An Ultrasensitive Upconversion Fluorescence Aptasensor Based on Graphene Oxide Release and Magnetic Separation for Staphylococcus aureus Detection

Chen, Min; Song, Yaqi; Han, Lu; Zhou, Dandan; Pan, Leiqing; Tu, Kang

doi:10.1007/s12161-022-02336-w

Cited by 16 publications

(7 citation statements)

References 32 publications

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“…The limit of detection (LOD) of the fabricated upconversion fluorescence probe was determined to be 30 CFU/mL, based on the formula of LOD = 3 Sa/S b (3 is the factor at the 99% confidence level, Sa is the standard deviation of 10 blank upconversion fluorescence measurements, and S b is the slope of the Shigella detection calibration plot). Furthermore, in comparison with the linear range, LOD and detection time of the developed upconversion fluorescence probe with other reported methods [ 33 , 34 , 35 , 36 , 37 , 38 ] confirmed that the probe can achieve Shigella detection with high sensitivity and meets the requirements for contaminated sample detection ( Table 1 ).…”

Section: Resultssupporting

confidence: 60%

“…As shown in Figure 2 A, five stretching vibration bands at 1469 cm −1 , 1572 cm −1 , 2849 cm −1 , 2934 cm −1 , and 3401 cm −1 were found, and these bands belonged to the =C-H, COO- and -CH-stretching vibration, respectively (curve a) [ 32 ]. After surface modification with ADA, the four bands at around 1469 cm −1 , 1572 cm −1 , 2849cm −1 , and 2934 cm −1 disappeared, and a new stretching vibration band at 1093 cm −1 appeared, which was associated with the NH 2 -stretching vibration, validating successful modification of ADA (curve b) [ 33 ]. After surface modification with carboxyl, the band at around 3467 cm −1 disappeared, and two new stretching vibration bands at 1502 cm −1 and 1701cm −1 appeared.…”

Section: Resultsmentioning

confidence: 94%

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Upconversion Fluorescence Nanoprobe-Based FRET for the Sensitive Determination of Shigella

et al. 2022

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Shigella as a typical foodborne pathogen has strong survivability in the environment or food, leading to infectious diseases, yet its rapid detection technology with high selectivity and sensitivity remains challenging. In this study, complementary strand modified upconversion nanoparticles (UCNPs) can offer stable yellow-green fluorescence at 500–700 nm excited by a 980 nm laser. Importantly, Shigella aptamer modified gold nanoparticles (GNPs) formed by “Au−S” bond act as a fluorescence resonance energy transfer (FRET) donor and recognition element that can bind specifically to Shigella and significantly quench the fluorescence of complementary strand modified UCNPs. As a result, the fluorescence of our developed nanoprobe increased linearly with the increase in Shigella in a wide range from 1.2 × 102 to 1.2 × 108 CFU/mL and the detection limit was as low as 30 CFU/mL. Moreover, the fabricated upconversion fluorescence nanoprobe can achieve Shigella detection in contaminated chicken without enrichment in 1 h.

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 94%

Upconversion Fluorescence Nanoprobe-Based FRET for the Sensitive Determination of Shigella

et al. 2022

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“…Recently, Chen et al (2022b) developed a fluorescence biosensor based on magnetic Fe 3 O 4 -modified graphene oxide (MNPs@GO) for rapid and direct diagnosis of S. aureus . The biosensor employed aptamer-functionalized upconversion nanoparticles (UCNPs) in combination with MNPs@GO.…”

Section: Application Of Mnps-based Biosensor For Poctmentioning

confidence: 99%

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Wang,

Jin,

Hang-Mei Leung

et al. 2024

Front. Bioeng. Biotechnol.

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The significance of point-of-care testing (POCT) in early clinical diagnosis and personalized patient care is increasingly recognized as a crucial tool in reducing disease outbreaks and improving patient survival rates. Within the realm of POCT, biosensors utilizing magnetic nanoparticles (MNPs) have emerged as a subject of substantial interest. This review aims to provide a comprehensive evaluation of the current landscape of POCT, emphasizing its growing significance within clinical practice. Subsequently, the current status of the combination of MNPs in the Biological detection has been presented. Furthermore, it delves into the specific domain of MNP-based biosensors, assessing their potential impact on POCT. By combining existing research and spotlighting pivotal discoveries, this review enhances our comprehension of the advancements and promising prospects offered by MNP-based biosensors in the context of POCT. It seeks to facilitate informed decision-making among healthcare professionals and researchers while also promoting further exploration in this promising field of study.

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“…The MNPs@GO-UCNP biosensor is able to detect quantitatively 13 CFU/mL. This biosensor was tested on real chicken meat samples, proving a valid system to monitor bacteria in food samples [ 82 ].…”

Section: Fluorescence-based Go Sensors For Diagnosticsmentioning

confidence: 99%

Biosensing Systems Based on Graphene Oxide Fluorescence Quenching Effect

Battisti

Samal²,

Puppi

2023

Micromachines

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Graphene oxide (GO) is a versatile material obtained by the strong oxidation of graphite. Among its peculiar properties, there is the outstanding ability to significantly alter the fluorescence of many common fluorophores and dyes. This property has been exploited in the design of novel switch-ON and switch-OFF fluorescence biosensing platforms for the detection of a plethora of biomolecules, especially pathological biomarkers and environmental contaminants. Currently, novel advanced strategies are being developed for therapeutic, diagnostic and theranostic approaches to widespread pathologies caused by viral or bacterial agents, as well as to cancer. This work illustrates an overview of the most recent applications of GO-based sensing systems relying on its fluorescence quenching effect.

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An Ultrasensitive Upconversion Fluorescence Aptasensor Based on Graphene Oxide Release and Magnetic Separation for Staphylococcus aureus Detection

Cited by 16 publications

References 32 publications

Upconversion Fluorescence Nanoprobe-Based FRET for the Sensitive Determination of Shigella

Upconversion Fluorescence Nanoprobe-Based FRET for the Sensitive Determination of Shigella

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Biosensing Systems Based on Graphene Oxide Fluorescence Quenching Effect

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