An ultrasensitive nanofiber-based assay for enzymatic hydrolysis and deep-sea microbial degradation of cellulose

Tsudome, Mikiko; Tachioka, Mikako; Miyazaki, Masaru; Uchimura, Kohsuke; Tsuda, Miwako; Takaki, Yoshihiro; Deguchi, Shigeru

doi:10.1016/j.isci.2022.104732

Cited by 7 publications

(22 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protocol below describes a method for measuring the activity of cellulases on nanofibrous cellulose. 1 The quantitative assay of enzymatic hydrolysis of cellulose has usually been insensitive and time-consuming, primarily owing to the recalcitrance and water-insolubility of crystalline cellulose. This newly developed protocol is the first to successfully quantify the nanoscale enzymatic hydrolysis of cellulose.…”

Section: Before You Beginmentioning

confidence: 99%

“…This protocol can also be used to assay the microbial hydrolysis of nanofibrous cellulose. 1 Note: This protocol was optimized for commercially available cellulase (Meicelase, Meiji Seika) at a concentration of 10 mg/mL and a nanofibrous cellulose gel containing 3% cellulose (Kyokuto Pharmaceutical). …”

Section: Before You Beginmentioning

confidence: 99%

“…Endoglucanase alone cannot hydrolyze nanofibrous cellulose. 1 A cellulase capable of degrading crystalline cellulose can be used for quantitative analysis. This protocol uses Meicelase, a mixture of fungal cellulases from Trichoderma viride.…”

Section: Materials and Equipmentmentioning

confidence: 99%

“…The measured mass of hydrolyzed cellulose increased linearly with the reaction time up to 15 min, and the growth slowed thereafter. 1 The initial hydrolysis rates, which are obtained from the slope of linear fits to the data points within 15 min, are between 60 and 180 pg min -1 and proportional to the amount of deposited cellulases. 1 …”

Section: Expected Outcomesmentioning

confidence: 99%

“… 1 The initial hydrolysis rates, which are obtained from the slope of linear fits to the data points within 15 min, are between 60 and 180 pg min -1 and proportional to the amount of deposited cellulases. 1 …”

Section: Expected Outcomesmentioning

confidence: 99%

See 4 more Smart Citations

Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology

2023

Self Cite

View full text Add to dashboard Cite

Section: Before You Beginmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

Section: Materials and Equipmentmentioning

confidence: 99%

Section: Expected Outcomesmentioning

confidence: 99%

Section: Expected Outcomesmentioning

confidence: 99%

See 3 more Smart Citations

Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology

2023

Self Cite

View full text Add to dashboard Cite

Green alkaline fractionation of sugarcane bagasse at cold temperature improves digestibility and delignification without the washing processes and release of hazardous waste

Zahoor

Madadi

Nazar

et al. 2023

Industrial Crops and Products

View full text Add to dashboard Cite

Deep-Sea-Inspired Chemistry: A Hitchhiker’s Guide to the Bottom of the Ocean for Chemists

Deguchi,

Degaki,

Taniguchi

et al. 2023

Langmuir

Self Cite

View full text Add to dashboard Cite

The ocean constitutes approximately 70% of Earth's surface. Its average depth is 3688 m, of which depths beyond 200 m are classified as the deep sea. The deep sea is distinct from the surface of the ocean in terms of pressure, temperature, and sunlight. The unique physicochemical processes under the extreme environment of the deep sea and the specialized biochemical mechanisms developed by organisms to survive in the deep sea can serve as a vast source of inspiration for scientific and technological advancements. In this Perspective, we discuss three examples of deep-sea-inspired chemistry: (1) soft materials that respond to high pressures such as those observed in the deep sea; (2) molecular self-assembly inspired by the chemistry of hot and compressed water in deep-sea hydrothermal vents; and (3) nanobiotechnology and biomimetics inspired by survival strategies of deep-sea organisms. Finally, we provide an outlook on deep-sea-inspired chemistry. This Perspective aims to promote the sustainable utilization of the ocean based on knowledge, as opposed to the conventional utilization of the ocean solely based on resources. We hope that this Perspective will encourage chemists to harness their inspiration gleaned from the deep sea.

show abstract

An ultrasensitive nanofiber-based assay for enzymatic hydrolysis and deep-sea microbial degradation of cellulose

Cited by 7 publications

References 80 publications

Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology

Protocol for analyzing enzymatic hydrolysis of cellulose using surface pitting observation technology

Green alkaline fractionation of sugarcane bagasse at cold temperature improves digestibility and delignification without the washing processes and release of hazardous waste

Deep-Sea-Inspired Chemistry: A Hitchhiker’s Guide to the Bottom of the Ocean for Chemists

Contact Info

Product

Resources

About