An ultrastructural and immunohistological study of the rat olfactory epithelium: Unique properties of olfactory sensory cells

Vollrath, M.; Altmannsberger, M.; Weber, Klaus; Osborn, Mary

doi:10.1111/j.1432-0436.1985.tb00323.x

Cited by 80 publications

(39 citation statements)

References 48 publications

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“…They concluded that the olfactory epithelium retains an important juvenile biological function and that its neurons express a protein characteristic of neuronal precursors and young neurons. Considering the disagreement between the authors who studied the expression of IFP in the olfactory neurons (Vollrath et al, 1985;Schwab et al, 1986;Talamo et al, 1989), it will be important to reconsider the problem. If peripherin is really the only IFP to be expressed there, it would be the only case where it is expressed alone, since we know that it is coexpressed with vimentin in mouse neuroblastoma cell lines (Portier et al, 1984a), with NFP in PC12 (Franke et al, 1986) and in the neuronal cell populations where it is expressed (Djabali, unpublished observations).…”

Section: Discussionmentioning

confidence: 99%

“…As to the expression of IFP, Vollrath et al (1985) observed that none of the antibodies to IFP decorated the sensory neurons of the olfactory epithelium and proposed that the olfactory sensory neurons are not postmitotic as other neurons are, but constantly renew (Graziadei and Monti-Graziadei, 1979a, b). However, Schwab et al (1986) demonstrated that the majority of olfactory neurons and their axons contain vimentin or a protein of similar immunological character and electrophoretic mobility.…”

Section: Discussionmentioning

confidence: 99%

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Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

et al. 1990

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The expression of peripherin, an intermediate filament protein, had been shown by biochemical methods to be localized in the neurons of the PNS. Using immunohistochemical methods, we analyzed this expression more extensively during the development of the rat and compared it with that of the low-molecular-mass neurofilament protein (NF-L), which is expressed in every neuron of the CNS and PNS. The immunoreactivity of NF-L is first apparent at the 25-somite stage (about 11 d) in the ventral horn of the spinal medulla and in the posterior part of the rhombencephalon. The immunoreactivity of peripherin appears subsequently, first colocalized with that of NF-L. Both immunoreactivities then spread out along rostral and caudal directions, but whereas the immunoreactivity of NF-L finally becomes noticeable in every part of the nervous system, that of peripherin remains localized to (1) the motoneurons of the ventral horn of the spinal medulla; (2) the autonomic ganglionic and preganglionic neurons; and (3) the sensory neurons. These results demonstrate that, in the neurons that originate from migrating neural crest cells, the immunoreactivities of peripherin and of NF-L become apparent only when they have reached their destination. The results also show that peripherin is expressed more widely than has been previously observed and that this protein occurs in neuronal populations from different lineages (neural tube, neural crest, placodes) with different functions (motoneurons, sensory and autonomic neurons). The common point of these neurons is that they all have axons lying, at least partly, at the outside of the axis constituted by the encephalon and the spinal medulla; this suggests that peripherin might play a role in the recognition of the axonal pathway through the intermediary of membrane proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential expression of two neuronal intermediate-filament proteins, peripherin and the low-molecular-mass neurofilament protein (NF-L), during the development of the rat

et al. 1990

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“…Localization of Pax-6 to sustentacular cells, HBCs, Bowman's glands, and a few cells in the middle of the epithelium may reflect a relationship between the four cell types and is consistent with a progenitor role for HBCs and/or Bowman's glands in the establishment of the sustentacular cell population (Mulvaney and Heist, 1971;Hempstead and Morgan, 1983;Suzuki and Takeda, 1991). Several other markers colocalize in these cell types, including SUS-1 and a collection of cytokeratins (Hempstead and Morgan, 1983;Vollrath et al, 1985;Levey et al, 1991). Indirect evidence from retroviral lineage analysis in adult OE, however, suggests a sustentacular lineage distinct from HBCs (Caggiano et al, 1994).…”

Section: Pax-6 Protein In Oe and Retinamentioning

confidence: 99%

“…Two classes of basal cells can be distinguished in the OE on the basis of their location, morphology, immunohistochemical reactivity, and distinct roles in OE development (Cuschieri and Bannister, 1975a,b;Vollrath et al, 1985;Calof and Chikaraishi, 1989;Levey et al, 1991;Suzuki and Takeda, 1991;Caggiano et al, 1994). Pax-6-positive cells located in the bottom-most layer of the epithelium are NCAM-negative, suggesting that HBCs, but not GBCs, express Pax-6.…”

Section: Pax-6 Protein In Oe and Retinamentioning

confidence: 99%

Role of Olf-1 and Pax-6 Transcription Factors in Neurodevelopment

1996

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The Olf-1 transcription factor is expressed in olfactory sensory neurons where it regulates the expression of genes that encode components of the odorant signal transduction cascade and contributes to the terminal phenotype of these sensory neurons. We examined the pattern of expression of Olf-1 protein during mouse embryogenesis and observed Olf-1 expression transiently in a subset of neural precursor cells in the CNS and peripheral nervous system. The expression of Olf-1 protein was enriched in sensory components and coincided with postmitotic cells and the initiation of overt differentiation within the nervous system. The spatial and temporal patterns of Olf-1 expression during development suggest a role in neurogenesis that is common among different neural cell types. In parallel, the expression pattern of Pax-6, a transcription factor that is widely expressed in the developing nervous system, including the visual and olfactory systems, was examined with a C-terminal antibody. In the retina, Pax-6 protein is detected in the lens, the cornea, and the neural and pigmented retinas. In the olfactory epithelium, Pax-6 protein is expressed exclusively in cells of non-neuronal lineage, including sustentacular cells, basal cells, and Bowman's glands. The nonoverlapping, cellular localization patterns of Pax-6 and Olf-1 demarcate distinct cell lineages within the developing olfactory epithelium.

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“…The olfactory epithelium of the rat consists of supporting cells and sensory cells as well as light and dark basal cells (12). Among these cell types, the light basal cells have undifferentiated, primitive &dquo;neuroblastic&dquo; nature, showing a large nucleus containing finely dispersed chromatin, abundant polyribosomes, a few special cell organelles, and no positive immunoreactions for keratin, vimentin, neurofilaments, GFAP, and desmin (12). The present tumor cells disclosed a close morphological and immunohistochemical similarity to the olfactory light basal cells, except that the former had welldeveloped RER and Golgi apparatus.…”

Section: Discussionmentioning

confidence: 99%