An ultra-specific and sensitive sandwich ELISA for imatinib using two anti-imatinib antibodies

Saita, Tetsuya; Hosoya, Kazuhisa; Yamamoto, Yuta; Kimura, Sakiko; Narisawa, Yutaka; Shin, Masashi

doi:10.1016/j.aca.2017.03.034

Cited by 26 publications

(12 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are shown in Table 3. The coefficient of variation of each sample was less than 8%, which indicated that the established sandwich ELISA method had good intragroup and good intergroup repeatability [54]. We evaluated the stability of the probes as described [39,55].…”

Section: Repeatability and Stability Testmentioning

confidence: 99%

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Yuqin

Zhao

et al. 2020

IJERPH

View full text Add to dashboard Cite

As humans and climate change continue to alter the landscape, novel disease risk scenarios have emerged. Sever fever with thrombocytopenia syndrome (SFTS), an emerging tick-borne infectious disease first discovered in rural areas of central China in 2009, is caused by a novel bunyavirus (SFTSV). The potential for SFTS to spread to other countries in combination with its high fatality rate, possible human-to-human transmission, and extensive prevalence among residents and domesticated animals in endemic regions make the disease a severe threat to public health. Because of the lack of preventive vaccines or useful antiviral drugs, diagnosis of SFTS is the key to prevention and control of the SFTSV infection. The development of serological detection methods will greatly improve our understanding of SFTSV ecology and host tropism. We describe a highly sensitive protein detection method based on gold nanoparticles (AuNPs) and enzyme-linked immunosorbent assay (ELISA)—AuNP-based ELISA. The optical sensitivity enhancement of this method is due to the high loading efficiency of AuNPs to McAb. This enhances the concentration of the HRP enzyme in each immune sandwich structure. The detection limit of this method to the nucleocapsid protein (NP) of SFTSV was 0.9 pg mL−1 with good specificity and reproducibility. The sensitivity of AuNP-based ELISA was higher than that of traditional ELISA and was comparable to real-time quantitative polymerase chain reaction (qRT-PCR). The probes are stable for 120 days at 4 °C. This can be applied to diagnosis and hopefully can be developed into a commercial ELISA kit. The ultrasensitive detection of SFTSV will increase our understanding of the distribution and spread of SFTSV, thus helping to monitor the changes in tick-borne pathogen SFTSV risk in the environment.

show abstract

Section: Repeatability and Stability Testmentioning

confidence: 99%

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Yuqin

Zhao

et al. 2020

IJERPH

View full text Add to dashboard Cite

show abstract

“…The reason for this is that it is very challenging to produce an anti-erlotinib antibody that is not cross-reactive with O -desmethyl erlotinib, the major metabolite of erlotinib. We previously created a specific antibody, which is not cross-reactive with the major metabolite, and changed part of the structure of the drug to a hapten antigenic structure, by considering the structure of the major metabolite, to develop an enzyme immunoassay technique that could be used for pharmacokinetic studies of the antibody [18] , [19] , [20] . Similarly, we created a specific anti-erlotinib antibody by changing part of the structure of erlotinib to a hapten antigenic structure to develop enzyme immunoassay techniques that could be used for pharmacokinetic studies on erlotinib.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent assay

Yamamoto

Saita

Shin

2018

Journal of Pharmaceutical Analysis

Self Cite

View full text Add to dashboard Cite

A selective and sensitive competitive enzyme-linked immunosorbent assay (ELISA) method was developed and validated for the quantification of erlotinib in 50 µL of samples of human serum. Anti-erlotinib serum was obtained by immunizing mice with an antigen conjugated with bovine serum albumin and 3,4-bis(2-methoxyethoxy)benzoic acid using the N-succinimidyl ester method. Enzyme labeling of erlotinib with horseradish peroxidase was similarly performed using 3,4-bis(2-methoxyethoxy)benzoic acid. A simple competitive ELISA for erlotinib was developed using the principle of direct competition between erlotinib and the enzyme marker for anti-erlotinib antibody, which had been immobilized on the plastic surface of a microtiter plate. Serum erlotinib concentrations lower than 40 ng/mL were reproducibly measurable using the ELISA. This ELISA was specific to erlotinib and showed very slight cross-reactivity (6.7%) with a major metabolite, O-desmethyl erlotinib. Using this assay, drug levels were easily measured in the blood of mice after oral administration of erlotinib at a single dose of 30 mg/kg. ELISA should be used as a valuable tool for therapeutic drug monitoring and in pharmacokinetic studies of erlotinib.

show abstract

“…It is important to detect disease biomarkers for early diagnosis, prognosis tracking and therapeutic evaluation. Numerous methods have been reported to detect target proteins, such as enzyme-linked immunosorbent assay (ELISA), electrochemical immunology sensors and mass spectra analysis [ 7 , 8 , 9 , 10 , 11 , 12 ]. However, these methods have some limitations; for example, most involve multistep and time-consuming procedures and often cannot be well applied to intracellular detection.…”

Section: Introductionmentioning

confidence: 99%

A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins

Shi

Zhang

Liu

et al. 2018

Sensors

View full text Add to dashboard Cite

The detection of proteins is of great biological significance as disease biomarkers in early diagnosis, prognosis tracking and therapeutic evaluation. Thus, we developed a simple, sensitive and universal protein-sensing platform based on peptide and graphene oxide (GO). The design consists of a fluorophore (TAMRA, TAM), a peptide containing eight arginines and peptide ligand that could recognize the target protein, and GO used as a quencher. To demonstrate the feasible use of the sensor for target detection, Bcl-xL was evaluated as the model target. The sensor was proved to be sensitive and applied for the detection of the target proteins in buffer, 2% serum and living cells.

show abstract

An ultra-specific and sensitive sandwich ELISA for imatinib using two anti-imatinib antibodies

Cited by 26 publications

References 18 publications

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Enzyme-Antibody-Modified Gold Nanoparticle Probes for the Ultrasensitive Detection of Nucleocapsid Protein in SFTSV

Quantitative determination of erlotinib in human serum using competitive enzyme-linked immunosorbent assay

A New Strategy Involving the Use of Peptides and Graphene Oxide for Fluorescence Turn-on Detection of Proteins

Contact Info

Product

Resources

About