An unspecific phytotoxin oxalic acid and its effect on sunflower proteome

Abstract

Oxalic acid (OA) is found naturally in many plants and animals: it plays diverse roles in nature. It is an important pathogenicity determinant of many necrotrophic pathogens including Sclerotinia sclerotiorum (Lib.) de Bary. In order to understand the resistance mechanisms in Helianthus annuus L., a proteomic study was conducted on sunflower 12 h after inoculation by OA. A total of 17 differentially expressed proteins (either OA-induced or -suppressed proteins) were identified as a result of OA treatment. The candidate proteins were classified into two groups depending on their up/downregulation. The first group – upregulated proteins – included 13 proteins identified as being involved in the Calvin cycle, photosynthesis, programmed cell death (PCD) pathway, heat shock proteins, proteins with antioxidant activities and flavonol synthase (FLS). The second group – downregulated proteins – included those from the cupin family, ATP synthase subunit β, ketol-acid reducto-isomerase, and actin. Studying the biological significance of proteins responsive to OA might ultimately convey us to improve sunflower lines with higher levels of resistance to Sclerotinia and help to control this devastating necrotrophic plant pathogen.

Suppression of clubroot (Plasmodiophora brassicae) development in Brassica campestris sp. chinensis L. via exogenous inoculation of Piriformospora indica

“…Flavonols, such as quercetin have been reported to provide resistance to pathogen attack by suppressing ATPase activity of DNA gyrase (Babii et al, 2016;Naoumkina et al, 2010;Plaper et al, 2003). Similarly, kaempferide (flavonol triglycoside) produced by Dianthus caryophyllus is a fungitoxic compound against Fusarium oxysporum (Curir, Dolci, & Galeotti, 2005;Monazzah, Enferadi, Soleimani, & Rabiei, 2016). Flavonoids may be involved in the auxin transportation to the infected root by P. brassicae for gall formation (Paesold, Siegel, Seidel, & Ludwig-Müller, 2010).…”

Enzymatic activities and pathogenesis-related genes expression in sunflower inbred lines affected bySclerotinia sclerotiorumculture filtrate

“…Since OA can regulate antioxidant system in infected plants (Williams et al 2011), it is important to compare the antioxidant system of lines with different resistance levels to S. sclerotiorum infection in order to better understand the resistance mechanisms towards S. sclerotiorum in sunflower. The previous studies also indicated the increased activity of shikimate dehydrogenase (SKDH) and protein abundance of flavonol synthase in sunflower plants treated by OA which might implicate the induction of phenylpropanoid pathway (Tahmasebi-Enferadi et al 2011;Monazzah et al 2016).…”

Extraction and identification of biologically important proteins from the medicinal plant God’s crown ( Phaleria macrocarpa )

“…So, the protein characterizations could lead to the discovery of new bioactive molecules hence increases the medicinal values of P. macrocarpa (Capriotti et al., 2013). It was also suggested that the different represented proteins in plants could generate knowledge about cell signaling pathways, providing supplementary informtion and also, it is essential in understanding the proteome changes of the plant in response to pathogens (Cipriano et al., 2015; Monazzah et al., 2016).…”