2015
DOI: 10.1038/srep09127
|View full text |Cite
|
Sign up to set email alerts
|

An Unprecedented alteration in mode of action of IsCT resulting its translocation into bacterial cytoplasm and inhibition of macromolecular syntheses

Abstract: IsCT, a 13-residue, non-cell-selective antimicrobial peptide is comprised of mostly hydrophobic residues and lesser cationic residues. Assuming that placement of an additional positive charge in the non-polar face of IsCT could reduce its hydrophobic interaction, resulting in its reduction of cytotoxicity, an analog, I9K-IsCT was designed. Two more analogs, namely, E7K-IsCT and E7K,I9K-IsCT, were designed to investigate the impact of positive charges in the polar face as well as polar and non-polar faces at a … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

1
30
0

Year Published

2016
2016
2024
2024

Publication Types

Select...
7

Relationship

1
6

Authors

Journals

citations
Cited by 26 publications
(31 citation statements)
references
References 59 publications
1
30
0
Order By: Relevance
“…The morphological changes to E. coli induced by the piscidin-1-derived peptides were studied using scanning electron microscopy (SEM), as described earlier (22,27). Micrographs were taken at magnifications of ϫ24,000.…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…The morphological changes to E. coli induced by the piscidin-1-derived peptides were studied using scanning electron microscopy (SEM), as described earlier (22,27). Micrographs were taken at magnifications of ϫ24,000.…”
Section: Methodsmentioning
confidence: 99%
“…The peptide-induced depolarization of the lipid bilayer was measured by determining the ability of the peptides to dissipate the diffusion potential across lipid vesicles composed of zwitterionic PC-Chol (8:1, wt/wt) or negatively charged PC-PG (3:1 wt/wt) by employing a potential-sensitive dye, diS-C 3 -5, as described in earlier reports (16,22).…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…These compounds have the mechanism of action of other amphipathic peptides. However, in a recent paper, Ghosh also suggests that some IsCT analogs may have activity without disrupting the cell membrane by penetrating it and inhibiting bacterial macromolecule biosynthesis, such as nucleic acids and proteins [ 15 ].…”
Section: Introductionmentioning
confidence: 99%