An ultralow background substrate for protein microarray technology

Feng, Hui; Zhang, Qingyang; Ma, Hongwei; Zheng, Bo

doi:10.1039/c5an00852b

Cited by 16 publications

(21 citation statements)

References 31 publications

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“…Uniform PDA patterns with microstructures less than 10 µm were successfully fabricated on the CYTOP surface. By contrast, conventional microcontact printing failed to fabricate PDA microstructures with featured length less than 100 µm . In conventional microcontact printing, the surface energy properties of the PDMS stamp and the hydrophobic substrate were similar, and both the PDMS stamp and the hydrophobic substrate have a comparable binding affinity toward the hydrophilic ink molecules.…”

Section: Resultsmentioning

confidence: 96%

“…Hydrophobic surfaces with hydrophilic patterns are useful in a variety of applications, including cell microarrays, drug screening, biomolecules detections, and microfluidics . There are mainly three approaches to create the hydrophilic–hydrophobic micropatterned surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…As each of the three approaches has its own advantages and disadvantages, simple and reliable methods of fabricating high resolution hydrophilic patterns on hydrophobic surfaces are still highly pursued. We recently developed a microfluidic method to fabricate polydopamine (PDA) arrays on the fluorinated ethylene propylene (FEP) surface based on a two‐layered microfluidic chip . PDA is an underwater adhesive polymer which adheres to a wide range of organic and inorganic materials, including fluorinated surfaces .…”

Section: Introductionmentioning

confidence: 99%

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Patterning Hydrophobic Surfaces by Negative Microcontact Printing and Its Applications

Zhou

et al. 2018

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Here, a negative microcontact printing method is developed to form hydrophilic polydopamine (PDA) patterns with micrometer resolution on hydrophobic including perfluorinated surfaces. In the process of the negative microcontact printing, a uniform PDA thin film is first formed on the hydrophobic surface. An activated polydimethylsiloxane (PDMS) stamp is then placed in contact with the PDA-coated hydrophobic surface. Taking advantage of the difference in the surface energy between the hydrophobic surface and the stamp, PDA is removed from the contact area after the stamp release. As a result, a PDA pattern complementary to the stamp is obtained on the hydrophobic surface. By using the negative microcontact printing, arrays of liquid droplets and single cells are reliably formed on perfluorinated surfaces. Microlens array with tunable focal length for imaging studies is further created based on the droplet array. The negative microcontact printing method is expected to be widely applicable in high-throughput chemical and biological screening and analysis.

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Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Patterning Hydrophobic Surfaces by Negative Microcontact Printing and Its Applications

Zhou

et al. 2018

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“…The adhesive properties of polydopamine has also proved useful in the formation of microarrays for high throughput biological analyses . Additionally, microspots of polydopamine deposited onto passivated surfaces have been used for protein analyses and to attach a single or a few viable bacteria to each spot, useful for multiparametric analysis of cell populations . Nanoporous graphene foams were coated with polydopamine for the absorption of membrane proteins and their subsequent proteolysis by trypsin, providing high‐throughput proteomic analysis of membrane proteins .…”

Section: Applications Of Catecholamine Polymersmentioning

confidence: 99%

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

301

223

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Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

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“…For example, a type of Teflon derivative, a fluorinated ethylene propylene (FEP) membrane, was recently developed as a fluorescence microarray substrate to provide an ultralow background signal in cytokine detection. Atop the FEP membrane, a polydopamine microspot array was fabricated for protein conjugation and this scheme yielded DLs for IL-1β, IL-6, and IL-10 of 8.91, 1.33, and 6.12 pg/mL, respectively [68]. …”

Section: Cytokine Sensors With Various Optical Modes Of Detectionmentioning

confidence: 99%

Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement

Singh

Truong

Reeves

et al. 2017

Sensors

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Protein biomarkers, especially cytokines, play a pivotal role in the diagnosis and treatment of a wide spectrum of diseases. Therefore, a critical need for advanced cytokine sensors has been rapidly growing and will continue to expand to promote clinical testing, new biomarker development, and disease studies. In particular, sensors employing transduction principles of various optical modalities have emerged as the most common means of detection. In typical cytokine assays which are based on the binding affinities between the analytes of cytokines and their specific antibodies, optical schemes represent the most widely used mechanisms, with some serving as the gold standard against which all existing and new sensors are benchmarked. With recent advancements in nanoscience and nanotechnology, many of the recently emerging technologies for cytokine detection exploit various forms of nanomaterials for improved sensing capabilities. Nanomaterials have been demonstrated to exhibit exceptional optical properties unique to their reduced dimensionality. Novel sensing approaches based on the newly identified properties of nanomaterials have shown drastically improved performances in both the qualitative and quantitative analyses of cytokines. This article brings together the fundamentals in the literature that are central to different optical modalities developed for cytokine detection. Recent advancements in the applications of novel technologies are also discussed in terms of those that enable highly sensitive and multiplexed cytokine quantification spanning a wide dynamic range. For each highlighted optical technique, its current detection capabilities as well as associated challenges are discussed. Lastly, an outlook for nanomaterial-based cytokine sensors is provided from the perspective of optimizing the technologies for sensitivity and multiplexity as well as promoting widespread adaptations of the emerging optical techniques by lowering high thresholds currently present in the new approaches.

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An ultralow background substrate for protein microarray technology

Cited by 16 publications

References 31 publications

Patterning Hydrophobic Surfaces by Negative Microcontact Printing and Its Applications

Patterning Hydrophobic Surfaces by Negative Microcontact Printing and Its Applications

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Emerging Cytokine Biosensors with Optical Detection Modalities and Nanomaterial-Enabled Signal Enhancement

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