An update and review of narrowband ultraviolet B phototherapy for vitiligo

Wang, Eugene; Rodrigues, Michelle

doi:10.1002/der2.142

Cited by 5 publications

(2 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The oxidative modification of HSA was assessed based on the decrease in fluorescence in the region of 350 nm (fluorescence of tryptophan and tyrosine) upon excitation at 280 nm. The light with the spectral range of 308 ± 10 nm, used in the treatment of vitiligo [18,42,43], was considered as the source of oxidative stress. Figure 8a shows the HSA fluorescence spectra before and after irradiation at 308 nm for 16 min.…”

Section: Study Of Hsa Oxidative Modification With Fluorescencementioning

confidence: 99%

Tetrahydrobiopterin as a Trigger for Vitiligo: Phototransformation during UV Irradiation

Telegina,

Vechtomova,

Borzova

et al. 2023

IJMS

View full text Add to dashboard Cite

Vitiligo is a type of hypomelanosis. Tetrahydrobiopterin (H4Bip), the coenzyme of the initial stage of melanogenesis, appears to be a trigger for vitiligo. H4Bip is present in vitiligo in 3–5-fold excess and causes oxidative stress by triggering an autocatalytic cycle of excess hydrogen peroxide synthesis. Using quantum-chemical calculations, we have evaluated the possibility of H4Bip reactions occurring in the dark and under ultraviolet (UV) irradiation, including the formation of dihydropterin dimers. In order to simulate the oxidative stress, oxidative modification of human serum albumin (HSA) has been carried out in the presence of excessive H4Bip using the fluorescence method. The fraction of oxidized protein (FOP) has been calculated. It has been established that there is a strong oxidative modification of amino acids chromophores (tryptophan and tyrosine) in the protein (FOP 0.64). Under UV irradiation of the system (HSA + H4Bip), FOP is reduced to 0.39. Apparently, a part of H4Bip transforms into dihydropterin dimers and does not participate in the oxidative modification of the protein. The data on oxidative modification of HSA are consistent with dynamic light scattering: H4Bip promotes HSA aggregation with the formation of particles with a hydrodynamic radius Rh ≥ 2000 nm, which can become immunogenic.

show abstract

Section: Study Of Hsa Oxidative Modification With Fluorescencementioning

confidence: 99%

Tetrahydrobiopterin as a Trigger for Vitiligo: Phototransformation during UV Irradiation

Telegina,

Vechtomova,

Borzova

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…[76]; [132]; [133] ; [134]; [135] [136 ]; [137]; [138]; [1 39]; [140] Focused on PUVA: [141]; [142]; [143]…”

Section: Vitiligomentioning

confidence: 99%

Clinical Applications of Polypodium leucotomos (Fernblock®): An Update

Rodríguez-Luna¹,

Zamarrón²,

Juarranz³

et al. 2023

Preprint

View full text Add to dashboard Cite

Exposure to sun radiation leads to higher risk of sunburn, pigmentation, immunosuppression, photoaging and skin cancer. In addition to ultraviolet radiation (UVR), recent research indicates that infrared radiation (IR) and visible light (VIS) can play an important role in the pathogenesis of some of these processes. Detrimental effects associated to sun exposure are well known, but new studies have shown that DNA damage continues to occur long after exposure to solar radiation has ended. Regarding photoprotection strategies, natural substances are emerging for topical and oral photoprotection. In this sense, Fernblock®, a standardized aqueous extract of the fern Polypodium Leucotomos (PLE), has been widely administered both topically and orally with a strong safety profile. Thus, this extract has been used extensively in clinical practice, including as a complement to photodynamic therapy (PDT) for treating actinic keratoses (AKs) and field cancerization. It has also been used to treat skin diseases such as photodermatoses, photoaggravated inflammatory conditions and pigmentary disorders. This review examines the most recent developments in the clinical application of Fernblock® and assesses how newly investigated action mechanisms may influence its clinical use.

