An sRNA Screen for Reversal of Quinolone Resistance in <i>Escherichia coli</i>

Bhatnagar, Kamya; Hinz, Aaron; Kohlman, Melissa; Wong, Alex

doi:10.1534/g3.119.400199

Cited by 12 publications

(19 citation statements)

References 93 publications

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“…ESBLs (extended-spectrum β-Lactamases) and cabapenemases production are the two main mechanisms for the development of resistance in Klebsiella pneumoniae [ 104 ]. Ciprofloxacin resistance to Klebsiella pneumoniae became resistant through the modification of the gyrA gene after the mutation could be reduced by knocking out the following genes: recA, xseA, fis, recC, and tolC [ 105 ]. From the results, it was concluded that the plant extracts are not effective for redressal of the respective antibiotic resistance in bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Levofloxacin is resistant to Klebsiella pneumoniae , and became resistant to the modification of the gyrA gene after the mutation was reduced by knocking out the following genes: recA, xseA, fis, recC, and tolC [ 105 ]. From the results, it was concluded that the plant extracts were not effective for redressal of the respective antibiotic resistance in bacteria.…”

Section: Resultsmentioning

confidence: 99%

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Achyranthes aspera Extracts as Adjuvants for the Redressal of Antibiotic Resistance

et al. 2022

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Achyranthes aspera seeds and leaves are believed to reverse antibiotic resistance and increase the efficacy of current drugs. Achyranthes aspera seeds and leaves contain many secondary metabolites needed for the redressal of antibiotic resistance. In the present study, seven different antibiotics were used against five different strains of bacteria such as Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. For Methicillin-resistant Staphylococcus aureus Cefoxitin, Penicillin, and Co-trimoxazole were resistant out of seven antibiotics. The zone of inhibition for all these three antibiotics goes from the resistant to the sensitive range after the combination with plant extracts. For Enterococcus faecalis, Ciprofloxacin, Levofloxacin, Penicillin, Amoxicillin, Imipenem, and Vancomycin were resistant after treatment with the plant extracts, and the Ciprofloxacin, Levofloxacin, Imipenem, and Vancomycin zones of inhibition were from the resistant to the sensitive range. An increase in zone sizes was observed for Penicillin, but it remained resistant while no zone of inhibition was observed for Amoxicillin. For Acinetobacter baumannii, Ciprofloxacin, Levofloxacin, Ceftriaxone, Ceftazidime, and Imipenem were resistant. After a combination of these antibiotics with plant extracts, a change in zone sizes was observed for Levofloxacin and Ceftriaxone, but it was not considerable as it remained in the resistance and intermediate ranges. No zones of inhibition were observed for Ciprofloxacin, Ceftazidime, or Imipenem. For Klebsiella pneumoniae, all the antibiotics were resistant. An increase in zone sizes was observed after a combination with plant extracts for Ceftazidime and Imipenem in Klebsiella pneumoniae, but it remained in the resistance category. No zone of inhibition was observed for Pseudomonas aeruginosa before or after using plant extracts against any antibiotic. This study suggests that the Achyranthes aspera seed and leaf extracts can reverse antibiotic resistance without any side effects on the human body, and that they can reverse antibiotic resistance naturally.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Achyranthes aspera Extracts as Adjuvants for the Redressal of Antibiotic Resistance

et al. 2022

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“…S1). Vectors elements supporting conjugative transfer (oriT) (23) and counterselection (sacB) (37) can be exploited in mobilizable and curable E. coli vectors (38). For microbial species that do not support pMB1based plasmid replication, the vectors can be utilized for chromosomal allelic replacement (sacB vectors) (26,39) or site-specific transposition (mini-Tn7 vectors) (25,40,27).…”

Section: Discussionmentioning

confidence: 99%

Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters

Hinz

Stenzler

Poulain

2021

Preprint

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Microbial bioreporters provide direct insight into cellular processes by producing a quantifiable signal dictated by reporter gene expression. The core of a bioreporter is a genetic circuit in which a reporter gene (or operon) is fused to promoter and regulatory sequences that govern its expression. In this study, we develop a system for constructing novel Escherichia coli bioreporters based on Golden Gate assembly, a synthetic biology approach for the rapid and seamless fusion of DNA fragments. Gene circuits are generated by fusing promoter and reporter sequences encoding yellow fluorescent protein, mCherry, bacterial luciferase, and an anaerobically active flavin-based fluorescent protein. We address a barrier to the implementation of Golden Gate assembly by designing a series of compatible destination vectors that can accommodate the assemblies. We validate the approach by measuring the activity of constitutive bioreporters and mercury and arsenic biosensors in quantitative exposure assays. We also demonstrate anaerobic quantification of mercury and arsenic in biosensors that produce flavin-based fluorescent protein, highlighting the expanding range of redox conditions that can be examined by microbial bioreporters.

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“…The authors found that suppression of the SOS response, as a consequence of recA deletion, increased the bactericidal activity of ciprofloxacin and reduced survival in resistant strains, providing evidence that suppression of the SOS pathway can reverse FQs resistance [ 234 ]. Recently, sRNA screening identified candidate genes whose inactivation restored susceptibility to ciprofloxacin in strains with a gyrA S83L substitution that confers a high level of resistance in E. coli [ 235 ]. On the other hand, investigations with palmatine, an isoquinolone alkaloid, extracted from plants, showed resensitization of E. coli strains with plasmids harboring qnrS and aac(6′)-lb-cr .…”

Section: Mechanisms Of Resistance To Quinolonesmentioning

confidence: 99%

Biological Effects of Quinolones: A Family of Broad-Spectrum Antimicrobial Agents

et al. 2021

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Broad antibacterial spectrum, high oral bioavailability and excellent tissue penetration combined with safety and few, yet rare, unwanted effects, have made the quinolones class of antimicrobials one of the most used in inpatients and outpatients. Initially discovered during the search for improved chloroquine-derivative molecules with increased anti-malarial activity, today the quinolones, intended as antimicrobials, comprehend four generations that progressively have been extending antimicrobial spectrum and clinical use. The quinolone class of antimicrobials exerts its antimicrobial actions through inhibiting DNA gyrase and Topoisomerase IV that in turn inhibits synthesis of DNA and RNA. Good distribution through different tissues and organs to treat Gram-positive and Gram-negative bacteria have made quinolones a good choice to treat disease in both humans and animals. The extensive use of quinolones, in both human health and in the veterinary field, has induced a rise of resistance and menace with leaving the quinolones family ineffective to treat infections. This review revises the evolution of quinolones structures, biological activity, and the clinical importance of this evolving family. Next, updated information regarding the mechanism of antimicrobial activity is revised. The veterinary use of quinolones in animal productions is also considered for its environmental role in spreading resistance. Finally, considerations for the use of quinolones in human and veterinary medicine are discussed.

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An sRNA Screen for Reversal of Quinolone Resistance in Escherichia coli

Cited by 12 publications

References 93 publications

Achyranthes aspera Extracts as Adjuvants for the Redressal of Antibiotic Resistance

Achyranthes aspera Extracts as Adjuvants for the Redressal of Antibiotic Resistance

Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters

Biological Effects of Quinolones: A Family of Broad-Spectrum Antimicrobial Agents

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