An ultrasensitive and specific electrochemical biosensor for DNA detection based on T7 exonuclease-assisted regulatory strand displacement amplification

Li, Shengqiang; Fu, Zhengxiang; Wang, Chao; Shang, Xipeng; Zhao, Yan; Liu, Cuiying; Pei, Ming

doi:10.1016/j.aca.2021.338988

Cited by 7 publications

(3 citation statements)

References 36 publications

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“…To investigate the specificity of the proposed sensor for target sequences, the biosensor was applied to analyze porcine epidemic diarrheavirus (PEDV), porcine enteric alphacoronavirus (PEAV), double-base mismatched DNA (DM-DNA), and random sequence. 39 The results are shown in Fig. 6.…”

Section: Resultsmentioning

confidence: 97%

Electrochemical detection of SARS-CoV-2 based on copper nanoflower-triggeredin situgrowth of electroactive polymers

Zhou

et al. 2023

Analyst

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Section: Resultsmentioning

confidence: 97%

Electrochemical detection of SARS-CoV-2 based on copper nanoflower-triggeredin situgrowth of electroactive polymers

Zhou

et al. 2023

Analyst

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“…The analysis of nucleic acids with specific sequences is increasingly important for clinical diagnosis and disease treatment. For example, nucleic acids can be used for the rapid and efficient identification of infectious diseases, cancer, and many other diseases. − A wide variety of nucleic acids have been discovered for liquid biopsies, including microRNA, ctDNA, cfDNA, DNA methylation, etc. ,,− Despite of its great value of nucleic acid analysis, the analysis of nucleic acids in clinical samples remains extremely challenging due to the ultralow concentrations and low abundance. It means that the technologies must achieve a high sensitivity and specificity to distinguish specific sequences from closely relative molecules.…”

Section: The Detection Of Nucleic Acidsmentioning

confidence: 99%

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Zhang

Zhu

et al. 2023

Chem. Rev.

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Whole blood, as one of the most significant biological fluids, provides critical information for health management and disease monitoring. Over the past 10 years, advances in nanotechnology, microfluidics, and biomarker research have spurred the development of powerful miniaturized diagnostic systems for whole blood testing toward the goal of disease monitoring and treatment. Among the techniques employed for whole-blood diagnostics, electrochemical biosensors, as known to be rapid, sensitive, capable of miniaturization, reagentless and washing free, become a class of emerging technology to achieve the target detection specifically and directly in complex media, e.g., whole blood or even in the living body. Here we are aiming to provide a comprehensive review to summarize advances over the past decade in the development of electrochemical sensors for whole blood analysis. Further, we address the remaining challenges and opportunities to integrate electrochemical sensing platforms.

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“…For example, Liu et al investigated an electrochemical sensor with signal amplification achieved by signal output through hybridization chain reaction (HCR) to achieve better sensitivity, accuracy and stability [12]. Nucleic acid amplification strategies with electrochemical sensors have been successfully used for nucleic acid detection for nucleic acids [13], small molecules [14] and proteins [15].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical DNA Biosensors with Dual-Signal Amplification Strategy for Highly Sensitive HPV 16 Detection

Yang,

Liao,

Qing

et al. 2023

Sensors

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Cervical cancer is an important topic in the study of global health issues, ranking fourth among women’s cancer cases in the world. It is one of the nine major cancers that China is focusing on preventing and treating, and it is the only cancer that can be prevented through vaccination. Systematic and effective screening for human papilloma (HPV) infection, which is closely linked to the development of cervical cancer, can reduce cervical cancer incidence and mortality. In this paper, an electrochemical sensor was designed to detect HPV 16 using dual-signal amplification. An APTES-modified glassy carbon electrode was used for improved stability. Gold nanoparticles and a chain amplification reaction were combined for signal amplification. The limit of detection (LOD) of this electrochemical sensor was 1.731 × 10−16 mol/L, and the linear response of the target detector range was from 1.0 × 10−13 mol/L to 1.0 × 10−5 mol/L (R2 = 0.99232). The test of serum sample recovery showed that it has good anti-interference, and the performance of all aspects was improved to different degrees compared with the previous research from the team. The designed sensor is centered around the principles of low cost, high sensitivity and stability, which provides new ideas for the future development of cervical cancer prevention and electrochemical biosensors.

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An ultrasensitive and specific electrochemical biosensor for DNA detection based on T7 exonuclease-assisted regulatory strand displacement amplification

Cited by 7 publications

References 36 publications

Electrochemical detection of SARS-CoV-2 based on copper nanoflower-triggeredin situgrowth of electroactive polymers

Electrochemical detection of SARS-CoV-2 based on copper nanoflower-triggeredin situgrowth of electroactive polymers

Electrochemical Biosensors for Whole Blood Analysis: Recent Progress, Challenges, and Future Perspectives

Electrochemical DNA Biosensors with Dual-Signal Amplification Strategy for Highly Sensitive HPV 16 Detection

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