An ultrasensitive cathodic electrochemiluminescence immunoassay for thrombomodulin based on Ru(bpy)₃²⁺ encapsulated by MIL-NH₂-101(Al) nanocomposites

Abstract

A new type of cathodic electrochemiluminescence immunoassay based on Ru(bpy)32+ encapsulated MIL-NH2-101(Al) nanoparticles is designed for subtle exploration of the disseminated intravascular coagulation biomarker thrombomodulin firstly.

Sulfonic Acid-Functionalized Tetraphenylethylene-Amplified Electrochemiluminescence by Regulating π–π Interaction

“…Currently, numerous strategies have been developed to resolve the ACQ effect caused by the π–π accumulation of Ru­(bpy) 3 2+ in aqueous solution, thereby improving the ECL efficiency of such solution. For example, nanomaterials with large specific surface areas or cavity structures are introduced as carriers to support Ru­(bpy) 3 2+ , organic ligands with different coordination environments are introduced to provide interaction sites for the connection of Ru­(bpy) 3 2+ , and new luminous bodies, such as metal–organic frameworks (MOFs), are synthesized with Ru­(bpy) 3 2+ as ligands or guest molecules. − These strategies can regulate the distance between Ru­(bpy) 3 2+ molecules through steric effects or frame structures, thereby avoiding the reduction of the ECL intensity due to ACQ effects caused by the intermolecular aggregation of Ru­(bpy) 3 2+ . For instance, Liu’s group constructed an ideal ECL nanoreactor (Ru@zeolite) by assembling Ru­(bpy) 3 2+ molecules with nanoporous zeolites with a large specific surface area.…”