicin (RIF) and additive activity with isoniazid (INH) and ethambutol (EMB). Additional time-kill studies showed that the compound is bacteriostatic to mycobacteria, but cytotoxic to the Chinese Additional Supporting Information may be found in the online version of this article. 612 IUBMB LifeHamster Ovarian (CHO) cell line. From a second generation library, two compounds showed improved anti-TB activity against M. tuberculosis H37Rv and decreased CHO cell cytotoxicity. The compounds exhibited MIC values of 2.62 mg/mL (5.6 mM) and 3.07 mg/mL (5.6 mM) respectively. The improved cytotoxicity against CHO cell line of the two compounds ranged from IC 50 5 38.24 mg/mL to IC 50 5 45.58 mg/mL when compared to the most active first-generation compound (IC 50 5 1.82 mg/mL). The two second generation leads with selectivity indices (SI) of 14.64 and 14.85 respectively, warrant further development as anti-TB drug candidates. V C 2016 IUBMB Life, 68(8):612-620, 2016

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Determinants of Multidrug-Resistant Tuberculosis Clusters, California, USA, 2004–2007

Metcalfe¹,

Kim²,

Lin³

et al. 2010

Emerg. Infect. Dis.

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An overview of the phenotypic and genotypic characteristics of multidrug-resistant Mycobacterium tuberculosis isolates from four Asian countries

Cited by 3 publications

References 8 publications

Rapid Deletion-Based Subtyping System for the Manila Family of Mycobacterium tuberculosis

Rapid Deletion-Based Subtyping System for the Manila Family of Mycobacterium tuberculosis

Design, synthesis, andIn vitroantituberculosis activity of 2(5H)-Furanone derivatives

Determinants of Multidrug-Resistant Tuberculosis Clusters, California, USA, 2004–2007

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