An ultrasensitive sandwich chemiluminescent enzyme immunoassay based on phage-mediated double-nanobody for detection of Salmonella Typhimurium in food

Advances, applications, and limitations of portable and rapid detection technologies for routinely encountered foodborne pathogens

“…However, with the inclusion of an efficient enrichment protocol, the approach was able to distinguish proliferative or viable cells apart from dead cells. Similarly, a double nanobody sandwich chemiluminescent enzyme immunoassay with bacteriophage mediation reported a LOD of 3.63 × 10 3 CFU/ml (S. Typhimurium) in multiple food samples after 6-8 h incubation of fewer than 10 bacterial target cells (Zhang et al, 2022). The chemiluminescence method in the assay attempted to replace the conventional chromogenic reaction, and the bacteriophage mediation further enhanced the sensitivity.…”

Specific detection of viable Cronobacter sakazakii in powdered infant formula by phage amplification combined with qPCR (PAA‐qPCR) assay

“…Bacteriophages (phages) are viruses that specifically lyse host bacteria and have been extensively used since they are cheaper and are easily acquired biorecognition elements for rapid assay foodborne pathogens (Wang et al 2021a;Zhang et al 2022). Bacteriophages can distinguish live from dead bacteria since phages only multiply within living host cells (Huang et al 2021).…”

“…2021a; 2021b; Zhang et al . 2022). Bacteriophages can distinguish live from dead bacteria since phages only multiply within living host cells (Huang et al .…”

Two-stage nicking enzyme signal amplification (NESA)-based biosensing platform for the ultrasensitive electrochemical detection of pathogenic bacteria

“…Unfortunately, antibody instability under room temperature limits the applications of these methods. 10–12…”

“…Unfortunately, antibody instability under room temperature limits the applications of these methods. [10][11][12] Aptamers are single-stranded DNA or RNA oligonucleotides selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX) on the basis of their ability to bind target biomolecules with high affinity and specicity. [13][14][15][16] There are several advantages to the use of aptamers as compared to antibodies: aptamers bind target molecules with affinity and specicity equal or superior to those of antibodies, they are easily synthesized and modied, and they are more stable than antibodies.…”