An ultrastructural classification of the neuronal cell bodies of the rat dorsal root ganglion using zinc iodide-osmium impregnation

Duce, I.R.; Keen, P.

doi:10.1007/bf00220670

Cited by 128 publications

(39 citation statements)

References 14 publications

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“…As criteria for dorsal root ganglion cell identification, dorsal root ganglion cells are classified as either A-cells or B-cells (Andres, 1961;Lieberman, 1971Lieberman, , 1974Duce and Keen, 1977;Rambourg et al, 1983;Willis and Coggeshall, 1991;Lawson, 1992). In the light microscope, A-cells have a light cytoplasm and Nissl substance in clumps evenly distributed throughout the cell body (Fig.…”

Section: Methodsmentioning

confidence: 99%

Delayed loss of small dorsal root ganglion cells after transection of the rat sciatic nerve

2000

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Section: Methodsmentioning

confidence: 99%

Delayed loss of small dorsal root ganglion cells after transection of the rat sciatic nerve

2000

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“…The highest concentration of gold/silver particles (i.e., density of ␦OR immunostaining) was observed over type A 2 neurons, characterized as clear, large-diameter neurons in which evenly distributed Nissl bodies are separated from each other by pale wide strands of cytoplasm containing small stacks of Golgi saccules and rod-like mitochondria (Duce and Keen, 1977;Rambourg et al, 1983) (Fig. 4 A).…”

Section: Electron Microscopymentioning

confidence: 99%

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Gendron¹,

Lucido²,

Mennicken³

et al. 2006

J. Neurosci.

140

157

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The present study demonstrates that perikaryal ␦-opioid receptors (␦ORs) in rat dorsal root ganglion (DRG) neurons bind and internalize opioid ligands circulating in the CSF. Using confocal and electron microscopy, we found that prolonged morphine treatment increased the cell surface density of these perikaryal ␦ORs and, by way of consequence, receptor-mediated internalization of the fluorescent deltorphin (DLT) analog -Bodipy 576/589 deltorphin-I 5-aminopentylamide (Fluo-DLT) in all three types of DRG neurons (small, medium, and large). In contrast, chronic inflammatory pain induced by the injection of complete Freund's adjuvant (CFA) into one hindpaw selectively increased Fluo-DLT internalization in small and medium-sized DRG neurons ipsilateral to the inflammation. Based on our previous studies in the spinal cord of -opioid receptor (OR) knock-out mice, it may be assumed that the enhanced membrane recruitment of ␦ORs observed after sustained morphine is attributable to stimulation of ORs. However, the selectivity of the effect induced by inflammatory pain suggests that it involves a different mechanism, namely a modality-specific and pain-related activation of C and A␦ fibers. Indeed, stimulation by capsaicin of transient receptor potential vanilloid 1 receptors, which are selectively expressed by small diameter (Ͻ 600 m 2 ) DRG neurons, increased Fluo-DLT internalization exclusively in this cell population. The present results, therefore, demonstrate that DRG neurons express perikaryal ␦ORs accessible to CSF-circulating ligands and that the density and, hence, presumably also the responsiveness, of these receptors may be modulated by both pain-related stimuli and sustained exposure to OR agonists.

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“…Les neurones de type B sont plus petits, le réticulum granulaire y est réparti plus uniformément et les riboso mes libres sont très nombeux. Une analyse plus détaillée de leurs caractéristiques cyto logiques permet de distinguer plusieurs va riétés dans ces deux catégories, leurs fonc tions sont encore discutées [Andres, 1961;Duce et Keen, 1977;Jacobs et al. 1975;Pannese, I960].…”

Section: Résultatsunclassified

Etude ultrastructurale des neurones ganglionnaires spinaux au cours du vieillissement chez le rat

Aguilar

Vanneste²

1981

Cells Tissues Organs

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The chronology of the alterations impairing the cytology of the spinal ganglion neurons was analysed in 3- to 32-month-old male rats. A type neurons are impaired earlier and more severely than B neurons. Ageing alterations occur during two successive periods. During the first period, beginning at about 3 months, lipofuscin pigments gradually accumulate in the neurons. During the second period, beginning at about 24 months, various kinds of inclusions appear (granulofilamentous bodies, nematosomes,...); some of which could be originating from altered microtubules and/or filaments. At the same time, lipofuscin pigments are more heterogeneous. This period might be the onset of real senescence and of the gradual deterioration of the neuronal function.

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An ultrastructural classification of the neuronal cell bodies of the rat dorsal root ganglion using zinc iodide-osmium impregnation

Cited by 128 publications

References 14 publications

Delayed loss of small dorsal root ganglion cells after transection of the rat sciatic nerve

Delayed loss of small dorsal root ganglion cells after transection of the rat sciatic nerve

Morphine and Pain-Related Stimuli Enhance Cell Surface Availability of Somatic δ-Opioid Receptors in Rat Dorsal Root Ganglia

Etude ultrastructurale des neurones ganglionnaires spinaux au cours du vieillissement chez le rat

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