An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

Wang, Zecheng; Zhang, Chenchen; He, Shiwen; Xu, Danke

doi:10.1016/j.talanta.2022.124221

Cited by 12 publications

(5 citation statements)

References 34 publications

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“…The antigen-based half-life assay is a test based on the destruction of the viral surface, which is different from cell culture and viral nucleic acid assays; therefore, direct detection of SARS-CoV-2 antigen may more appropriately reflect infectivity compared to RNA detection (Wang et al 2003a ; Peck Palmer et al 2023 ; Hillig et al 2023 ; Wang et al 2023b ); our results could be more reliable. Moreover, the half-life of the RNA determined by PCR could be more than the actual duration of infectivity.…”

Section: Discussionmentioning

confidence: 86%

Determining half-life of SARS-CoV-2 antigen in respiratory secretion

Guang

Liu

2023

Environ Sci Pollut Res

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is primarily transmitted from person to person through respiratory droplets and aerosols. It is also possible for the virus to be transmitted indirectly through environmental contamination. The likelihood of environmental transmission depends on several factors, including the survival time of the virus in respiratory secretions. However, the stability of SARS-CoV-2 in respiratory secretions has not been investigated. In this study, we compared the half-life of the SARS-CoV-2 antigen in respiratory secretion under different conditions. We applied respiratory secretion (5 µL) to glass slides, air-dried the slides for 1 h, and kept them at 24 °C or 4 °C for 10 days. Respiratory secretions were also placed in test tubes (sealed to preserve moisture) and in normal saline for 10 days. The concentration of SARS-CoV-2 antigen in all samples was simultaneously measured using colloidal gold immunochromatography, and the half-life of the antigen was calculated. The half-life of the antigen in the wet (sealed tube) and saline samples at room temperature was 5.0 and 2.92 days, respectively. The half-life of the antigen in the air-dried sample at room temperature and at 4 °C was 2.93 and 11.4 days, respectively. The half-life was longer in respiratory secretions than that in normal saline. The half-life was also longer in respiratory secretions, at a lower temperature, and under wet conditions. Therefore, environmental transmission can also play a significant role in the spread of the virus. Robust prevention and control strategies could be developed based on the half-life of the antigen in respiratory secretions.

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Section: Discussionmentioning

confidence: 86%

Determining half-life of SARS-CoV-2 antigen in respiratory secretion

Guang

Liu

2023

Environ Sci Pollut Res

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“…In contrast, the addition of a target protein induces the formation of a protein-aptamer complex, resulting in the aptamer not acting as a primer, inhibiting the RCA reaction and fluorescence signal generation. As another example, Wang et al reported a sandwichtype aptasensor that amplifies the fluorescence signal through an RCA reaction to detect the SARS-CoV-2 antigen [102]. This aptasensor first captures a target using an antibody immobilized on a well plate and then forms an antibody-target-aptamer sandwich complex by the added aptamer.…”

Section: Enzyme-assisted Signal Amplification Strategiesmentioning

confidence: 99%

Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors

Lee,

Shin,

Kim

et al. 2023

Molecules

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Aptamers have been spotlighted as promising bio-recognition elements because they can be tailored to specific target molecules, bind to targets with a high affinity and specificity, and are easy to chemically synthesize and introduce functional groups to. In particular, fluorescent aptasensors are widely used in biological applications to diagnose diseases as well as prevent diseases by detecting cancer cells, viruses, and various biomarkers including nucleic acids and proteins as well as biotoxins and bacteria from food because they have the advantages of a high sensitivity, selectivity, rapidity, a simple detection process, and a low price. We introduce screening methods for isolating aptamers with q high specificity and summarize the sequences and affinities of the aptamers in a table. This review focuses on aptamer-based fluorescence detection sensors for biological applications, from fluorescent probes to mechanisms of action and signal amplification strategies.

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“…Reproduced with permission from Ref. [ 103 ]. D Schematic representation of proposed platform for detection of COVID-19.…”

Section: Introductionmentioning

confidence: 99%

“…Wang et al developed a fluorescent aptasensor based on hyperbranched rolling cycle amplification (HRCA) for the detection of SARS-CoV-2 S1 protein and pseudovirus (Fig. 5 C) [ 103 ]. When preparing circular templates (CT), padlock and complementary DNA (CDNA) were incubated in T4 DNA ligase reaction buffer.…”

Section: Introductionmentioning

confidence: 99%

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

Tekin,

Kul,

Sagdic

et al. 2024

Microchim Acta

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The COVID-19 pandemic underlines the need for effective strategies for controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents the potential of nanomaterial-enabled optical biosensors for rapid and low-cost detection of SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, and surface plasmon resonance detection methods. Nanomaterials including metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods, nanoporous materials, nanoshell materials, and magnetic nanoparticles employed in the production of optical biosensors are presented in detail. This review also discusses the detection principles, fabrication methods, nanomaterial synthesis, and their applications for the detection of SARS-CoV-2 in four categories: antibody-based, antigen-based, nucleic acid–based, and aptamer-based biosensors. This critical review includes reports published in the literature between the years 2021 and 2024. In addition, the review offers critical insights into optical nanobiosensors for the diagnosis of COVID-19. The integration of artificial intelligence and machine learning technologies with optical nanomaterial-enabled biosensors is proposed to improve the efficiency of optical diagnostic systems for future pandemic scenarios. Graphical Abstract

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An ultrasensitive fluorescence aptasensor for SARS-CoV-2 antigen based on hyperbranched rolling circle amplification

Cited by 12 publications

References 34 publications

Determining half-life of SARS-CoV-2 antigen in respiratory secretion

Determining half-life of SARS-CoV-2 antigen in respiratory secretion

Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

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