show abstract

Comparative Characteristics of Skin Recovery in Rats After Ultraviolet Irradiation With and Without Erbium Laser

Sulym,

Lyndin,

Shamrai

et al. 2024

EUMJ

View full text Add to dashboard Cite

Introduction. Ultraviolet radiation, which has a significant biological impact among the spectrum of sunlight, can cause skin aging and tumors, but in controlled use it also has therapeutic effects on the skin. Ultraviolet radiation with a wavelength of 311 nm is used for phototherapy of skin diseases. Melanin, contained in melanosomes (MS), is produced in melanocytes (MC) during melanogenesis. Phototherapy can cause clinical hyperpigmentation by provoking an increase in the number of MCs. Ablative lasers, such as Er:YAG, are used to manage hyperpigmentation, but at the same time have the risk of causing hyperpigmentation. The frequency of such side effects varies from 10 to 46%. The aim of the study was to determine changes in the number of MCs in the epidermis relative to the length of the basement membrane and the density of MSs in the epidermis in two groups of animals: after UV irradiation without and with an ablative laser. Materials and methods. The study began with an imitation of phototherapy of dermatoses with narrow-band UV radiation. The animals were divided into 2 experimental groups: free readaptation, using laser and a separate control group (intact rats). Skin samples were taken from 54 rats: 12 animals per checkpoint (6 for each group) on days 31, 45, 61, and 121, and 6 animals in the control group. The melanocytic and melanosomal components of the epidermis were evaluated, and comparisons were made between groups and with intact animals. Results. In the group of free readaptation on day 45, the density of MCs reached 0.78±0.16 cells per 100 mkm of basal membrane (units), which exceeded the normal value, but did not show statistical significance (p=0.37). On days 61 and 121, the density was 0.72±0.13 units and 0.70±0.17 units, respectively, which indicated a correlation with the elapsed time, r = -0.732, but at once was not significantly different from the control group. In the laser hypopigmentation group, the density of MCs on the basement membrane was 0.68±0.26, 0.70±0.26 and 0.69±0.23 units on days 45, 61 and 121, respectively, demonstrating stability. The correlation between MCs density and time after UV irradiation was not taken into account (r = ‑0.485). As for the analysis of MSs, in the control group, the area of MSs was 0.77±0.24% of the epidermis square. On day 31, the density of MSs reached a peak and amounted to 1.21±0.32%, statistically different from the control group (p=0.04). Subsequently, in the group of free readaptation, the area of MSs decreased to 1.03±0.16% on day 45, 0.91±0.13% on day 61, and 0.91±0.25% on day 121. An inverse correlation was observed between the density of MSs and the elapsed time, r = -0.735. Similar patterns were observed in the laser ablation group. On days 45, 61, and 121, the area of MSs was 1.07±0.28%, 0.92±0.22%, and 0.84±0.19%, respectively. The correlation between MSs density and time after UV irradiation was significant, r = -0.881. Comparison of the density of MSs in the epidermis between the experimental groups did not reveal statistical differences at any stage of the experiment. Conclusions. The described ultraviolet impact on the skin did not lead to a statistically significant increase in the number of MCs on the basal membrane (p=0.37), but significantly increased the area of MSs in the epidermis (p=0.04). Comparison of the density of MCs in the groups of laser hypopigmentation and free readaptation did not reveal statistically significant differences between each other and the control group. Laser ablation did not lead to either an increase or decrease in the average concentration of MSs in the epidermis compared to the free readaptation group.

show abstract

An update and review of narrowband ultraviolet B phototherapy for vitiligo

Cited by 5 publications

References 86 publications

Tetrahydrobiopterin as a Trigger for Vitiligo: Phototransformation during UV Irradiation

Tetrahydrobiopterin as a Trigger for Vitiligo: Phototransformation during UV Irradiation

Clinical Applications of Polypodium leucotomos (Fernblock®): An Update

Comparative Characteristics of Skin Recovery in Rats After Ultraviolet Irradiation With and Without Erbium Laser

Contact Info

Product

Resources

About