An Overview of Triticale Doubled Haploids

Eudes, François; Chugh, Archana

doi:10.1007/978-1-4020-8854-4_6

Cited by 18 publications

(7 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In triticale, the microspores density of 3 × 10 4 to 2 × 10 5 microspores/ml of the induction media is considered optimum for normal growth and development of microspores (Eudes and Chugh 2009 ) while densities of 8-10 × 10 4 microspores/ml for Capsicum annum (Kim et al 2008 ), 4 × 10 4 microspores/ml for B . napus (Huang et al 1990 ), and 5 × 10 4 microspores/ml for B .…”

Section: Media Compositionmentioning

confidence: 99%

Androgenesis: A Fascinating Doubled Haploid Production Process

Asif

2013

Progress and Opportunities of Doubled Haploid Production

View full text Add to dashboard Cite

Section: Media Compositionmentioning

confidence: 99%

Androgenesis: A Fascinating Doubled Haploid Production Process

Asif

2013

Progress and Opportunities of Doubled Haploid Production

View full text Add to dashboard Cite

“…Для получения гаплоидов тритикале успешно применяется метод культуры пыльников (Ignatova, 2011;Dya-tchouk et al, 2012;Krzewska et al, 2012;Wedzony et al, 2015) и изолированных микроспор (Oleszezuk et al, 2004;Eudes, Chugs, 2009;Lantos et al, 2014). Существенным ограничением для практического использования этого метода является высокая частота альбинизма среди регенерантов.…”

Section: получение гаплоидов тритикалеunclassified

Distant hybridization as a method of haploid production in cereals

Дьячук

Akinina

Хомякова

et al. 2019

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

Elimination of chromosomes is a phenomenon widespread in distant hybrids. It ranges from the loss of one or two chromosomes to elimination of whole chromosome complement of one of the parents. Such elimination leads to the production of haploid plants, which then are treated with colchicine to double the chromosome number and to develop DH‑lines. Homozygosity of doubled haploids serves as a basis for their wide use in plant genetics and breeding. The use of this approach reduces the time required for obtaining homozygous lines by 5 years on the average. It leads to savings in human resources, energy and acreage. The development of the “bulbosum” method for haploid barley production had a strong influence on the chromosome engineering in cereals and its implementation in plant breeding. However, the method developed on that basis could not be used effectively for producing haploids of wheat, triticale, etc. because of Hordeum bulbosum L. pollen sensitivity to genes inhibiting wheat crossability (Kr genes). The crosses with Imperata cylindrica (L.) Raeusch. is an efficient alternative to the widely used wheat × maize and triticale × maize crosses due to abundant pollen supply within a longer time period, significantly higher frequency of embryos formation and haploid plants regeneration.

show abstract

“…Triticale is an allopolyploid plant that was generated artificially [ 1 ] and is reasonably simple for in vitro plant regeneration [ 2 ], resulting in doubled haploid (DH) lines that could be utilized for the evaluation of new varieties [ 3 ]. Chromosome elimination [ 4 ] or microspore embryogenesis (androgenesis) [ 5 ] are two methods that could be used to create DH plants.…”

Section: Introductionmentioning

confidence: 99%

S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency

Orłowska

Żebrowski

Zimny

et al. 2022

Cells

View full text Add to dashboard Cite

The biological improvement of triticale, a cereal of increasing importance in agriculture, may be accelerated via the production of doubled haploid lines using in vitro culture. Among the relevant factors affecting the culture efficiency are Cu(II) or Ag(I) acting, e.g., as cofactors of enzymes. The copper ions are known to positively affect green plant regeneration efficiency. However, the biochemical basis, mainly its role in the generation of in vitro-induced genetic and epigenetic variation and green plant regeneration efficiency, is not well understood. Here, we employed structural equation modeling to evaluate the relationship between de novo DNA methylation affecting the asymmetric context of CHH sequences, the methylation-sensitive Amplified Fragment Length Polymorphism related sequence variation, and the concentration of Cu(II) and Ag(I) ions in induction media, as well as their effect on S-adenosyl-L-methionine perturbations, observed using FTIR spectroscopy, and the green plant regeneration efficiency. Our results allowed the construction of a theory-based model reflecting the biological phenomena associated with green plant regeneration efficiency. Furthermore, it is shown that Cu(II) ions in induction media affect plant regeneration, and by manipulating their concentration, the regeneration efficiency can be altered. Additionally, S-adenosyl-L-methionine is involved in the efficiency of green plant regeneration through methylation of the asymmetric CHH sequence related to de novo methylation. This shows that the Yang cycle may impact the production of green regenerants.

show abstract

An Overview of Triticale Doubled Haploids

Cited by 18 publications

References 26 publications

Androgenesis: A Fascinating Doubled Haploid Production Process

Androgenesis: A Fascinating Doubled Haploid Production Process

Distant hybridization as a method of haploid production in cereals

S-Adenosyl-L-Methionine and Cu(II) Impact Green Plant Regeneration Efficiency

Contact Info

Product

Resources

About