An ultrasensitive flow cytometric immunoassay based on bead surface-initiated template-free DNA extension

Zhu, Liping; Chen, Desheng; Lü, Xiaohui; Qi, Yan; He, Ping; Liu, Chenghui; Li, Zhengping

doi:10.1039/c8sc02752h

Cited by 36 publications

(26 citation statements)

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“…[ 50 ] LSRP results from strong localized electromagnetic fields that exhibited at metal nanostructures, which can realize sensitive detection of nanoscale biological analysts. [ 51 ] Thakur et al. developed a self‐assembly gold nanoislands (AuNIs) sensing chip combined with the LSPR biosensor to detect and differentiate exosomes from microvesicles (MVs) isolated from cells and real samples without antibody functionalization.…”

Section: Exosome Detection Methodologiesmentioning

confidence: 99%

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Xiong

Huang

Yang

et al. 2021

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101

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Exosomes, known as nanometer‐sized vesicles (30–200 nm), are secreted by many types of cells. Cancer‐derived exosomes have great potential to be biomarkers for early clinical diagnosis and evaluation of cancer therapeutic efficacy. Conventional detection methods are limited to low sensitivity and reproducibility. There are hundreds of papers published with different detection methods in recent years to address these challenges. Therefore, in this review, pioneering researches about various detection strategies are comprehensively summarized and the analytical performance of these tests is evaluated. Furthermore, the exosome molecular composition (protein and nucleic acid) profiling, a single exosome profiling, and their application in clinical cancer diagnosis are reviewed. Finally, the principles and applications of machine learning method in exosomes researches are presented.

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Section: Exosome Detection Methodologiesmentioning

confidence: 99%

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Xiong

Huang

Yang

et al. 2021

Small

101

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“…35 Isothermal amplication of nucleic acids (e.g., rolling circle amplication (RCA)) as an alternative amplication technique enables rapid and effective amplication at constant temperature. [35][36][37][38][39][40][41][42][43][44] RCA may generate very long single-stranded DNAs (ssDNAs) with tandem repeats, and it can achieve approximately 10 3 -fold amplication within 1 h. 35,[45][46][47] The visualization and analysis of RCA products usually use either SYBR green I as the uorescence label 45 or the uorescence-labeled oligonucleotide probe. 46 SYBR Green I is a DNA intercalating dye that binds dsDNA, 45 but it has several limitations including the concentration-dependent inhibition of PCR, preferential binding to the GC-rich sequences, the promotion of nonspecic amplication, and the detection of only a single type of target due to the use of a single uorophore, false positive signals due to its binding to any dsDNAs including nonspecic dsDNA sequences.…”

Section: Introductionmentioning

confidence: 99%

Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level

Chen

et al. 2020

Chem. Sci.

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“…Owing to the high stability, low cost, and convenient labeling, nanoparticles can also serve as signal probes for DNA detection to further improve the performance of sandwich assays [106][107][108][109][110][111][112][113][114][115]. For example, Zhang et al [116] reported a nanoparticle based "sandwich" detection strategy by immobilizing gold nanoparticles (AuNPs) with reporter probes that flank the target nucleic acids.…”

Section: +mentioning

confidence: 99%

Nucleic Acids Analysis

et al. 2020

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In recent years, multi-modal entity linking (MEL) has garnered increasing attention in the research community due to its significance in numerous multi-modal applications. Video, as a popular means of information transmission, has become prevalent in people's daily lives. However, most existing MEL methods primarily focus on linking textual and visual mentions or offline videos's mentions to entities in multi-modal knowledge bases, with limited efforts devoted to linking mentions within online video content. In this paper, we propose a task called Online Video Entity Linking (OVEL), aiming to establish connections between mentions in online videos and a knowledge base with high accuracy and timeliness. To facilitate the research works of OVEL, we specifically concentrate on live delivery scenarios and construct a live delivery entity linking dataset called LIVE. Besides, we propose an evaluation metric that considers timelessness, robustness, and accuracy. Furthermore, to effectively handle OVEL task, we leverage a memory block managed by a Large Language Model and retrieve entity candidates from the knowledge base to augment LLM performance on memory management. The experimental results prove the effectiveness and efficiency of our method.

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An ultrasensitive flow cytometric immunoassay based on bead surface-initiated template-free DNA extension

Abstract: An ultrasensitive flow cytometric immunoassay (TdT-FCI) is developed based on bead surface-initiated template-free DNA extension.

Cited by 36 publications

References 50 publications

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Rolling circle amplification-driven encoding of different fluorescent molecules for simultaneous detection of multiple DNA repair enzymes at the single-molecule level

Nucleic Acids Analysis

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