An Ultra‐sensitive Electrochemiluminescent Detection of Carcinoembryonic Antigen Using a Hollowed‐out Electrode

Liu, Gen; Gao, Hui; Chen, Jiajia; Shao, Congying; Chen, Feifei

doi:10.1002/elan.202060624

Cited by 4 publications

(1 citation statement)

References 39 publications

(43 reference statements)

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“…Immunoassay is the most commonly used strategy for CEA determination that detects the signal changes caused by the binding of CEA and specific antibodies. Depending on the signal, methods including the enzyme-linked immunosorbent assay (ELISA) [ 6 ], radioimmunoassay (RIA), chemiluminescence immunoassay (CLIA) [ 7 ], electrochemical immunoassay [ 8 , 9 , 10 , 11 ], and electrochemiluminescence (ECL) immunoassay [ 12 , 13 ] have been developed. However, an ELISA usually requires a longer detection time and may be influenced by interfering factors.…”

Section: Introductionmentioning

confidence: 99%

Immunosensor with Enhanced Electrochemiluminescence Signal Using Platinum Nanoparticles Confined within Nanochannels for Highly Sensitive Detection of Carcinoembryonic Antigen

Zhang,

et al. 2023

Molecules

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Rapid, highly sensitive, and accurate detection of tumor biomarkers in serum is of great significance in cancer screening, early diagnosis, and postoperative monitoring. In this study, an electrochemiluminescence (ECL) immunosensing platform was constructed by enhancing the ECL signal through in situ growth of platinum nanoparticles (PtNPs) in a nanochannel array, which can achieve highly sensitive detection of the tumor marker carcinoembryonic antigen (CEA). An inexpensive and readily available indium tin oxide (ITO) glass electrode was used as the supporting electrode, and a layer of amino-functionalized vertically ordered mesoporous silica film (NH2-VMSF) was grown on its surface using an electrochemically assisted self-assembly method (EASA). The amino groups within the nanochannels served as anchoring sites for the one-step electrodeposition of PtNPs, taking advantage of the confinement effect of the ultrasmall nanochannels. After the amino groups on the outer surface of NH2-VMSF were derivatized with aldehyde groups, specific recognition antibodies were covalently immobilized followed by blocking nonspecific binding sites to create an immunorecognition interface. The PtNPs, acting as nanocatalysts, catalyzed the generation of reactive oxygen species (ROS) with hydrogen peroxide (H2O2), significantly enhancing the ECL signal of the luminol. The ECL signal exhibited high stability during continuous electrochemical scanning. When the CEA specifically bound to the immunorecognition interface, the resulting immune complexes restricted the diffusion of the ECL emitters and co-reactants towards the electrode, leading to a reduction in the ECL signal. Based on this immune recognition-induced signal-gating effect, the immunosensor enabled ECL detection of CEA with a linear range of 0.1 pg mL−1 to 1000 ng mL−1 with a low limit of detection (LOD, 0.03 pg mL−1). The constructed immunosensor demonstrated excellent selectivity and can achieve CEA detection in serum.

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