An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury

Sun, Jui Sheng; Tsuang, Yang Hwei; Chen, I‐Jen; Huang, Wan-Ching; Hang, Yi‐Shiong; Lu, Fung‐Jou

doi:10.1016/s0305-4179(97)00115-0

Cited by 144 publications

(81 citation statements)

References 20 publications

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“…Although the source of this false signal is not yet fully understood, current evidence from preclinical experiments points toward long-lived, thermally induced chemiluminescence (25). Because the emission seems to be related to temperature, which can reach up to 250°C at the tip of the diathermy device, electrosurgical devices that operate at much lower temperatures are currently being tested (26).…”

Section: Discussionmentioning

confidence: 99%

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

et al. 2016

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In early-stage breast cancer, the primary treatment option for most women is breast-conserving surgery (BCS). There is a clear need for more accurate techniques to assess resection margins intraoperatively, because on average 20% of patients require further surgery to achieve clear margins. Cerenkov luminescence imaging (CLI) combines optical and molecular imaging by detecting light emitted by 18 F-FDG. Its high-resolution and small size imaging equipment make CLI a promising technology for intraoperative margin assessment. A first-in-human study was conducted to evaluate the feasibility of 18 F-FDG CLI for intraoperative assessment of tumor margins in BCS. Methods: Twenty-two patients with invasive breast cancer received 18 F-FDG (5 MBq/kg) 45-60 min before surgery. Sentinel lymph node biopsy was performed using an increased 99m Tc-nanocolloid activity of 150 MBq to facilitate nodal detection against the g-probe background signal (cross-talk) from 18 F-FDG. The cross-talk and 99m Tc dose required was evaluated in 2 lead-in studies. Immediately after excision, specimens were imaged intraoperatively in an investigational CLI system. The first 10 patients were used to optimize the imaging protocol; the remaining 12 patients were included in the analysis dataset. Cerenkov luminescence images from incised BCS specimens were analyzed postoperatively by 2 surgeons blinded to the histopathology results, and mean radiance and margin distance were measured. The agreement between margin distance on CLI and histopathology was assessed. Radiation doses to staff were measured. Results: Ten of the 12 patients had an elevated tumor radiance on CLI. Mean radiance and tumor-to-background ratio were 560 6 160 photons/s/cm 2 /sr and 2.41 6 0.54, respectively. All 15 assessable margins were clear on CLI and histopathology. The agreement in margin distance and interrater agreement was good (k 5 0.81 and 0.912, respectively). Sentinel lymph nodes were successfully detected in all patients. The radiation dose to staff was low; surgeons received a mean dose of 34 6 15 mSv per procedure. Conclusion: Intraoperative 18 F-FDG CLI is a promising, low-risk technique for intraoperative assessment of tumor margins in BCS. A randomized controlled trial will evaluate the impact of this technique on reexcision rates. In early-stage breast cancer, the primary treatment option for most women is breast-conserving surgery (BCS) by wide local excision (WLE) of the tumor. WLE often fails to achieve clear surgical margins, and on average 20% of patients who undergo BCS will require repeated surgery to achieve clear margins (1) (although this may vary because there is no global agreement of the definition of clear margins). Reoperations potentially have several negative consequences including delayed commencement of adjuvant therapy, worse cosmesis, increased patient anxiety, and costs (2,3).There have been several attempts to assess surgical margins intraoperatively to reduce breast cancer reoperation rates after WLE (1). Techniques evaluated to date in...

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Section: Discussionmentioning

confidence: 99%

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

et al. 2016

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“…The total proanthocyanidin was determined using the procedure reported by Sun et al (1998). A volume of 0.5 mL of 0.1 mg/mL of extract solution was mixed with 3.0 mL of 4% vanillin-methanol solution and 1.5 mL hydrochloric acid; the mixture was allowed to stand for 15 min at room temperature, the absorbance was measured at 500 nm.…”

Section: Determination Of Proanthocyanidin Contentmentioning

confidence: 99%

In vitro assessment of antioxidant, phytochemical and nutritional properties of extracts from the leaves of Ocimum Gratissimum (linn).

Igbinosa¹,

Uzunuigbe²,

Igbinosa³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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The antioxidant, phytochemical and nutritional properties of acetone, methanol and aqueous extracts of the leaves of Ocimum gratissimum (Linn) were investigated to evaluate the therapeutic and nutritional potential of the leaves of this plant. The antioxidant of the plant extracts were assessed against 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing agent. Total phenolics, flavonoids, flavonols and proanthocyanidins were determined to assess their corresponding effect on the antioxidant activity of this plant. The extracts exhibited DPPH and ABTS .+ radical scavenging activities, which was comparable to butylated hydroxytoluene (BHT). The phytochemical screening revealed the presence of alkaloids, tannins, saponin, steroids, cardiacglycoside, flavonoid, terpenoids and phenol. The proximate analysis confirms that the leaves contain appreciable amount of ash, crude protein, lipids, fibre and carbohydrates. The macro and micro elements and constituents revealed that the leaves contain significant amount of sodium, potassium, calcium, magnesium, iron, zinc, phosphorus, copper, nitrogen, and manganese. This study shows that the leaf can be used as a therapeutic agent and justifies its application in folkloric medicine.

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“…Determination of the total proanthocyanidins was based on the procedure of Sun et al (1998). A volume of 0.5 mL of 1 mg/mL extract solution was mixed with 3 mL of 4% vanillin-methanol solution and 1.5 mL hydrochloric acid.…”

Section: Determination Of Total Proanthocyanidins Contentmentioning

confidence: 99%

Anti-oxidant and anti-acetylcholinesterase activities of Zygophyllum album

Kchaou

Salah

Mhiri

et al. 2015

Bangladesh J Pharmacol

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The present study was carried out to explore the anti-acetylcholinesterase activity and anti-oxidant effect as well as content of phenolic compounds of various extracts from Zygophyllum album. It was observed that dichloromethane and ethanol extracts were endowed with a powerful anti-acetylcholinesterase activity with IC50 values of 40 and 58 µg/mL, respectively. These two extracts exhibited the highest DPPH radical scavenging activity (IC50=0.2 mg/mL), the highest total anti-oxidant capacity (185.2 and 222.4 mg vitamin E/mg extract, respectively), the highest reducing power effect, and the highest β-carotene bleaching capacity. The findings showed that the extents of anti-acetylcholinesterase activity of Z. album extracts as well as their anti-oxidant capacity are in accordance with their phenolic contents. Hence, Z. album leaves would be useful against Alzheimer's disease. VIDEO CLIP<a href="https://www.youtube.com/v/M03zi4Mzh6k">Determination of flavonoids content:</a> 2 min 50 sec

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An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury

Cited by 144 publications

References 20 publications

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

<i>In vitro</i> assessment of antioxidant, phytochemical and nutritional properties of extracts from the leaves of <i>Ocimum Gratissimum</i> (linn).

Anti-oxidant and anti-acetylcholinesterase activities of <i>Zygophyllum album</i>

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An ultra-weak chemiluminescence study on oxidative stress in rabbits following acute thermal injury

Cited by 144 publications

References 20 publications

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using 18F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using 18F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

<i>In vitro</i> assessment of antioxidant, phytochemical and nutritional properties of extracts from the leaves of <i>Ocimum Gratissimum</i> (linn).

Anti-oxidant and anti-acetylcholinesterase activities of <i>Zygophyllum album</i>

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Product

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Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study

Intraoperative Assessment of Tumor Resection Margins in Breast-Conserving Surgery Using ¹⁸F-FDG Cerenkov Luminescence Imaging: A First-in-Human Feasibility Study