An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy

Abstract

In this study, 3D macroporous bioscaffolds were developed from poly(dimethylsiloxane) (PDMS) which is inert, biocompatible, non-biodegradable, retrievable and easily manufactured at low cost. PDMS bioscaffolds were synthesized using a solvent casting and particulate leaching (SCPL) technique and exhibited a macroporous interconnected architecture with 86 ± 3% porosity and 300 ± 100 μm pore size. As PDMS intrinsically has a hydrophobic surface, mainly due to the existence of methyl groups, its surface was modified by oxygen plasma treatment which, in turn, enabled us to apply a novel polydopamine coating onto the surface of the bioscaffold. The addition of a polydopamine coating to bioscaffolds was confirmed using composition analysis. Characterization of oxygen plasma treated-PDMS bioscaffolds coated with polydopamine (polydopamine coated-PDMS bioscaffolds) showed the presence of hydroxyl and secondary amines on their surface which resulted in a significant decrease in water contact angle when compared to uncoated-PDMS bioscaffolds (35 ± 3%, P < 0.05). Seeding adipose tissue-derived mesenchymal stem cells (AD-MSCs) into polydopamine coated-PDMS bioscaffolds resulted in cells demonstrating a 70 ± 6% increase in viability and 40 ± 5% increase in proliferation when compared to AD-MSCs seeded into uncoated-PDMS bioscaffolds (P < 0.05). In summary, this two-step method of oxygen plasma treatment followed by polydopamine coating improves the biocompatibility of PDMS bioscaffolds and only requires the use of simple reagents and mild reaction conditions. Hence, our novel polydopamine coated-PDMS bioscaffolds can represent an efficient and low-cost bioscaffold platform to support MSC therapies.

Accelerated construction of an in vitro model of human periodontal ligament tissue: vacuum plasma combined with fibronectin coating and a polydimethylsiloxane matrix

“…The chambers in this group had the highest wettability, highest O proportion, and lowest C proportion among all the chambers, and all the differences were statistically significant. The O proportion result indicated that the Si(-O 2 ) peak was much higher for the PN chambers compared with the other types; a hydrophilic silica-like layer had formed on the PDMS surface, which resulted in high wettability (Razavi & Thakor, 2018). The result that the lowest C proportion was in the PN chambers indicated that the methyl groups initially on the external surface of the chambers had moved to the internal surfaces (Fig.…”

Hydrogel Scaffolds to Deliver Cell Therapies for Wound Healing

“…Various studies have found that cells seeded on top of these materials will attach and proliferate normally ( Schaffer et al, 1994 ). Furthermore, these silicone membranes can foster both viability and proliferation of seeded ASCs ( Razavi and Thakor, 2018 ). The ability to treat and coat silicone and other biomaterials remains an attractive method to promote differentiation and growth, and these parameters may be tuned to optimize proper cell growth.…”

Peer Review #2 of "Accelerated construction of an in vitro model of human periodontal ligament tissue: vacuum plasma combined with fibronectin coating and a polydimethylsiloxane matrix (v0.1)"

“…The chambers in this group had the highest wettability, highest O proportion, and lowest C proportion among all the chambers, and all the differences were statistically significant. The O proportion result indicated that the Si(-O 2 ) peak was much higher for the PN chambers compared with the other types; a hydrophilic silica-like layer had formed on the PDMS surface, which resulted in high wettability (Razavi & Thakor 2018). The result that the lowest C proportion was in the PN chambers indicated that the methyl groups initially on the external surface of the chambers had moved to the internal surfaces (Figure 4).…”