An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice

He, Jun; Liu, Yuqiang; Yuan, Dingyang; Duan, Meijuan; Liu, Yanling; Shen, Zijie; Yang, Chen; Qiu, Zhiren; Liu, Daoming; Wen, Peizheng; Huang, Jie; Fan, Dejia; Xiao, Shizhuo; Xin, Yeyun; Chen, Xianian; Jiang, Ling; Wang, Haiyang; Yuan, Longping; Wan, Jianmin

doi:10.1073/pnas.1902771116

Cited by 192 publications

(170 citation statements)

References 36 publications

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“…However, in this study, the Ganoderma species had mostly 1R-MYBs, with no clearly conserved gene structure found. As the dominant 2R-MYBs play central roles in plant-specific processes (Lee & Seo 2019;He et al 2020), it is likely that the dominant 1R-MYBs might play core roles in relevant processes in Ganoderma species.…”

Section: Co-expression Analysis Of G Lucidum Myb Genesmentioning

confidence: 99%

“…Abundant evidence in plants has confirmed the diverse roles of MYB genes in signal transduction, developmental regulation, biotic and abiotic stress responses, disease resistance, and secondary metabolism regulation Lee & Seo 2019;Qing et al 2019;Wu et al 2019b;He et al 2020;Ohno et al 2020). Similarly, a few studies have shown that MYB genes participate in stress response and developmental regulation in fungi (Valsecchi et al 2017;Wang et al 2018;.…”

Section: Introductionmentioning

confidence: 96%

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Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Ganoderma Species

Wang

Huang

Zhang

et al. 2020

G3 Genes|Genomes|Genetics

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Numerous studies in plants have shown the vital roles of MYB transcription factors in signal transduction, developmental regulation, biotic/abiotic stress responses and secondary metabolism regulation. However, less is known about the functions of MYBs in Ganoderma. In this study, five medicinal macrofungi of genus Ganoderma were subjected to a genome-wide comparative analysis of MYB genes. A total of 75 MYB genes were identified and classified into four types: 1R-MYBs (52), 2R-MYBs (19), 3R-MYBs (2) and 4R-MYBs (2). Gene structure analysis revealed varying exon numbers (3-14) and intron lengths (7-1058 bp), and noncanonical GC-AG introns were detected in G. lucidum and G. sinense. In a phylogenetic analysis, 69 out of 75 MYB genes were clustered into 15 subgroups, and both single-copy orthologous genes and duplicated genes were identified. The promoters of the MYB genes harboured multiple cis-elements, and specific genes were co-expressed with the G. lucidum MYB genes, indicating the potential roles of these MYB genes in stress response, development and metabolism. This comprehensive and systematic study of MYB family members provides a reference and solid foundation for further functional analysis of MYB genes in Ganoderma species.

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Section: Co-expression Analysis Of G Lucidum Myb Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Ganoderma Species

Wang

Huang

Zhang

et al. 2020

G3 Genes|Genomes|Genetics

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“…Previous studies have shown that JA-and ET-mediated signaling pathways reduce rice resistance to BPH (Lu et al, 2014;Zhou et al, 2009), whereas SA-and H 2 O 2 -mediated pathways increase it (Hu et al, 2016;Wang, 2012). Recently, He et al (2020) found that phenylalanine ammonialyases, which are regulated by an R2R3 MYB transcription factor, OsMYB30, modulate the resistance of rice to BPH by regulating the biosynthesis and accumulation of salicylic acid and lignin. In this study, we also found that exogenously applying H 2 O 2 to WT plants enhances their resistance to BPH at the same levels reached by the ir-rlk2 lines ( Figure S12), confirming the important role of the H 2 O 2 pathway.…”

Section: F I G U R Ementioning

confidence: 99%

Suppression of a leucine‐rich repeat receptor‐like kinase enhances host plant resistance to a specialist herbivore

Kuai

et al. 2020

Plant Cell & Environment

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The mechanisms by which herbivores induce plant defenses are well studied. However, how specialized herbivores suppress plant resistance is still poorly understood. Here, we discovered a rice (Oryza sativa) leucine‐rich repeat receptor‐like kinase, OsLRR‐RLK2, which is induced upon attack by gravid females of a specialist piercing‐sucking herbivore, the brown planthopper (BPH, Nilaparvata lugens). Silencing OsLRR‐RLK2 decreases the constitutive activity of mitogen‐activated protein kinase (OsMPK6) and alters BPH‐induced transcript levels of several defense‐related WRKY transcription factors. Moreover, silencing OsLRR‐RLK2 reduces BPH‐induction of jasmonic acid and ethylene but promotes the biosynthesis of both elicited salicylic acid and H2O2; silencing also enhances the production of volatiles emitted from rice plants infested with gravid BPH females. These changes decrease BPH preference and performance in the glasshouse and the field. These findings suggest that OsLRR‐RLK2, by regulating the plant's defense‐related signaling profile, increases the susceptibility of rice to BPH, and that BPH infestation influences the expression of OsLRR‐RLK2, suppressing the resistance of rice to BPH.

