An overview of three biocatalysts of pharmaceutical importance synthesized by microbial cultures

Dahiya, Divakar; Nigam, Poonam Singh Nee

doi:10.3934/microbiol.2021009

Cited by 8 publications

(6 citation statements)

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“…The development of solvent-stable xylanases has also been the focus of several published studies (Dahiya and Nigam, 2021). These studies shed light on the characteristics and potential applications of xylanases that exhibit stability in different solvent environments.…”

Section: Discussionmentioning

confidence: 99%

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Hassan Al-Shawi

2023

Catrina: The International Journal of Environmental Sciences

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The objective of this study was to evaluate the synthesis of xylanase enzyme by Bacillus safensis MABS6 using sorghum straw as a substrate. A comprehensive investigation was conducted to optimize xylanase yield by examining various parameters such as sorghum concentration, inoculum size, culture age, pH, temperature, and agitation speed. Additionally, the impact of nutritional additives, metallic ions, organic solvents, and alkaline H 2 O 2 treatment on xylanase production and activity was explored. Experimental trials were performed with specific parameters, including 4% w/v sorghum concentration, 2% inoculum size, 12 hours of culture age, pH 7, 35°C, and 250 rpm. Further improvements involved the addition of nutritional additives such as gelatin, xylose, and potassium nitrate. The effects of initial conditions, various metallic ions (Ca 2+ , Mn 2+ , and Fe 3+ ), and organic solvents (2-methyl propanol, methanol, and ethanol) on xylanase activity were evaluated. Additionally, saccharification investigations with a 4% alkaline H2O2 treatment assessed the enzymatic hydrolysis of sorghum straw. Optimized conditions resulted in a significant increase, with xylanase production reaching 3.49%. The partially purified xylanase exhibited over 60% relative activity within a pH range of 6 to 9 and demonstrated more than 55% activity between 45°C to 65°C after 1 hour. Certain metallic ions and organic solvents further enhanced xylanase activity. Notably, the xylanase derived from Bacillus safensis MABS6, utilizing sorghum straw, showcased desirable characteristics such as heat stability, alkali-solvent stability, and absence of cellulase activity. Its potential as a biocatalyst makes it valuable for various biotechnological applications, particularly in the efficient enzymatic hydrolysis of sorghum straw.

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Section: Discussionmentioning

confidence: 99%

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Hassan Al-Shawi

2023

Catrina: The International Journal of Environmental Sciences

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“…Since the supply of polysaccharides from vegetative sources is not realistic for obtaining materials of desired specifications with structural uniformity throughout the year, frequent studies were aimed at the biosynthesis of dextran in a controlled optimized process of bacterial culture cultivation for its sufficient availability. Microbial fermentative processes employing purposely modified efficient microbial strains have been reported to be cost-effective and can be manipulated for the biosynthesis of products of commercial importance using cheaper raw materials; similarly, dextran can be synthesized from sucrose [107][108][109][110]. Selected bacterial strains under customized fermentation conditions can be cultivated to synthesize a good yield of dextran in the microbial production process.…”

Section: Discussionmentioning

confidence: 99%

Dextran of Diverse Molecular-Configurations Used as a Blood-Plasma Substitute, Drug-Delivery Vehicle and Food Additive Biosynthesized by Leuconostoc, Lactobacillus and Weissella

Dahiya,

Nigam

2023

Applied Sciences

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Dextran, a microbial metabolite of diverse molecular configurations, can be biosynthesized employing selected strains of characterized species of bacteria. Dextran molecules are secreted as an extracellular polysaccharide in the culture medium of the bacterial fermentation system. This microbially produced polymer of glucose possesses multi-faceted characteristics such as its solubility in different solvents and formation of dextran solutions of needed viscosity. Several preparations can be formulated for the desired thermal and rheological properties. Due to such multifunctional characteristics, dextran with different structural specifications is a desired polysaccharide for clinical, pharmaceutical, and food industry commercial applications. Dextran and its derivative products with various molecular weights, in a range of high and low, have established their uses in drug delivery and in analytical devices using columns packed with polysaccharide gel. Therefore, being a neutral raw material, the resourcefulness of dextran preparations of different molecular weights and linkages in their polymer configuration is important. For this purpose, several studies have been performed to produce this commercially important polysaccharide under optimized bacterial cultivation processes. This article aims to overview recently published research reports on some significant applications of dextran in the pharmaceutical and food industries. Studies conducted under optimized conditions in fermentation processes for the biosynthesis of dextran of diverse molecular configurations, which are responsible for its multifunctional properties, have been summarized. Concise information has been presented in three separate tables for each group of specific bacterial species employed to obtain this extracellular microbial polysaccharide.

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“…There are other types of ester-forming reactions catalyzed by esterases, each reaction is performed under specific conditions [1,[14][15][16][17]:…”

Section: Catalytic Process Of Esterase Enzymementioning

confidence: 99%

“…Enzymes have been widely studied for their biochemistry, specific reactivity, and contribution to biochemistry and biological systems. Enzymes from microbial sources have been used for their important contribution as biocatalysts to synthesise added-value metabolites, as well as being used as an important tool in the area of diagnostics and therapeutics to improve human health [1]. The processes could be catalysed by biocatalysts as actively growing microbial cells, either in the form of freely suspended in the reaction medium or in immobilised form for their reuse.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

Dahiya

Nigam

2022

Sustainability

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Reactions catalysed by sustainably produced enzymes can contribute to the bioeconomy supporting several industries. Low-value compounds can be transformed into added-value products or high-resolution chemicals could be prepared in reactions catalysed by biocatalyst esterase enzymes. These enzymes can be synthesised by purposely isolated or genetically modified strains of microorganisms. Enzymes belonging to the hydrolase family catalyse the formation and hydrolysis of ester bonds to produce the desired esterified molecule. The synthesis of homo-chiral compounds can be accomplished either by chemical or biocatalytic processes, the latter being preferred with the use of microbial esterases. For varied applications, esterases with high stability and retained activity at lower and higher temperatures have been produced with strains isolated from extreme environments. For sustainable production of enzymes, higher productivity has been achieved by employing fast-growing Escherichia coli after incorporating plasmids of required characteristics from specific isolates. This is a review of the isolated and engineered strains used in the biosynthesis of esterase of the desired property, with the objective of a sustainable supply of enzymes, to produce products of industrial importance contributing to the economy.

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An overview of three biocatalysts of pharmaceutical importance synthesized by microbial cultures

Cited by 8 publications

References 50 publications

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Enhanced Xylanase Production from Bacillus safensis MABS6 using Sorghum Straw Substrate: Optimization, Characterization, and Biotechnological Applications

Dextran of Diverse Molecular-Configurations Used as a Blood-Plasma Substitute, Drug-Delivery Vehicle and Food Additive Biosynthesized by Leuconostoc, Lactobacillus and Weissella

Sustainable Biosynthesis of Esterase Enzymes of Desired Characteristics of Catalysis for Pharmaceutical and Food Industry Employing Specific Strains of Microorganisms

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