An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry

An, Yu; Jin, Tongyu; Zhu, Yuyuan; Zhang, Fan; He, Pingang

doi:10.1016/j.bios.2019.111503

Cited by 141 publications

(75 citation statements)

References 34 publications

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“…[143] In addition, aptasensors have been developed for the detection of aptamers in cancer derived exosomes. [59,144] This kind of aptasensor is based on the detection of exosomes that use different types of sensitive detectors such as Surface plasmon resonance, electrochemical, etc. Another application of aptamer-targeted exosomes is as delivery systems of antineoplastics or other drugs.…”

Section: Theranostic Applications Of Exosomes In Cancermentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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Exosomes, a subgroup of extracellular vesicles, are important mediators of long‐distance intercellular communication and are involved in a diverse range of biological processes such as the transport of lipids, proteins, and nucleic acids. Researchers, seeing the problems caused by the toxic effects and clearance of synthetic nanoparticles, consider exosomes as an interesting alternative to such nanoparticles in the specific and controlled transport of drugs. In recent years, there have been remarkable advances in the use of exosomes in cancer therapeutics or for treating neurological diseases, among other applications. The objective of this work is to analyze studies focused on exosomes used in drug delivery system, present and future applications in this field of research are discussed based on the results obtained.

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Section: Theranostic Applications Of Exosomes In Cancermentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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show abstract

“…In this study, the intrinsic affinity between the aptamers and the membrane proteins of exosomes played a key role in exosomes capture. In addition, some scholars have established some detection methods of exosomes by combining surface proteins and aptamers recognition with other analysis methods, including colorimetric analysis, [64] surface plasma resonance (SPR), [65] giant magnetoresistance (GMR) biosensors, [66] click‐chemistry, [67] and luminescent resonant energy transfer (LRET) [68] . Tan's group [69] used the specific binding between exosomes surface proteins and aptamers to cause color changes.…”

Section: Methods Of Exosomes Detectionmentioning

confidence: 99%

“…The principle of detecting exosomes based on Ti3C2 MXenes biosensor ECL modified by in situ formation of gold nanoparticles. [59] magnetoresistance (GMR) biosensors, [66] click-chemistry, [67] and luminescent resonant energy transfer (LRET). [68] Tan's group [69] used the specific binding between exosomes surface proteins and aptamers to cause color changes.…”

Section: Protein Recognition Based On Aptamer Affinitymentioning

confidence: 99%

Recent Progress on the Isolation and Detection Methods of Exosomes

Jiang

Liu

2020

Chemistry An Asian Journal

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Exosomes are known as one of extracellular vesicles, which are found in various body fluids and released by cells. As transport carrier, exosomes participate actively in intercellular communication and reflect their characteristics uniquely to the origin cells. Due to their unique biological physical properties and physiological functions, exosomes are considered to be one of best biomarkers of cancer diagnosis. At the same time, exosomes are potential therapeutic targets and drug delivery carriers. Therefore, the characteristics, functions and analytical methods of exosomes have increasingly attracted wide attention among scientists. In this review, the recent research progress on the basic characteristics and functional applications of exosomes are summarized. Furthermore and importantly, this review focuses on the recent advance in the purification and test methods of exosomes in recent years. Finally, issues pertaining to exosome detection are presented. Based on newly discovered characteristic of exosomes, the opportunities and challenges for future research of the purification and quantitative detection methods are outlined.

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“…The aptasensor showed high selectivity and sensitivity of gastric cancer EVs and a LOD of 9.54 × 10 2 /mL. The electrochemical biosensor developed by An et al was based on click chemistry of alkynyl-4-ONE and the DNA hybridization chain reaction (HCR) to amplify the signal by using a streptavidin-biotin reaction for the determination of tumor EVs [83].…”

Section: Electrochemical-based Analysis Methods For Ev Detectionmentioning

confidence: 99%

Applications of Bionano Sensor for Extracellular Vesicles Analysis

2020

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Recently, extracellular vesicles (EVs) and their contents have been revealed to play crucial roles in the intrinsic intercellular communications and have received extensive attention as next-generation biomarkers for diagnosis of diseases such as cancers. However, due to the structural nature of the EVs, the precise isolation and characterization are extremely challenging. To this end, tremendous efforts have been made to develop bionano sensors for the precise and sensitive characterization of EVs from a complex biologic fluid. In this review, we will provide a detailed discussion of recently developed bionano sensors in which EVs analysis applications were achieved, typically in optical and electrochemical methods. We believe that the topics discussed in this review will be useful to provide a concise guideline in the development of bionano sensors for EVs monitoring in the future. The development of a novel strategy to monitor various bio/chemical materials from EVs will provide promising information to understand cellular activities in a more precise manner and accelerates research on both cancer and cell-based therapy.

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An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry

Cited by 141 publications

References 34 publications

Advances in Exosomes‐Based Drug Delivery Systems

Advances in Exosomes‐Based Drug Delivery Systems

Recent Progress on the Isolation and Detection Methods of Exosomes

Applications of Bionano Sensor for Extracellular Vesicles Analysis

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