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“…In our results laccase OsLAC5 was down-regulated in flag leaf and OsLAC4, a microRNA target gene on chromosome 1 was up-regulated in panicle. The PAL genes OsPAL6 and OsPAL8 mediate brown planthopper resistance by controlling lignin biosynthesis and its accumulation in rice 72 . Phenylalanine ammonia-lyase (OsPAL4) on chromosome 2 is associated with broad spectrum disease resistance in rice.…”

Section: Discussionmentioning

confidence: 99%

“…Both expression and sequence analysis indicates that OsPAL2 and OsPAL4 influence grain yield in test hybrid. Thus phenyl propanoid pathway and PAL genes in particular are at centre stage of not only broad spectrum disease 74 , pest resistance 72 and tolerance to abiotic stress 75 but also for increasing grain yield.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield

Guttikonda

Thummala

Agarwal

et al. 2020

Sci Rep

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In this study, we compared genome-wide transcriptome profile of two rice hybrids, one with (test hybrid IR79156A/IL50-13) and the other without (control hybrid IR79156A/KMR3) O. rufipogon introgressions to identify candidate genes related to grain yield in the test hybrid. IL50-13 (Chinsurah Nona2 IET21943) the male parent (restorer) used in the test hybrid, is an elite BC 4 f 8 introgression line of KMR3 with O. rufipogon introgressions. We identified 2798 differentially expressed genes (DEGs) in flag leaf and 3706 DEGs in panicle. Overall, 78 DEGs were within the major yield QTL qyld2.1 and 25 within minor QTL qyld8.2. The DEGs were significantly (p < 0.05) enriched in starch synthesis, phenyl propanoid pathway, ubiquitin degradation and phytohormone related pathways in test hybrid compared to control hybrid. Sequence analysis of 136 DEGs from KMR3 and IL50-13 revealed 19 DEGs with SNP/InDel variations. Of the 19 DEGs only 6 showed both SNP and InDel variations in exon regions. Of these, two DEGs within qyld2.1, Phenylalanine ammonia-lyase (PAL) (Os02t0626400-01, OsPAL2) showed 184 SNPs and 11 InDel variations and Similar to phenylalanine ammonia-lyase (Os02t0627100-01, OsPAL4) showed 205 SNPs and 13 InDel variations. Both PAL genes within qyld2.1 and derived from O. rufipogon are high priority candidate genes for increasing grain yield in rice. Rice (Oryza sativa) is an important food crop providing 20% of daily calories to more than 50 percent of global population. Nearly 90% of rice is produced and consumed in Asia. The continuous increase in human population, especially in Asia, poses a major challenge to food security. Therefore, enhancing grain yield is the primary thrust area of plant breeders. Hybrid rice can help increase productivity by 10-20% more than conventional varieties 1. Currently, the highest-yielding rice hybrids are developed from inter-subspecific crosses between indica and japonica 2-5. Wild species have been used to breed parental lines for yield improvement in derived rice hybrids 6-10. Flag leaf is the most essential functional organ to produce a large proportion of photo-assimilates that are stored in grains 11,12. It is estimated that it contributes around 32.3% of total carbohydrates during grain filling 13. The panicle morphology also directly affects the number and size of seeds and also determines grain yield in rice 14-16. Flag leaf and panicles have been used previously at different developmental stages to unravel gene expression in pollen development and genetic networks that control panicle branching and architecture 17,18. The spatial and temporal expression profiles of genes during 19 vegetative and reproductive stages of organ development were analysed to identify stage-preferential/stage-specific genes in IR64 variety and anther-specific genes in Pusa Basmati1 variety 19,20. A whole-genome oligonucleotide microarray of super hybrid LYP9 (Liangyoupeijiu) and its parents 9311 and PA64s in 7 different tissues showed that differentially expressed genes for energy metabolism i...

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An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice

Cited by 192 publications

References 36 publications

Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Ganoderma Species

Genome-Wide Characterization and Comparative Analysis of MYB Transcription Factors in Ganoderma Species

Suppression of a leucine‐rich repeat receptor‐like kinase enhances host plant resistance to a specialist herbivore

Genome-wide transcriptome profile of rice hybrids with and without Oryza rufipogon introgression reveals candidate genes for yield

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