An SI-traceable reference material for virus-like particles

Briones, Andrea; Martos, Gustavo; Bedu, Magali; Choteau, Tiphaine; Josephs, R D; Wielgosz, Robert; Ryadnov, Maxim G.

doi:10.1016/j.isci.2022.104294

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…An ability to release DNA during mitosis or target chromatin to anchor DNA in the forming nuclei can mitigate the factors impacting on the efficacy of DNA delivery. An ultimate solution may come from virus-like designs, whose development is reaching the stage of traceable reference materials to be used as benchmarks for gene delivery reagents . Unlike the peptide reagents used in this study, virus-like designs encapsulate nucleic acids rather than condense them.…”

Section: Discussionmentioning

confidence: 99%

“…An ultimate solution may come from virus-like designs, 52 whose development is reaching the stage of traceable reference materials to be used as benchmarks for gene delivery reagents. 53 Unlike the peptide reagents used in this study, virus-like designs encapsulate nucleic acids rather than condense them. Such systems can be made more responsive to cell targeting by capitalizing on the wealth of peptide surface chemistry and the virus architecture whose efficacy for functional gene delivery has yet to be matched.…”

Section: ■ Discussionmentioning

confidence: 99%

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Folding-Mediated DNA Delivery by α-Helical Amphipathic Peptides

Noble

Vila-Gómez

Rey

et al. 2023

ACS Biomater. Sci. Eng.

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The renaissance gene therapy experiences these days requires specialist biomaterials and a systemic understanding of major factors influencing their ability to deliver genetic material. Peptide transfection systems represent a major class of such biomaterials. Several peptidic reagents have been commercialized to date. However, a comparative assessment of peptide sequences alone without auxiliary support or excipients against a common determinant for their ability to complex and deliver DNA has been lacking. This study cross-compares commercial and experimental transfection reagents from the same family of helical amphiphiles. Factors defining the efficacy of DNA delivery including cell uptake and gene expression are assessed along with cytotoxicity and DNA complexation. The results show that despite differences in sequence composition, length, and origin, peptide reagents of the same structural family exhibit similar characteristics and limitations with common variability trends. The cross-comparison revealed that functional DNA delivery is independent of the peptide sequence used but is mediated by the ability of the reagents to co-fold with DNA. Peptide folding proved to be the common determinant for DNA complexation and delivery by peptidic transfection reagents.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Folding-Mediated DNA Delivery by α-Helical Amphipathic Peptides

Noble

Vila-Gómez

Rey

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…As part of the programmes related to Metrology for Health and diagnostic markers, the final report of part II of the CCQM SARS-CoV-2 antibody quantification pilot study, CCQM-P216, coordinated by NIM (China), with the BIPM and NRC (Canada), was published [53]. A CITAC best paper award was received for the NPL and BIPM paper on compositional analysis of C3-triskelion in iScience [54]. An on-site hybrid meeting was held at the BIPM headquarters in February 2023, with representatives from NRC (Canada), NIBSC (UK), NIM (China), LNE (France), CDC (USA) and the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) to plan for the parathyroid hormone (PTH 1-84) comparison and use of such material in calibration hierarchies for the measurement of PTH (1-84) in serum or blood.…”

Section: Chemical Metrologymentioning

confidence: 99%

News from the BIPM laboratories—2023

Stock,

Tavella,

Gressier

et al. 2024

Metrologia

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In order to fulfil its mission to ensure and promote the global comparability of measurements, the BIPM operates laboratories in the fields of physical metrology, time, ionizing radiation and chemistry. These laboratories act as centres for scientific and technical collaboration between Member States providing capabilities for international measurement comparisons on a shared cost basis. They coordinate international comparisons of national measurement standards agreed to be of the highest priority, and they establish and maintain appropriate reference standards for use as the basis of key international comparisons at the highest level and provide selected calibrations from them.In the following sections, we provide highlights of the work the laboratories have undertaken during 2023.

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“…The NPL and BIPM published a paper on compositional analysis of C3-triskelion in iScience [40] as part of the programmes related to Metrology for Health and diagnostic markers, as well as the final reports of CCQM-K115.c [41] and P55.2.c [42] (HbA1c hexapeptide (GE) purity) CCQM-K115.2018 [43] and P55.2.2018 [44] (HbA0 hexapeptide (VE) purity). Work to complete the final report part II of the CCQM SARS-CoV-2 antibody quantification pilot study, CCQM-P216, coordinated by NIM (China), with BIPM and NRC (Canada), was progressed.…”

Section: Chemical Metrologymentioning

confidence: 99%

News from the BIPM laboratories—2022

et al. 2023

View full text Add to dashboard Cite

In order to fulfil its mission to ensure and promote the global comparability of measurements, the BIPM operates laboratories in the fields of physical metrology, time, ionizing radiation and chemistry. These laboratories act as centres for scientific and technical collaboration between Member States providing capabilities for international measurement comparisons on a shared cost basis. They coordinate international comparisons of national measurement standards agreed to be of the highest priority, and they establish and maintain appropriate reference standards for use as the basis of key international comparisons at the highest level and provide selected calibrations from them. In the following sections, we provide highlights of the work the laboratories have undertaken during 2022.

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An SI-traceable reference material for virus-like particles

Cited by 9 publications

References 41 publications

Folding-Mediated DNA Delivery by α-Helical Amphipathic Peptides

Folding-Mediated DNA Delivery by α-Helical Amphipathic Peptides

News from the BIPM laboratories—2023

News from the BIPM laboratories—2022

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