An Rna Aptamer Containing Two Binding Sites Against the HCV Minus-Ires Domain I

Konno, Keisuke; Iizuka, Mana; Fujita, Syusuke; Nishikawa, Satoshi; Hasegawa, Tsunemi; Fukuda, Kotaro

doi:10.1080/15257770.2011.562475

Cited by 8 publications

(9 citation statements)

References 33 publications

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“…Its consensus sequences 5'-UGGAUC-3' and 5'-GAGUAC-3', which were complementary to the SL-E1 and SL-D1 loops in the domain I were responsible for this effect. In this way they prevented attachment of viral RNA polymerase, NS5B, mentioned above [ 92 , 105 ]. Kikuchi et al obtained RNA aptamer containing loop structure with a consensus sequence 5'-UAUGGCU-3', complementary to the loop of the IRES domain II.…”

Section: Aptamers In the Treatment Of Viral Infectionsmentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

114

109

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Aptamers are in vitro selected DNA or RNA molecules that are capable of binding a wide range of nucleic and non-nucleic acid molecules with high affinity and specificity. They have been conducted through the process known as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). It serves to reach specificity and considerable affinity to target molecules, including those of viral origin, both proteins and nucleic acids. Properties of aptamers allow detecting virus infected cells or viruses themselves and make them competitive to monoclonal antibodies. Specific aptamers can be used to interfere in each stage of the viral replication cycle and also inhibit its penetration into cells. Many current studies have reported possible application of aptamers as a treatment or diagnostic tool in viral infections, e.g., HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus), HCV (Hepatitis C Virus), SARS (Severe Acute Respiratory Syndrome), H5N1 avian influenza and recently spread Ebola. This review presents current developments of using aptamers in the diagnostics and treatment of viral diseases.

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Section: Aptamers In the Treatment Of Viral Infectionsmentioning

confidence: 99%

Aptamers in Diagnostics and Treatment of Viral Infections

Wandtke

Woźniak

Kopiński

2015

Viruses

114

109

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“…Therefore, recruitment of ribosomal particles mediated by the IRES element was inhibited by the chimera HH363-24 that prevented both translation and replication in a hepatic cell line [95]. Moreover, to avoid HCV genome replication, Konno et al isolated RNA aptamers against the 3′ end of the negative strand of the virus genome [96,97]. Interestingly, a RNA aptamer, named AP30, was able to recognize this minus-IRES region and reduce positive-strand genomic RNA synthesis [96].…”

Section: Aptamers For Virus Diagnosis and Treatmentmentioning

confidence: 99%

“…Moreover, to avoid HCV genome replication, Konno et al isolated RNA aptamers against the 3′ end of the negative strand of the virus genome [96,97]. Interestingly, a RNA aptamer, named AP30, was able to recognize this minus-IRES region and reduce positive-strand genomic RNA synthesis [96]. To inhibit HCV replication, Marton et al selected RNA aptamers against CRE element that were able to repress replication of HCV replicon in hepatic cells [98].…”

Section: Aptamers For Virus Diagnosis and Treatmentmentioning

confidence: 99%

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

González

Martı́n

Fernández³

et al. 2016

Pharmaceuticals

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Appropriate diagnosis is the key factor for treatment of viral diseases. Time is the most important factor in rapidly developing and epidemiologically dangerous diseases, such as influenza, Ebola and SARS. Chronic viral diseases such as HIV-1 or HCV are asymptomatic or oligosymptomatic and the therapeutic success mainly depends on early detection of the infective agent. Over the last years, aptamer technology has been used in a wide range of diagnostic and therapeutic applications and, concretely, several strategies are currently being explored using aptamers against virus proteins. From a diagnostics point of view, aptamers are being designed as a bio-recognition element in diagnostic systems to detect viral proteins either in the blood (serum or plasma) or into infected cells. Another potential use of aptamers is for therapeutics of viral infections, interfering in the interaction between the virus and the host using aptamers targeting host-cell matrix receptors, or attacking the virus intracellularly, targeting proteins implicated in the viral replication cycle. In this paper, we review how aptamers working against viral proteins are discovered, with a focus on recent advances that improve the aptamers’ properties as a real tool for viral infection detection and treatment.

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“…Konno et al isolated RNA aptamers specific for HCV (−) IRES domain I. They showed that this aptamer inhibited up to 50% of the NS5B-mediated positive-strand RNA synthesis [40][41][42].…”

Section: Therapeutic Aptamers For Targeting the Hcv Viral Genomementioning

confidence: 99%

Aptamers for Anti-Viral Therapeutics and Diagnostics

Kim

Lee

2021

IJMS

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Viral infections cause a host of fatal diseases and seriously affect every form of life from bacteria to humans. Although most viral infections can receive appropriate treatment thereby limiting damage to life and livelihood with modern medicine and early diagnosis, new types of viral infections are continuously emerging that need to be properly and timely treated. As time is the most important factor in the progress of many deadly viral diseases, early detection becomes of paramount importance for effective treatment. Aptamers are small oligonucleotide molecules made by the systematic evolution of ligands by exponential enrichment (SELEX). Aptamers are characterized by being able to specifically bind to a target, much like antibodies. However, unlike antibodies, aptamers are easily synthesized, modified, and are able to target a wider range of substances, including proteins and carbohydrates. With these advantages in mind, many studies on aptamer-based viral diagnosis and treatments are currently in progress. The use of aptamers for viral diagnosis requires a system that recognizes the binding of viral molecules to aptamers in samples of blood, serum, plasma, or in virus-infected cells. From a therapeutic perspective, aptamers target viral particles or host cell receptors to prevent the interaction between the virus and host cells or target intracellular viral proteins to interrupt the life cycle of the virus within infected cells. In this paper, we review recent attempts to use aptamers for the diagnosis and treatment of various viral infections.

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An Rna Aptamer Containing Two Binding Sites Against the HCV Minus-Ires Domain I

Cited by 8 publications

References 33 publications

Aptamers in Diagnostics and Treatment of Viral Infections

Aptamers in Diagnostics and Treatment of Viral Infections

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

Aptamers for Anti-Viral Therapeutics and Diagnostics

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