An ultra-sensitive uric acid second generation biosensor based on chemical immobilization of uricase on functionalized multiwall carbon nanotube grafted palm oil fiber in the presence of a ferrocene mediator

Abstract

In this work, Palm oil fiber (POF) grafted functionalized multiwall carbon nanotube (FMWCNT) decorated ferrocene (Fc) have been drop coated on platinum electrode (Pt), in which uricase (UOx) has been...

Preparation and Characterization of Alkali Activated Carbon Based Theobroma Cocoa Pods: Application for Electrochemical Determination of Xanthine in Fresh Fish Sample

“…For the electroanalysis of xanthine, the bare glassy carbon electrode (GCE) was cleaned for each measurement with alumina powder, rinsed with distilled water and finally sonicated with a 1/1 ethanol: H 2 O (v/v), before been modified with a suspension of RCP and ACCP. [14] The dispersion solutions of RCP and ACCP were prepared by dispersing 5 mg of RCP and ACCP in 1 mL of distilled water with ultrasound for 10 min. 3 μL of each suspension were drop coated on the shining surface of bare GCE (geometry surface = 0.071 cm 2 ), then left to dry in an oven for 10 min at 50 °C.…”

“…[3,4] Biomass materials have been widely used as electrode materials for the modification of conventional working electrodes. The resulting modified electrodes were used for the sensing of compounds such as dyes, [5,6] pesticides, [7,8] phenolic, [9,10] heavy metals [11,12] constituent of pathological samples, [13,14] and food additives. [9,15] The ultimate and long term goal of the majority of such research is to develop miniaturized sensor devices for field deployment and point of care testing (POCT).…”

“…[28] In this way, the electroanalysis of xanthine has been done by many researchers, [29][30][31][32] but they used enzyme which had a number of problem like lack of stability, impossible reusability, slow electron transfer. The design of a biosensor is sometime very complex [14,33] because non-covalent immobilization may lead to leakage of electrons enzymes, which remain to be improved.…”

Evaluation of two core–shell (Ag2S@- and Bi2S3@-) sensors based on a metal–organic framework (NH2-MIL-125-Ti)/polyaniline for the electroanalysis of uric acid in urine samples

“…19 Moreover, in physiological samples, uric acid is present at concentrations ranging from 160 to 500 mM. [20][21][22] Therefore, the challenge while developing electrochemical sensors for this species is to prepare a receptor selective for the target analyte and which can detect it at a concentration in the physiological range or even less than that. Electrochemical sensors based on modification of a glassy carbon electrode (GCE) with different graphene-based composite materials show promising results in overcoming this problem.…”

“…31 In our previous works, we have developed UA sensors and biosensors, based on biomass materials such as egg shell nano-CaCO 3 and palm oil fibers. [22][23][24][25][26][27][28][29][30][31][32] The design of new sensing materials for the detection of this physiological compound with higher sensitivity and resolution remains an area requiring much more work as the strategies for the development of wearable sensors are the final goal. 33 Metal-organic frameworks (MOFs) as a new class of electrode materials offering new hope in this regard due to the synergistic catalytic behaviour and the electroactivity of the incorporated metals in MOFs.…”