An S-adenosyl Methionine Synthetase (SAMS) Gene from Andropogon virginicus L. Confers Aluminum Stress Tolerance and Facilitates Epigenetic Gene Regulation in Arabidopsis thaliana

Ezaki, Bunichi; Higashi, Aiko; Nanba, Norie; Nishiuchi, Takumi

doi:10.3389/fpls.2016.01627

Cited by 31 publications

(20 citation statements)

References 46 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Overexpression of the AvSAMS1 resulted in changes both in DNA and histone H3 methylation after plant exposure to Al. More interestingly, there were differences in the demethylation and methylation patterns at different positions in the promoter and coding regions of this gene (Ezaki et al, 2016).…”

Section: Epigenetic Mechanism Involved In Aluminum Toxicitymentioning

confidence: 89%

Epigenetic Control of Plant Response to Heavy Metal Stress: A New View on Aluminum Tolerance

et al. 2020

View full text Add to dashboard Cite

High concentrations of heavy metal (HM) ions impact agronomic staple crop production in acid soils (pH ≤ 5) due to their cytotoxic, genotoxic, and mutagenic effects. Among cytotoxic ions, the trivalent aluminum cation (Al3+) formed by solubilization of aluminum (Al) into acid soils, is one of the most abundant and toxic elements under acidic conditions. In recent years, several studies have elucidated the different signal transduction pathways involved in HM responses, identifying complementary genetic mechanisms conferring tolerance to plants. Although epigenetics has become more relevant in abiotic stress studies, epigenetic mechanisms underlying plant responses to HM stress remain poorly understood. This review describes the main epigenetic mechanisms related to crop responses during stress conditions, specifically, the molecular evidence showing how epigenetics is at the core of plant adaptation responses to HM ions. We highlight the epigenetic mechanisms that induce Al tolerance. Likewise, we analyze the pivotal relationship between epigenetic and genetic factors associated with HM tolerance. Finally, using rice as a study case, we performed a general analysis over previously whole-genome bisulfite-seq published data. Specific genes related to Al tolerance, measured in contrasting tolerant and susceptible rice varieties, exhibited differences in DNA methylation frequency. The differential methylation patterns could be associated with epigenetic regulation of rice responses to Al stress, highlighting the major role of epigenetics over specific abiotic stress responses.

show abstract

Section: Epigenetic Mechanism Involved In Aluminum Toxicitymentioning

confidence: 89%

Epigenetic Control of Plant Response to Heavy Metal Stress: A New View on Aluminum Tolerance

et al. 2020

View full text Add to dashboard Cite

show abstract

“…SAMS genes from multiple plant species have been validated to be involved in plant adaptation to multiple abiotic stresses. Overexpressing SAMS genes results in increased tolerance to drought (Kim et al, 2015), salinity (Kim et al, 2015; Ma et al, 2017), alkali (Gong et al, 2014), Al toxicity (Ezaki et al, 2016) and oxidative stresses (Ma et al, 2017). Like in MfSAMS1 transgenic tobacco plants with increased freezing and chilling tolerance (Guo et al, 2014), overexpression of MfSAMS1 resulted in enhanced chilling tolerance in transgenic stylo plants.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of MfSAMS1 Improves Chilling Tolerance in Transgenic Stylo

et al. 2019

View full text Add to dashboard Cite

Stylo [Stylosanthes guianensis (Aublet) Sw.] is an important forage legume and cover crop in tropical and southern subtropical regions. Its growth and survival are largely affected by low temperature in winter in subtropical regions. It is important to improve its chilling tolerance. S‐adenosylmethionine synthetase (SAMS) is the key enzyme catalyzing the formation of S‐adenosylmethionine (SAM), a precursor of polyamines synthesis. To improve chilling tolerance in stylo, MfSAMS1 from Medicago falcata was overexpressed in stylo plants. DNA gel blot hybridization and quantitative reverse transcription PCR analysis showed that MfSAMS1 was inserted into the genomes of transgenic plants and expressed. Compared to the wild‐type, transgenic plants had enhanced SAM and spermidine levels and increased chilling tolerance with higher level of the maximum photochemical efficiency of photosystem II (Fv/Fm) and lower ion leakage after chilling treatment. Polyamine oxidase activity and H2O2 levels were increased in transgenic plants compared with the wild‐type. In addition, higher activities of superoxide dismutase and catalase were observed in transgenic plants than in the wild‐type. The results suggest that overexpression of MfSAMS1 promoted polyamine synthesis and oxidation, which in turn improved H2O2–induced antioxidant protection and protected transgenic plants against chilling stress. Core Ideas Transgenic stylo plants overexpressing MfSAMS1 were produced. Plants showed promoted polyamine synthesis and oxidation. Transgenic plants had increased chilling tolerance with higher antioxidant levels.

show abstract

“…Ma et al (2003) found that the expression of the SAMS gene from Suaeda salsa is induced under NaCl stress (Ma et al 2003). Recently, it has been reported that the AvSAMS gene confers aluminum stress tolerance and facilitates epigenetic gene regulation (Ezaki et al 2016). SAMS is therefore known to play roles in various plant defense systems.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of S-Adenosylmethionine Synthetase Gene from Pyropia tenera Enhances Tolerance to Abiotic Stress

Hwang

Han

Hong

et al. 2017

Plant Breed. Biotech.

View full text Add to dashboard Cite

Pyropia tenera is an intertidal red alga of commercial significance owing to its popularity as a health-promoting seafood product. This alga grows in marine environments and is frequently exposed to high salinity and osmotic stress, which impact its growth. Therefore, the enhancement of stress tolerance in P. tenera is critical. In the present work, we aimed to elucidate the mechanisms underlying abiotic stress tolerance in this species; specifically, we identified the P. tenera S-adenosylmethionine synthetase-encoding gene (PtSAMS) and characterized its biological function. This gene, which is known to play a role in stress tolerance in other plants, was cloned and overexpressed in Escherichia coli under high-salinity conditions. The PtSAMS gene was found to encode a 385-amino-acid protein with a molecular weight of 41.8 kDa. In silico sequence alignment and phylogenetic analysis of the PtSAMS amino acid sequence showed that the encoded protein comprises three conserved domains and two motifs that are highly conserved in other plants. Growth assay results indicated that PtSAMS-overexpressing E. coli cells exhibit enhanced tolerance to salt stress. The results suggest that PtSAMS expression is induced by a combination of ion toxicity and osmotic stress resulting from exposure to high salinity in marine environments, and that this gene is expressed at housekeeping levels owing to growth in such conditions. The findings suggest that PtSAMS could be used as a potentially valuable bioresource with utility in the genetic engineering of salt stress-tolerant crop plants.

show abstract

An S-adenosyl Methionine Synthetase (SAMS) Gene from Andropogon virginicus L. Confers Aluminum Stress Tolerance and Facilitates Epigenetic Gene Regulation in Arabidopsis thaliana

Cited by 31 publications

References 46 publications

Epigenetic Control of Plant Response to Heavy Metal Stress: A New View on Aluminum Tolerance

Epigenetic Control of Plant Response to Heavy Metal Stress: A New View on Aluminum Tolerance

Overexpression of MfSAMS1 Improves Chilling Tolerance in Transgenic Stylo

Overexpression of S-Adenosylmethionine Synthetase Gene from Pyropia tenera Enhances Tolerance to Abiotic Stress

Contact Info

Product

Resources

About