An ultrasensitive enzyme-free electrochemical immunosensor for CA125 using Au@Pd core–shell nanoparticles as labels and platforms for signal amplification

Guo, Aiping; Wu, Dan; Ma, Hongmin; Zhang, Yihe; He, Limin; Du, Bin; Wei, Qin

doi:10.1039/c3tb20574f

Cited by 53 publications

(22 citation statements)

References 48 publications

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“…This immunoassay shows wide linear range (0.005-20.00 ng mL −1 ), low detection limit (1.3 pg mL −1 ), good reproducibility, selectivity and acceptable stability. Wei and his collaborator designed a facile and sensitive non-enzymatic sandwich-type immunoassay for the detection of carbohydrate antigen 125 (CA 125) based on Au@Pd core-shell nanoparticle as labels [83]. Au@Pd nanoparticles show high electrocatalytic activity toward hydrogen peroxide reduction and the encapsulated Au nanoparticles can also accelerate the electron transfer.…”

Section: Bimetallic Nanoparticlesmentioning

confidence: 99%

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Tang

2015

Microchim Acta

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Electrochemical immunodetection has attracted considerable attention due to its high sensitivity, low cost and simplicity. Large efforts have recently made in order to design ultrasensitive assays. Noble metal nanoparticles (NM-NPs) offer advantages such as high conductivity and large surface-to-volume ratio. NM-NPs therefore are excellent candidates for developing electrochemical platforms for immunodetection and as signal tags. The use of biofunctionalized NM-NPs often results in amplified recognition via stronger loading of signal tags, and also in enhanced signal. This review (with 87 references) gives an overview on the current state in the use of NM-NPs in Nonenzymatic electrochemical immunosensing. We discuss the application of NM-NPs as electrode matrices and as electroactive labels (either as a carrier or as electrocatalytic labels), and compare the materials (mainly nanoparticles of gold, platinum, or of bimetallic materials) in terms of performance (for example by increasing sensitivity via label amplification or via high densities of capture molecules). A conclusion covers current challenges and gives an outlook. Rather than being exhaustive, the review focuses on representative examples that illustrate novel concepts and promising applications. NM-NPs based immunosensing opens a series of concepts for basic research and offers new tools for determination of trace amounts of protein-related analytes in environment and clinical applications.

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Section: Bimetallic Nanoparticlesmentioning

confidence: 99%

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Tang

2015

Microchim Acta

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“…It was first detected over 30 years ago using the OC125 monoclonal antibody, which provides the basis for immunoassays to detect and monitor the progression of epithelial ovarian cancer. 6 Nevertheless, various innovative immunodetection technologies for the detection of CA125 have been reported, such as arrayed microsensor chips, 6 a bead-based enzyme-linked immunosorbent assay (ELISA), 7 a fluorescence immunoassay employing the ALYGNSA antibody-orientation system, 8 electrochemical biosensors, [9][10][11][12] and nanomaterial-based colorimetric immunoassays. 4,5 Conventional CA125 immunoassay has a detection limit of 15 U∕mL, which is sufficient to detect the threshold level of 35 U∕ml CA125 that correlates with the diseased state.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of alignment layer by ultraviolet irradiation to dramatically improve the detection limit of liquid-crystal-based immunoassay for the cancer biomarker CA125

Lee

2015

J. Biomed. Opt

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Liquid crystal (LC)-based biosensing has attracted much attention in recent years. We focus on improving the detection limit of LC-based immunoassay techniques by surface modification of the surfactant alignment layer consisting of dimethyloctadecyl[3-(trimethoxysilyl)propyl]ammonium chloride (DMOAP). The cancer biomarker CA125 was detected with an array of anti-CA125 antibodies immobilized on the ultraviolet (UV)-modified DMOAP monolayer. Compared with a pristine counterpart, UV irradiation enhanced the binding affinity of the CA125 antibody and reproducibility of immunodetection in which a detection limit of 0.01 ng∕ml for the cancer biomarker CA125 was achieved. Additionally, the optical texture observed under a crossed polarized microscope was correlated with the analyte concentration. In a proof-of-concept experiment using CA125-spiked human serum as the analyte, specific binding between the CA125 antigen and the anti-CA125 antibody resulted in a distinct and concentration-dependent optical response despite the high background caused by nonspecific binding of other biomolecules in the human serum. Results from this study indicate that UVmodification of the alignment layer, as well as detection with LCs of large birefringence, contributes to the enhanced performance of the label-free LC-based immunodetection, which may be considered a promising alternative to conventional label-based methods.

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“…2 Due to the importance of early diagnosis of cancer, various immunoassays for the detection of CA125 cancer biomarker have been reported, including enzyme-linked immunosorbent assay, 3 electrochemical immunoassay, [4][5][6][7] and colorimetric immunoassay. 8 These traditional label-based immunoassays are often complicated, timeconsuming, with the high cost of labeling antibodies.…”

mentioning

confidence: 99%

Label-free immunodetection of the cancer biomarker CA125 using high-Δn liquid crystals

Lee

et al. 2014

J. Biomed. Opt

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Abstract. A label-free and array-based optical liquid-crystal (LC) immunodetection technique for the detection of CA125 antigen, a protein biomarker most frequently used for ovarian cancer detection, was demonstrated with a nematic LC with larger birefringence (Δn) to promote sensitivity in detecting biomolecules. The LC-based immunodetection offers an alternative and sensitive approach for the detection of biomarker proteins, with the potential of replacing conventional immunoassays used in biochemical studies and clinical laboratories. © The Authors.Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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An ultrasensitive enzyme-free electrochemical immunosensor for CA125 using Au@Pd core–shell nanoparticles as labels and platforms for signal amplification

Cited by 53 publications

References 48 publications

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Non-enzymatic electrochemical immunoassay using noble metal nanoparticles: a review

Surface modification of alignment layer by ultraviolet irradiation to dramatically improve the detection limit of liquid-crystal-based immunoassay for the cancer biomarker CA125

Label-free immunodetection of the cancer biomarker CA125 using high-Δn liquid crystals

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