An RME1-independent pathway for sporulation control in Saccharomyces cerevisiae acts through IME1 transcript accumulation.

Kao, Gautam; Shah, J C; Clancy, Mary J.

doi:10.1093/genetics/126.4.823

Cited by 12 publications

(6 citation statements)

References 37 publications

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“…The RNA was selectively precipitated with lithium chloride and ethanol following extraction with phenol and chloroform. RNAs were fractionated and blotted as described previously (21,49).…”

Section: Methodsmentioning

confidence: 99%

“…this study (Table 1) were constructed by standard techniques (14, 19, 37,40,44). Strains carrying the spr3::1acZ reporter were constructed by transplacement as described previously, using pGK11 (20,21). Haploid segregants carrying the RES1-1 mutation were detected by their ability to express the spr3::1acZ fusion at low levels, using a plate assay as described previously (21).…”

Section: Methodsmentioning

confidence: 99%

“…Strains lacking RMEJ or IMEJ activity were constructed as described previously, using pGK24 (21,30) or pAM506 (42), respectively, as the source of the null alleles. Strains that contained insertions at the IME4 locus were constructed by using pJS20, pJS21, pJS22, and pJS34 to generate the ime4::LEU2 (BglII), ime4::LEU2 (BamHI), IME4::LEU2 (PstI), and ime4::TRPI (EcoRI-SphI) alleles, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…An additional gene, RES1, was defined by a semidominant mutation (RES1-I) that allowed AMTaIAM Ta diploid cells to sporulate efficiently in the presence of excess copies of RMEI (21). This mutation also allows mat-insufficient cells to express IMEJ and to sporulate to low levels.…”

mentioning

confidence: 99%

“…Available genetic evidence has strongly suggested that additional MT-dependent functions also participate in the activation of the sporulation process. First, it has not been possible to mimick the MATa/MATa genotype fully by introducing the known bypass mutations into matinsufficient cells; nnel: :LEU2 mutations do not support wild-type levels of sporulation, even in combination with RESJ-J mutations (21,30). Conversely, overexpression of RMEI does not fully repress sporulation in ALTa/AL4Ta diploids (30).…”

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confidence: 99%

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IME4, a Gene That Mediates MAT and Nutritional Control of Meiosis in Saccharomyces cerevisiae

Shah

Clancy

1992

Molecular and Cellular Biology

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In the yeast Saccharomyces cerevisiae, sporulation occurs in response to nutritional and genetic signals. The process is initiated when nutrient availability limits mitotic growth, but only in MATa/MAT alpha diploid cells. Under these conditions, the cells express an activator of meiosis (IME1), which is required for the expression of early sporulation-specific genes. We describe a new gene, IME4, whose activity is essential for IME1 transcript accumulation and sporulation. The IME4 transcript was induced in starved MATa/MAT alpha diploids but not in other cell types. In addition, excess IME4 promoted sporulation in mat-insufficient cells. Thus, IME4 appears to activate IME1 in response to cell type and nutritional signals. We have also explored the interactions between IME4 and two genes that are known to regulate IME1 expression. Normally, cells that lack complete MAT information cannot sporulate; when such strains lack RME1 activity or contain the semidominant RES1-1 mutation, however, they can express IME1 and sporulate to low levels. Our results show that mat-insufficient strains containing rme1::LEU2 or RES1-1 bypass mutations still retain MAT control of IME4 expression. Even though IME4 levels remained low, the rme1::LEU2 and RES1-1 mutations allowed IME1 accumulation, implying that these mutations do not require IME4 to exert their effects. In accord with this interpretation, the RES1-1 mutation allowed IME1 accumulation in MATa/MAT alpha strains that contain ime4::LEU2 alleles. These strains still sporulated poorly, suggesting that IME4 plays a role in sporulation in addition to promoting IME1 transcript accumulation. IME4 is located between ADE5 and LYS5 on chromosome VII.

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“…The RNA was selectively precipitated with lithium chloride and ethanol following extraction with phenol and chloroform. RNAs were fractionated and blotted as described previously (21,49).…”

Section: Methodsmentioning

confidence: 99%