An ultra-sensitive dual-responsive aptasensor with combination of liquid crystal and intercalating dye molecules: A food toxin case study

Khoshbin, Zahra; Abnous, Khalil; Taghdisi, Seyed Mohammad; Verdian, Asma; Sameiyan, Elham; Ramezani, Mohammad; Alibolandi, Mona

doi:10.1016/j.foodchem.2022.132265

Cited by 23 publications

(3 citation statements)

References 37 publications

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“…For these reasons, aptamers are used in many analytical fields, such as diagnosis, drug and blood analysis. [41][42][43][44][45] The high affinity for the target analyte leads to high stability of the complex aptamer-analyte, low detection limits, 46,47 and in some cases, the possibility of reusing the sensor. 48 The development of the microfluidic devices allows for the integration of aptamers into a microfluidic chip, thus obtaining innovative sensor devices (lab-onchip).…”

Section: Aptamer Sensorsmentioning

confidence: 99%

Cortisol sensing by optical sensors

Santonocito,

Puglisi,

Cavallaro

et al. 2024

Analyst

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Section: Aptamer Sensorsmentioning

confidence: 99%

Cortisol sensing by optical sensors

Santonocito,

Puglisi,

Cavallaro

et al. 2024

Analyst

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“…[31,32] A number of intercalating dyes have been developed for the rapid detection of bacterial and viral pathogens using LAMP methods. [33,34] These dyes can achieve a minimum of 10 copies of DNA or 10 CFU/reaction as detection limits, and the fluorescent signal can be readily detected within 10 min. [35] Nevertheless, UV illumination is still required for fluorescent readout.…”

Section: Introductionmentioning

confidence: 99%

Pipette‐free field‐deployable molecular diagnostic kit for bimodal visual detection of infectious RNA viruses

Park,

Trinh,

Song

et al. 2024

Biotechnology Journal

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Here, we developed a field‐deployable molecular diagnostic kit for the detection of RNA viruses that operates in a pipette‐free manner. The kit is composed of acrylic sticks, PCR tubes, and palm‐sized three‐dimensional(3D)‐printed heaters operated by batteries. The kit performs RNA extraction, reverse transcriptase loop‐mediated isothermal amplification (RT‐LAMP), and visual detection in one kit. An acrylic stick was engraved with one shallow and one deep cylindrical chamber at each end for the insertion of an FTA card and ethidium homodimer‐1 (EthD‐1), respectively, to perform RNA extraction/purification and bimodal visual detection of the target amplicons. First, an intercalation of EthD‐1 into the target DNA initially produces fluorescence upon UV illumination. Next, the addition of a strong oxidant, in this case sodium (meta) periodate (NaIO4), produces intense aggregates in the presence of EthD‐1‐intercalated DNA, realized by electrostatic interaction. In the absence of the target amplicon, no fluorescence or aggregates are observed. Using this kit, two major infectious viruses—severe fever with thrombocytopenia syndrome virus (SFTSV) and severe acute respiratory syndrome coronavirus (SARS‐CoV‐2)—were successfully detected in 1 h, and the limits of detection (LOD) were approximately 1 virus μL−1 for SFTSV and 103 copies μL−1 for SARS‐CoV‐2 RNA. The introduced kit is portable, end‐user‐friendly, and can be operated in a pipette‐free manner, paving the way for simple and convenient virus detection in resource‐limited settings.

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“…Liquid crystals (LCs) exhibit unique characterstics such as birefringence and a long-range orientation order, enabling translating the molecular events into optical signals under crossed polarizers. − Different types of LC-based sensors have been developed and extensively applied in detecting various analytes such as enzymes, proteins, , nucleic acids, small molecules, , and environmental pollutants. − In particular, the LC-based sensors obtained via directly observing the surfactant adsorption/desorption behavior at the aqueous/LC interface are promising for commercialization because different analytes can be detected via the surfactant-mediated chemical and biological events. − With a polarized optical microscope (POM), bright or dark LC images can be observed and the images can be analyzed for quantitative detection. There are still some challenges to further developing the “interfacial type” LC-based sensors.…”

mentioning

confidence: 99%

Construction of Liquid Crystal-Based Sensors Using Enzyme-Linked Dual-Functional Nucleic Acid on Magnetic Beads

Song,

Khan,

et al. 2023

Anal. Chem.

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The development of liquid crystal (LC)-based sensors with superior performances such as high portability, excellent stability, great convenience, and remarkable sensitivity is highly demanded. This work proposes a new strategy for constructing the LC-based sensor using enzyme-linked dualfunctional nucleic acid (d-FNA) on magnetic beads (MBs). The detection of kanamycin (KA) is demonstrated as a model. Acetylcholinesterase (AChE) is assembled onto the KA aptamermodified MBs with a d-FNA strand that consists of an AChE aptamer and the complementary sequence of a KA aptamer. As the specific recognition of KA by its aptamer triggers the release of AChE from the MBs, the myristoylcholine (Myr) solution after incubation with the MBs causes the black image of the LCs due to the formation of the Myr monolayer at the aqueous/LC interface. Otherwise, in the absence of KA, AChE is still decorated on the MBs and causes the hydrolysis of Myr. Therefore, a bright image of LCs is obtained. The detection of KA is successfully achieved with a lower detection limit of 48.1 pg/mL. In addition, a thin polydimethylsiloxane (PDMS) layer-coated glass and a portable optical device are used to improve the stability and portability of the LC-based sensor to advance potential commercial applications. Furthermore, the detection of KA in milk with a portable device is demonstrated, showing the potential of the proposed enzymelinked LC-based sensor.

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An ultra-sensitive dual-responsive aptasensor with combination of liquid crystal and intercalating dye molecules: A food toxin case study

Cited by 23 publications

References 37 publications

Cortisol sensing by optical sensors

Cortisol sensing by optical sensors

Pipette‐free field‐deployable molecular diagnostic kit for bimodal visual detection of infectious RNA viruses

Construction of Liquid Crystal-Based Sensors Using Enzyme-Linked Dual-Functional Nucleic Acid on Magnetic Beads

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