An ultrasensitive impedimetric glycan biosensor with controlled glycan density for detection of lectins and influenza hemagglutinins

Hushegyi, András; Bertók, Tomáš; Damborský, Pavel; Katrlı́k, Jaroslav; Tkac, Jan

doi:10.1039/c5cc00922g

Cited by 57 publications

(50 citation statements)

References 71 publications

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“…Mixed SAMs with control over the density of sialic acid residues were developed by Hushegyi et al . (see above) to form impedimetric glycan biosensors for the detection of the influenza A virus (IAV) hemagglutinins (HAs) in the attomolar range . Aptamer‐based sensors (aptasensors) were developed by Bai and coworkers for the detections of IAV .…”

Section: Multivalent Studies At Functionalized Platformsmentioning

confidence: 99%

“…This method has been used for a large variety of applications such as the formation of micro-arrays and (bio) sensors for multivalent interactions. [30] Impedimetric glycan biosensors have been developed using mixtures of thiols on gold substrates by Hushegyi et al [31] Mixed SAMs of sialic acid residues were made with controlled ligand densities. Specifically, mixtures of 11-mercaptoundecanoic acid (MUA) with 6-mercaptoexanol were used for the functionalization of gold substrates, with MUA molar fractions ranging from 5 % to 50 %.…”

Section: Self-assembled Monolayers (Sams)mentioning

confidence: 99%

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Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems

Iorio

Huskens

2020

ChemistryOpen

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In the study of multivalent interactions at interfaces, as occur for example at cell membranes, the density of the ligands or receptors displayed at the interface plays a pivotal role, affecting both the overall binding affinities and the valencies involved in the interactions. In order to control the ligand density at the interface, several approaches have been developed, and they concern the functionalization of a wide range of materials. Here, different methods employed in the modification of surfaces with controlled densities of ligands are being reviewed. Examples of such methods encompass the formation of self‐assembled monolayers (SAMs), supported lipid bilayers (SLBs) and polymeric layers on surfaces. Particular emphasis is given to the methods employed in the study of different types of multivalent biological interactions occurring at the functionalized surfaces and their working principles.

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Section: Multivalent Studies At Functionalized Platformsmentioning

confidence: 99%

Section: Self-assembled Monolayers (Sams)mentioning

confidence: 99%

Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems

Iorio

Huskens

2020

ChemistryOpen

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“…It was demonstrated using RCA-based impedimetric sensors that changes in the glycan structure of antibodies isolated from human serum are closely associated with rheumatoid arthritis [70]. It is possible to use SAM-coated Au electrodes modified with an oligosaccharide to detect lectin and influenza hemagglutinin [71,72]. Bueno and coworkers have prepared lectin-immobilized sensors for studying lectin-glycoprotein interactions based on the impedimetric and capacitive responses of the sensors [73,74].…”

Section: Lectin-based Sensors For Glycoproteinsmentioning

confidence: 99%

Recent Progress in Electrochemical Biosensors for Glycoproteins

Akiba

Anzai

2016

Sensors

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This review provides an overview of recent progress in the development of electrochemical biosensors for glycoproteins. Electrochemical glycoprotein sensors are constructed by combining metal and carbon electrodes with glycoprotein-selective binding elements including antibodies, lectin, phenylboronic acid and molecularly imprinted polymers. A recent trend in the preparation of glycoprotein sensors is the successful use of nanomaterials such as graphene, carbon nanotube, and metal nanoparticles. These nanomaterials are extremely useful for improving the sensitivity of glycoprotein sensors. This review focuses mainly on the protocols for the preparation of glycoprotein sensors and the materials used. Recent improvements in glycoprotein sensors are discussed by grouping the sensors into several categories based on the materials used as recognition elements.

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“…The limit of detection of this biosensor was found to be 234 cells/mL. The Tkac group used a glycan-based EIS biosensor for the detection of different types of lectins and influenza viruses H1N1, H5N1 and H3N2 [28, 29]. The group was successful in detecting attomolar concentrations of lectins and influenza using their glycan-based EIS biosensor.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

Bhattarai

Tan

Pandey

et al. 2016

Journal of Electroanalytical Chemistry

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The interactions of the lectin Concanavalin A (Con A) with self-assembled monolayers (SAMs) of thiolated mono-, di-, and tri-mannosides were studied on the surface of gold wires using electrochemical impedance spectroscopy (EIS). The SAMs of mannosides were prepared either pure or along with thiolated triethylene glycol (TEG) at different molar ratios (1:1, 1:2, 1:4, 1:9, and 1:19) to better understand and optimize the interaction conditions. The charge-transfer resistance of the [Fe(CN)6]3−/4− redox probe was compared before and after the interaction at different concentrations of Con A to determine the equilibrium dissociation constant (Kd) and limit of detection (LOD). Values of Kd were found in the nanomolar range showing multivalent interactions between mannosides and Con A, and LOD was found ranging from 4–13 nM depending on the type of mannoside SAM used. Analysis using the Hill equation suggests negative cooperativity in the binding behavior. Peanut agglutinin was used as a negative control, and cyclic voltammetry was used to further support the experiments. We have found that neither the pure nor the widely dispersed monolayers of mannosides provide the conditions for optimal binding of Con A. The binding of Con A to these SAMs is sensitive to the molar ratio of the mannoside used to prepare the SAM and to the structure of the mannoside. A simple cleaning method has also been shown to regenerate the used gold wire electrodes. The results from these experiments contribute to the development of simple, cheap, selective, and sensitive EIS-based bioassays, especially for lectin–carbohydrate interactions.

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An ultrasensitive impedimetric glycan biosensor with controlled glycan density for detection of lectins and influenza hemagglutinins

Abstract: An impedimetric glycan biosensor with optimised glycan density was applied for the detection of lectins and influenza hemagglutinins down to attomolar concentrations (aM).

Cited by 57 publications

References 71 publications

Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems

Surface Modification with Control over Ligand Density for the Study of Multivalent Biological Systems

Recent Progress in Electrochemical Biosensors for Glycoproteins

Electrochemical impedance spectroscopy study of Concanavalin A binding to self-assembled monolayers of mannosides on gold wire electrodes

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