An overview of the transcriptional responses of two tolerant and susceptible sugarcane cultivars to borer (Diatraea saccharalis) infestation

Mello, Uilian Stefanello de; Vidigal, Pedro Marcus Pereira; Vital, Camilo Elber; Tomaz, Adriano Cirino; Figueiredo, Milene de; Peternelli, Luiz Alexandre; Barbosa, Márcio Henrique Pereira

doi:10.1007/s10142-020-00755-8

Cited by 12 publications

(9 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, the resistant line had comparatively more DEGs than did the susceptible line under P. xanthii invasion. This finding was similar to those found in previous transcriptomic studies in sorghum, sugarcane and wheat, in which resistant lines induced expression of more DEGs than did susceptible lines under biotic stress ( Kiani and Szczepaniec, 2018 ; De Mello et al, 2020 ; Kiani et al, 2021 ).…”

Section: Discussionsupporting

confidence: 91%

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Meng

Song

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Powdery mildew (PM) caused by Podosphaera xanthii poses a continuous threat to the performance and yield of the cucumber (Cucumis sativus L.). Control in the initial stages of infection is particularly important. Here, we studied the differential physiological and transcriptomic changes between PM-resistant strain B21-a-2-1-2 and PM-susceptible strain B21-a-2-2-2 at the early stage of P. xanthii attack. When challenged with P. xanthii, the tolerant line can postpone the formation of the pathogen primary germ. Comparative transcriptomic analysis suggested that DEGs related to the cell wall and to pathogen and hormone responses were similar enriched in both cucumber lines under P. xanthii infection. Notably, the number of DEGs triggered by P. xanthii in B21-a-2-1-2 was quintuple that in B21-a-2-2-2, revealing that the success of defense of resistant cucumber is due to rapidly mobilizing multiple responses. The unique responses detected were genes related to SA signaling, MAPK signaling, and Dof and WRKY transcription factors. Furthermore, 5 P. xanthii -inducible hub genes were identified, including GLPK, ILK1, EIN2, BCDHβ1, and RGGA, which are considered to be key candidate genes for disease control. This study combined multiple analytical approaches to capture potential molecular players and will provide key resources for developing cucumber cultivars resistant to pathogen stress.

show abstract

Section: Discussionsupporting

confidence: 91%

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Meng

Song

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…These results indicated that the resistant wheat responds to the mite feeding by eliciting a higher number of unique responses than the susceptible genotype. A higher number of DEGs in the resistant genotype in response to herbivory has been reported in several other species, such as in resistant sugarcane in response sugarcane borer, Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae) infestation [ 39 ], and in sorghum in response to sugarcane aphid sugarcane aphid, Melanaphis sacchari Zehtner (Hemiptera: Aphididae) [ 40 ], suggesting that resistant plants undergo significantly greater metabolic changes, which may help them respond more effectively to herbivory by activating defense-related genes.…”

Section: Resultsmentioning

confidence: 96%

“…Sequence reads were imported into the CLC Genomics Workbench version 20 (Qiagen, Valencia, CA, USA) and mapped to two versions of Triticum aestivum reference genomes, including Triticum aestivum v2.2 ( ; accessed on 6 January 2020 [ 39 ]) and IWGS RefSeq v.1.0 ( ; accessed on 6 January 2020 [ 40 ]). Based on the total read counts for each annotated gene, differential gene expression analyses were conducted using the Empirical Analysis of the DGE tool, which implements the “Exact Test” for two group comparisons [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Transcriptional Responses of Resistant and Susceptible Wheat Exposed to Wheat Curl Mite

Kiani

Bryan²,

Rush³

et al. 2021

IJMS

View full text Add to dashboard Cite

(1) Background: The wheat curl mite (Aceria tosichella Keifer) is a key pest of wheat (Triticum aestivum L.) worldwide. While a number of wheat cultivars resistant to the mites have been employed to minimize the impact on the yield and quality of grain, little is known regarding the mechanisms underlying host plant resistance. Therefore, the goal of this study was to explore changes in transcriptome of resistant and susceptible wheat in order to quantify the molecular changes that drive host plant resistance. (2) Methods: Two varieties, wheat curl mite-susceptible (Karl 92) and wheat curl mite-resistant (TAM112) wheat, both at 2-week postemergence, were used in this study. Half of the plants were exposed to wheat curl mite herbivory and half remained mite-free and served as controls. Transcriptome changes were quantified using RNA-seq and compared among treatments to identify genes and pathways affected by herbivores. (3) Results: We identified a number of genes and pathways involved in plant defenses against pathogens, herbivores, and abiotic stress that were differentially expressed in the resistant wheat exposed to wheat curl mite herbivory but were unaffected in the susceptible wheat. (4) Conclusions: Our outcomes indicated that resistant wheat counteracts wheat curl mite exposure through effective induction of genes and pathways that enhance its defense responses.

show abstract

“…Therefore, the green control of the stem borer has received a lot of attention from the community ( Gao et al, 2016 ; Cristofoletti et al, 2018 ). At present, researchers engaged in microbiome control have screened out parasitic microorganisms, such as Pseudomonas aeruginosa , that had significant killing power against the pest for control purposes ( Chen et al, 2012 ; Cristofoletti et al, 2018 ; de Mello et al, 2020 ). However, the activity of microbiome pesticides varies from region to region due to several factors such as geography.…”

Section: Introductionmentioning

confidence: 99%

Correlation of microbiomes in “plant-insect-soil” ecosystem

Liu

Zhao

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionTraditional chemical control methods pose a damaging effect on farmland ecology, and their long-term use has led to the development of pest resistance.MethodsHere, we analyzed the correlations and differences in the microbiome present in the plant and soil of sugarcane cultivars exhibiting different insect resistance to investigate the role played by microbiome in crop insect resistance. We evaluated the microbiome of stems, topsoil, rhizosphere soil, and striped borers obtained from infested stems, as well as soil chemical parameters.Results and DiscussionResults showed that microbiome diversity was higher in stems of insect-resistant plants, and contrast, lower in the soil of resistant plants, with fungi being more pronounced than bacteria. The microbiome in plant stems was almost entirely derived from the soil. The microbiome of insect-susceptible plants and surrounding soil tended to change towards that of insect-resistant plants after insect damage. Insects’ microbiome was mainly derived from plant stems and partly from the soil. Available potassium showed an extremely significant correlation with soil microbiome. This study validated the role played by the microbiome ecology of plant–soil-insect system in insect resistance and provided a pre-theoretical basis for crop resistance control.

show abstract

An overview of the transcriptional responses of two tolerant and susceptible sugarcane cultivars to borer (Diatraea saccharalis) infestation

Cited by 12 publications

References 73 publications

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Transcriptional Responses of Resistant and Susceptible Wheat Exposed to Wheat Curl Mite

Correlation of microbiomes in “plant-insect-soil” ecosystem

Contact Info

Product

Resources

About