An unusual and atypical presentation of the novel coronavirus: A case report and brief review of the literature

Alshoabi, Sultan Abdulwadoud; Haider, Kamel H.; Mostafa, Mahmoud A; Hamid, Abdullgabbar M.; Daqqaq, Tareef S

doi:10.1016/j.jtumed.2021.01.014

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…A potential impact of COVID-19 infection on the pampiniform plexus might be suggested by several case reports of COVID-19 associated thrombosis located in the pampiniform plexus [62][63][64][65] . Additionally, the signal distribution of the left and right testes is distinct, with the signal of the right testicle being more focused at the top of the testicle while the signal on the left testicle being more distributed in the spermatic cord.…”

Section: Discussionmentioning

confidence: 99%

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

Madden

Thomas

Blair

et al. 2022

Preprint

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Because of the recognized systemic nature of SARS-CoV-2 infection, a non-invasive and unbiased method is needed to clarify its spatiotemporal dynamics in vivo after transmission. We recently developed a probe based on the anti-SARS-CoV-2 spike antibody CR3022 to study SARS-CoV-2 pathogenesis in vivo. Herein, we describe its use in immunoPET to investigate SARS-CoV-2 infection of rhesus macaques. Using PET/CT imaging of macaques at different times post-SARS-CoV-2 inoculation, we demonstrate that the 64Cu-labelled CR3022-F(ab′)2 probe targeting the spike protein of SARS-CoV-2 can be used to study the dynamics of infection within the respiratory tract and uncover novel sites of infection. Differences in lung pathology between infection with the WA1 isolate and the delta variant were readily observed using immunoPET and corroborated CT lung pathology. The ability of the probe to illuminate lung-associated pathology demonstrates its specificity and function to detect infection in PET scan. The 64Cu-CR3022-probe also demonstrated dynamic changes occurring between 1- and 2-weeks post-infection. Remarkably, a robust signal was seen in the male genital tract (MGT) of all three animals studied. Infection of the MGT was validated by immunofluorescence imaging of infected cells in the testicular and penile tissue and severe pathology was observed in the testes of one animal at 2-weeks post-infection. The results presented here underscore the utility of using immunoPET to study the dynamics of SARS-CoV-2 infection to understand its pathogenicity and discover new anatomical sites of viral replication. We provide direct evidence for SARS-CoV-2 infection of the MGT in rhesus macaques revealing the possible pathologic outcomes of viral replication at these sites.

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Section: Discussionmentioning

confidence: 99%

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

Madden

Thomas

Blair

et al. 2022

Preprint

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show abstract

“…The outbreak of SARS-CoV-2 virus infection in 2019 has resulted in a global pandemic of Coronavirus Disease 2019 (COVID-19) [ 1 ]. Virus invasion elicits an immune response, which frequently results in fever [ 2 , 3 , 4 ]. This process greatly speeds up the adhesion and migration of immune cells to lymph nodes and tissues at the site of infection.…”

Section: Introductionmentioning

confidence: 99%

Factors Affecting the Antibody Immunogenicity of Vaccines against SARS-CoV-2: A Focused Review

et al. 2021

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Vaccines are a crucial part of the global anti-pandemic effort against COVID-19. The effects of vaccines, as well as their common influencing factors, are the most important issues that we should focus on at this time. To this end, we review statistics on immunogenicity after vaccination, using neutralizing antibodies as the main reference index. Age, infection history, and virus variants are compared, and vaccination program recommendations are made accordingly. Suggestions are made to address concerns raised by the vaccines’ shortened development cycle, as well as the emergence of immunity escape of viral variants. Finally, a brief description and future prospects are provided based on the principle of the ADE effect and previous experience with similar viruses.

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An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

Madden¹,

Thomas²,

Blair³

et al. 2022

Preprint

View full text Add to dashboard Cite

The systemic nature of SARS-CoV-2 infection is highly recognized, but poorly characterized. A non-invasive and unbiased method is needed to clarify whole body spatiotemporal dynamics of SARS-CoV-2 infection after transmission. We recently developed a probe based on the anti-SARS-CoV-2 spike antibody CR3022 to study SARS-CoV-2 pathogenesis in vivo. Herein, we describe its use in immunoPET to investigate SARS-CoV-2 infection of three rhesus macaques. Using PET/CT imaging of macaques at different times post-SARS-CoV-2 inoculation, we track the 64Cu-labelled CR3022-F(ab’)2 probe targeting the spike protein of SARS-CoV-2 to study the dynamics of infection within the respiratory tract and uncover novel sites of infection. Using this method, we uncovered differences in lung pathology between infection with the WA1 isolate and the delta variant, which were readily corroborated through computed tomography scans. The 64Cu-CR3022-probe also demonstrated dynamic changes occurring between 1- and 2-weeks post-infection. Remarkably, a robust signal was seen in the male genital tract (MGT) of all three animals studied. Infection of the MGT was validated by immunofluorescence imaging of infected cells in the testicular and penile tissue and severe pathology was observed in the testes of one animal at 2-weeks post-infection. The results presented here underscore the utility of using immunoPET to study the dynamics of SARS-CoV-2 infection to understand its pathogenicity and discover new anatomical sites of viral replication. We provide direct evidence for SARS-CoV-2 infection of the MGT in rhesus macaques revealing the possible pathologic outcomes of viral replication at these sites.

show abstract

An unusual and atypical presentation of the novel coronavirus: A case report and brief review of the literature

Cited by 4 publications

References 21 publications

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

Factors Affecting the Antibody Immunogenicity of Vaccines against SARS-CoV-2: A Focused Review

An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques

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