An Ultrastructural Examination of Lead Localisation in Germinating Seeds of Raphanus sativus

Lane, S.D.; Eisenacher, Martin

doi:10.1016/s0044-328x(11)80006-1

Cited by 34 publications

(8 citation statements)

References 15 publications

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“…As can be seen from the results of these studies, the situation is different in the case of lead since the greatest loss is during postfixation with osmium tetroxide. Lane & Martin (1982) also found a decrease in the number of lead deposits after osmium tetroxide was used. They compared by EM the distribution of lead in Raphanus sativus embryos after fixation in glutaraldehyde only and in glutaraldehyde + osmium tetroxide.…”

Section: Discussionmentioning

confidence: 88%

“…Electron microscopy (EM) techniques are very useful in localizing lead in plant tissues (e.g. Richter et al ., 1968 ; Malone et al ., 1974 ; Sharpe & Denny, 1976; Lane & Martin, 1982; Ksia¸żek et al ., 1984 ; Hübner et al ., 1985 ; Wierzbicka, 1987a, b). They make it possible to identify the main accumulations of lead in cells and cellular organelles, which is very helpful in elucidating the mechanisms of plant tolerance to lead (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Localization of lead in Allium cepa L. cells by electron microscopy

Antosiewicz

Wierzbicka

1999

Journal of Microscopy

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The study of mechanisms by which plants tolerate lead requires ultrastructural observations of lead distribution in cells. However, the conventional technique used in electron microscopy brings up the problem of lead translocation from tissues during chemical processing. If most of the lead is washed out of tissues, then the method is not suitable for this type of study; if, however, it remains in the tissues, the method can be used. The amount of lead washed out of Allium cepa root tips during successive stages of fixing and dehydrating was determined in this study. Allium cepa plants were treated with Pb(NO3)2 in hydroponic cultures. The conventional fixing (GA, OsO4) and dehydration (alcohol + propylene oxide or acetone) methods used in the preparation of tissues for electron microscopy were then applied to root tips. The lead content in tissues and in reagents was analysed in the successive stages of the procedure. It was shown that 96.2% of the lead taken up was retained in the tissues and was not washed out during fixing and dehydrating. Of the 3.8% of the lead lost in the chemical procedure, about half was washed out during fixing in osmium tetroxide. This reagent is thus the least suitable for this type of study. Our study showed that the conventional electron microscopy preparative technique is appropriate for studying the distribution of lead deposits in A. cepa root cells, owing to the way in which lead is compartmentalized/sequestered in plant cells.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Localization of lead in Allium cepa L. cells by electron microscopy

Antosiewicz

Wierzbicka

1999

Journal of Microscopy

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“…In experiments on lead influence on different plant species, conducted by other autbors, necroses were also a frequently mentioned symptom of the harmful influence of this element. It is reported, among others, by LANE and MARTIN (1980) who conducted research on the influence of lead on Raphanus sativus. Necroses were also observed by WOLDU (1981) and ZATORSKA (1981) on isolated cotyledons oi Lupinus luteus, and KRUPINSKA (1981) in the Bryophytes.…”

Section: Resultsmentioning

confidence: 88%

The sensitivity of poplar leaves to lead nitrate and the intracellular localization of lead¹

Ksiazek¹,

Woźny²,

Siwecki

1984

European Journal of Forest Pathology

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The sensitivity to lead nitrate of four chosen poplar speeies has been investigated. P. maximowiezii turned out to he the most sensitive, and P. nigra the least sensitive one. Morphologically lead presence in the leaves manifested itself with a ehange of colour (from green to more or less reddish-brown) of the leaf venation and petioles, and with necrosis of some parts of the blade. At the ultrastructural level lead deposits were present m.iinly within the vascular elements of the leaves.

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“…Indeed, after growing plants in the presence of Cd and Pb, these metals were abundant in cell walls of many dicotyledonous plant species [24,26,42,70,77,78]. Indeed, after growing plants in the presence of Cd and Pb, these metals were abundant in cell walls of many dicotyledonous plant species [24,26,42,70,77,78].…”

Section: Primary Cortexmentioning

confidence: 99%

10.1007/s11183-008-1001-8

2010

CrossRef Listing of Deleted DOIs

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The review considers the roles of root and shoot tissues in transport and accumulation of heavy metals in plants of two contrast groups, i.e., excluders and hyperaccumulators. The regularities in distribution of cadmium, lead, nickel, and strontium are summarized. Effects of other cations, calcium in particular, on accumulation and distribution of heavy metals are analyzed. Specific patterns of metal distribution in hyperaccumulator plants are discussed together with morphological and functional features underlying the ability of plants to accumulate heavy metals in the aboveground organs. Based on the data available, the root and shoot tissues are classified according to their roles in transport and distribution of the metals examined.

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An Ultrastructural Examination of Lead Localisation in Germinating Seeds of Raphanus sativus

Cited by 34 publications

References 15 publications

Localization of lead in Allium cepa L. cells by electron microscopy

Localization of lead in Allium cepa L. cells by electron microscopy

The sensitivity of poplar leaves to lead nitrate and the intracellular localization of lead¹

10.1007/s11183-008-1001-8

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An Ultrastructural Examination of Lead Localisation in Germinating Seeds of Raphanus sativus

Cited by 34 publications

References 15 publications

Localization of lead in Allium cepa L. cells by electron microscopy

Localization of lead in Allium cepa L. cells by electron microscopy

The sensitivity of poplar leaves to lead nitrate and the intracellular localization of lead1

10.1007/s11183-008-1001-8

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The sensitivity of poplar leaves to lead nitrate and the intracellular localization of lead¹