An ultrasensitive chemiluminescence immunosensor for PSA based on the enzyme encapsulated liposome

Zheng, Yue; Chen, Huan; Liu, Xueping; Jiang, Jian‐Hui; Luo, Yan; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.talanta.2008.07.038

Cited by 63 publications

(28 citation statements)

References 32 publications

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“…They are then added to a testing surface, typically a polystyrene microtiter plate, which has the antigen of interest immobilized on its surface. Zheng et al [11] have reported the use of horseradish peroxidase ( HRP ) encapsulating liposomes for the detection of prostate -specifi c antigen ( PSA ) (Figure 8.1 ).…”

Section: Detection Of Antigensmentioning

confidence: 99%

Lipid Nanoparticle‐Mediated Detection of Proteins

Nyren‐Erickson

Hendrickson

Mallik

2011

Biosensor Nanomaterials

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Section: Detection Of Antigensmentioning

confidence: 99%

Lipid Nanoparticle‐Mediated Detection of Proteins

Nyren‐Erickson

Hendrickson

Mallik

2011

Biosensor Nanomaterials

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“…Loaded liposomes were successfully applied in different immunassays, such as the microtiter-plate sorbent assay [8][9][10][11], flow-injection analysis [12][13][14], lateral flow on-site tests [15], chemiluminescent [16] and electrochemical [17] biosensors and also microarray [18].…”

Section: Introductionmentioning

confidence: 99%

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Beloglazova¹,

Goryacheva²,

Speranskaya³

et al. 2015

Talanta

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In: TALANTA, 134, 120-125, 2015. To refer to or to cite this work, please use the citation to the published version:Beloglazova N., Goryacheva O., Speranskaya E., Aubert T., Shmelin P. , Kurbangaleev V., Goryacheva I.Y., De Saeger S. (2015). Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay. TALANTA, 134,[120][121][122][123][124][125] AbstractThis manuscript describes synthesis and followed application of silica-coated liposomes loaded with quantum dots as a perspective label for immunoaasay. The hollow spherical structure of liposomes makes them an attractive package material for encapsulation of multiple water-insoluble quantum dots and amplifying the analytical signal. Silica coverage ensures the stability of the loaded liposomes against fusion and internal leakage during storage, transporting, application and also provides groups for bioconugation. For the first time these nanostructures were employed for the sensitive multiplex immunochemical determination of two analytes. As a model system mycotoxins zearalenone and aflatoxin B1 were detected in cereals. For simplification of multiassay results' evaluation the silanized liposomed loaded with QDs of different colors were used. The IC 50 values for the simultaneous determination of zearalenone and aflatoxin B1 were 16.2 and 18 µg kg -1 for zearalenone and 2.2 and 2.6 µg kg -1 for aflatoxin B1 in wheat and maize, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.

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“…The ELISA method is still being used, the most popular assays to quantify serum PSA concentration are immunometric assays or immunoradiometric assays with calibration ranges of 0-100 μg·L −1 [4,5]. Recently a variety of immunoassays have been developed for the detection of PSA, such as radioimmunoassay [6,7], chemiluminescence immunoassay [8,9], enzymelinked immunosorbent assay (ELISA) [10,11], fluoroimmunoassay [12,13], electrophoretic immunoassay [14], mass spectrometric immunoassay [15,16], and time-resolved immunofluorometric assay [17], etc. Despite many efforts that have been made in developing the sensors for PSA detection, certain challenges still need to be addressed such as hazardous to the health, or require highly personnel and sophisticated instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical immunosensor for prostate-specific antigen using a glassy carbon electrode modified with a nanocomposite containing gold nanoparticles supported with starch-functionalized multi-walled carbon nanotubes

et al. 2012

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We reported on a novel electrochemical immunosensor for the determination of prostate specific antigen (PSA). It is based on gold nanoparticles supported on crosslinked starch functionalized multi-walled carbon nanotubes in a nanocomposite film. The method is simple and can be carried out at room temperature without the use of expensive chemicals or corrosive acids. Thus, it preserves the integrity and the electronic structure of the nanotubes. The stepwise assembly of the electrode was characterized by cyclic voltammetry (CV) and impedance spectroscopy. CV studies demonstrated that the formation of antibody-antigen complex decreases the peak current of the hexacyanoferrate redox pair linearly with increasing PSA concentration in two ranges (0.01-0.5 and 0.5-3.0 ng·mL −1 ). The detection limit for PSA is 7 pg·mL −1 (at S/N03). This immunoassay exhibits good sensitivity and reproducibility and may become a promising technique for the diagnosis and monitoring of prostate cancer.

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An ultrasensitive chemiluminescence immunosensor for PSA based on the enzyme encapsulated liposome

Cited by 63 publications

References 32 publications

Lipid Nanoparticle‐Mediated Detection of Proteins

Lipid Nanoparticle‐Mediated Detection of Proteins

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Electrochemical immunosensor for prostate-specific antigen using a glassy carbon electrode modified with a nanocomposite containing gold nanoparticles supported with starch-functionalized multi-walled carbon nanotubes

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