An ultrasensitive and long-lasting chemiluminescence immunoassay for IP-10 detection based on a 4-bromophenol-reinforced bienzymatic system

Chang, Jiang; Zou, De-Ying; Ren, Hong-Lin; Li, Xilin; Li, Meng; Si, Zhaozhao; Chen, Han; Liu, Zengshan; Lu, Shiying; Pan, Hongge

doi:10.1016/j.microc.2022.107719

Cited by 2 publications

(2 citation statements)

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“…PAGE confirmed the viability of CHA amplification (Table S2). Tables S3 and S4 present the 13). Similarly, the feasibility of IP-10 CHA amplification was verified using a PAGE experiment (Figure 1k).…”

Section: ■ Experimental Sectionmentioning

confidence: 68%

“…Several immune-based approaches, including colorimetric, electrochemical, chemiluminescence (CL), electrochemiluminescence, fluorescence, and filter paper methods, have been reported to detect IP-10 and IFN-γ in recent years. − The enzyme-linked immunosorbent assay (ELISA) and CL assay are now the most commonly used techniques in clinical practice for measuring IP-10 and IFN-γ (Scheme B). These methods employ solid-phase carriers or magnetic beads to coat the antigens or antibodies.…”

Section: Introductionmentioning

confidence: 99%

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Nucleic Acid and Nanomaterial Synergistic Amplification Enables Dual Targets of Ultrasensitive Fluorescence Quantification to Improve the Efficacy of Clinical Tuberculosis Diagnosis

Shi,

Jiang,

Peng

et al. 2024

ACS Appl. Mater. Interfaces

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Interferon-γ (IFN-γ) release assays (IGRAs) are constrained by the limited diagnostic performance of a single indicator and the excessive Mycobacterium tuberculosis (Mtb) antigen stimulation time. This study presents a simultaneous, homogeneous, rapid, and ultrasensitive fluorescence quantification strategy for IFN-γ and IFN-γ-induced protein 10 (IP-10). This method relies on the high-affinity binding of aptamers to IFN-γ and IP-10, the enzyme-free catalytic hairpin assembly reaction, and the heightened sensitivity of CdTe quantum dots to Ag + and hairpin structure C-Ag + -C and carbon dots to Hg 2+ and hairpin structure T-Hg 2+ -T. Under optimized conditions, the selectivity of IFN-γ and IP-10 was excellent, with a linear range spanning from 1 to 100 ag/mL and low limits of detection of 0.3 and 0.5 ag/mL, respectively. Clinical practicality was confirmed through testing of 57 clinical samples. The dual-indicator combination detection showed 92.8% specificity and 93.1% sensitivity, with an area under the curve of 0.899, representing an improvement over the singleindicator approach. The Mtb antigen stimulation time was reduced to 8 h for 6/7 clinical samples. These findings underscore the potential of our approach to enhance the efficiency and performance of a tuberculosis (TB) clinical diagnosis.

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Section: ■ Experimental Sectionmentioning

confidence: 68%