It has been recognized that disturbance is an important factor affecting community composition and biodiversity in natural ecosystems (Brunbjerg et al., 2015). Agriculture, urbanization, and tourism are disturbances that considerably alter local landscapes and climates and are increasing in intensity, constituting some of the greatest threats to ecosystems (Brooks et al., 2006). These threats often lead to a reduction in vegetation cover, modifications in habitat complexity, heat retention, and landscape connectivity (Bierwagen, 2007; Cadenasso et al., 2007; Yuan and Bauer, 2007). Moreover, they have an important effect on biodiversity, driving major changes in species abundances and distributions (McKinney, 2008). The Ventania hill system is a unique upland ecosystem in central Argentina. In addition, it is one of the last relicts of relatively well-conserved areas of Pampean grassland where several endemic taxa and unique habitat types can be found (Zalba and Cozzani, 2004; Delucchi, 2006). Unfortunately, this hill system shows many ecosystem disturbances: overgrazing by large and exotic herbivores including feral horses and livestock (Loydi and Distel, 2010; Scorolli and López-Cazorla, 2010); invasion of pine and other exotic plants (Zalba and Villamil, 2002; de Villalobos et al., 2011); tourism and urbanization (Gil et al., 2014). It has been demonstrated that spiders are good candidates for studies related to human impact and conservation, and may be useful as biological indicators for particular ecosystems (Scott et al., 2006; Ghione et al., 2013; Schwerdt et al., 2017). Arguably, knowing the density and distributional patterns of a common spider species could be very important in elucidating the potential of the species as a biological indicator for assessing the conservation degree of native grassland. Nemesiidae spiders are widely distributed across the tropical and subtropical regions of South America, but data about their biology and ecology is scarce, with only notes and some particular ecological studies available from a few species from