An update on faecal carriage of ESBL-producing Enterobacteriaceae by Portuguese healthy humans: detection of the<i>H</i>30 subclone of B2-ST131<i>Escherichia coli</i>producing CTX-M-27: Table 1.

Rodrigues, Carla; Machado, Elisabete; Fernandes, Sofia; Peixe, Luı́sa; Novais, Ângela

doi:10.1093/jac/dkv443

Cited by 37 publications

(37 citation statements)

References 12 publications

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“…ST131 containing bla CTX-M-27 responsible for human infections has been reported from various continents ( 2 ) and is especially common among ESBL-producing ExPEC in certain countries, such as Israel, the Czech Republic, and Switzerland ( 2 , 13 , 14 ). CTX-M-27–producing ST131 also is present among nonclinical and nonhuman E. coli isolates, including fecal specimens of healthy children attending day care centers in France; fecal specimens of healthy adults in China, Portugal, and the Netherlands; samples from sick dogs and cats in Japan; samples from water birds from central Europe and Swiss rivers and lakes; and samples of well water from China ( 2 , 10 , 11 , 15 , 23 – 25 ). The most common ESBL among E. coli ST131 in nonhuman samples is CTX-M-27 ( 2 , 23 – 25 ).…”

Section: Discussionmentioning

confidence: 99%

GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

Matsumura¹,

Pitout²,

Gomi³

et al. 2016

Emerg. Infect. Dis.

158

138

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Section: Discussionmentioning

confidence: 99%

GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

Matsumura¹,

Pitout²,

Gomi³

et al. 2016

Emerg. Infect. Dis.

158

138

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“…before but have been identified among different genera. B la SHV -11 have been found in many clinical and environmental K. pneumoniae strains (Alibi et al, 2015; Davies et al, 2016; Shahraki-Zahedani et al, 2016), bla CTX-M-27 in many Enterobacteriaceae strains (Matsumura et al, 2016; Rodrigues et al, 2016), bla CTX-M-98 in E. coli strains from China (Liu et al, 2015) and bla PER-4 in Proteus vulgaris clinical strain (NG_049963). The rest of the identified ESBL bla genes have been observed in Aeromonas spp.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Variety of β-Lactamase Genes among Aeromonas spp. Isolated from Urban Wastewater Treatment Plant

et al. 2017

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Members of the genus Aeromonas that commonly occur in various aquatic ecosystems are taken into account as vectors spreading antibiotic resistance genes (ARGs) in the environment. In our study strains of Aeromonas spp. (n = 104) not susceptible to ampicillin were isolated from municipal sewage of different levels of purification – raw sewage, activated sludge and treated wastewater. The crucial step of the study was the identification of β-lactamase resistance genes. The identified genes encode β-lactamases from 14 families – blaTEM, blaOXA, blaSHV, blaCTX-M, blaMOX, blaACC, blaFOX, blaGES, blaPER, blaV EB, blaKPC, cphA, imiH, and cepH. There were no significant differences in number of identified ARGs between isolation points. BlaOXA, blaFOX variants and, characteristic for Aeromonas genus, metallo-β-lactamase cphA-related genes were the most commonly identified types of β-lactam resistance determinants. Moreover, we found four extended-spectrum β-lactamases (blaSHV -11, blaCTX-M-27, blaCTX-M-98, and blaPER-4) – and seven AmpC (blaACC, blaFOX-2-like, blaFOX-3, blaFOX-4-like, blaFOX-9, blaFOX-10-like, and blaFOX-13-like) types and variants of genes that had never been found among Aeromonas spp. before. Five of the β-lactamases families (blaTEM, blaOXA, blaFOX, blaV EB, and cphA) were identified in all three isolation sites, which supports the hypothesis that wastewater treatment plants (WWTPs) are hot spots of ARGs dissemination. The obtained ARGs sequences share high identity with previously described β-lactamases, but new variants of those genes have to be considered as well. Characterization of antibiotic susceptibility was performed using disk the diffusion method with 12 different antibiotics according to CLSI guidelines. Over 60% of the strains are unsusceptible to cefepime and chloramphenicol and the majority of the strains have a multidrug resistance phenotype (68%). Finally, analysis of plasmid profiles among the resistant strains showed that 62% of the isolates from all three points of the WWTP carry plasmids of different sizes. Among some of the isolated plasmids blaFOX-4-like and blaGES genes have been found. To sum up, the results strongly suggest that Aeromonas spp. can be considered as agents of antibiotic resistance dissemination from wastewater to the natural environment.

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“…Compared to bla CTX-M-14 , bla CTX-M-27 has a decreased K m for ceftazidime, producing a corresponding increase in MIC (53). F-group plasmids with bla CTX-M-27 alleles are much more common in E. coli isolates (94), and bla CTX-M-27 is now found frequently in the ST131 group of E. coli (74,76,95,96), although these studies also note that the context of the loci are limited or infrequently reported.…”

Section: Discussionmentioning

confidence: 91%

“…We can envisage enteric ST882S encountering an ESBL donor in the gut milieu, followed by a secondary invasive infection of ST931R during antibiotic treatment. Carriage by healthy (72) and gastrointestinal-symptomatic (73) adults of ESBL-positive E. coli and bla CTX-M-27 in particular (74) has been documented, although specific plasmids were not identified. Unfortunately, the diagnostic regime for our patient did not require stool cultures, which may in retrospect have been used to identify an ESBL donor organism.…”

Section: Discussionmentioning

confidence: 99%

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

McCollister

Kotter

Frank

et al. 2016

Antimicrob Agents Chemother

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bWe report a case of ceftriaxone treatment failure for bacteremia caused by Salmonella enterica subsp. enterica serovar Typhimurium, due to the in vivo acquisition of a bla CTX-M-27 -encoding IncFII group transmissible plasmid. The original ␤-lactamasesusceptible isolate ST882S was replaced by the resistant isolate ST931R during ceftriaxone treatment. After relapse, treatment was changed to ciprofloxacin, and the patient recovered. Isolate ST931R could transfer resistance to Escherichia coli at 37°C. We used whole-genome sequencing of ST882S and ST931R, the E. coli transconjugant, and isolated plasmid DNA to unequivocally show that ST882S and ST931R had identical chromosomes, both having 206 identical single-nucleotide polymorphisms (SNPs) versus S. Typhimurium 14028s. We assembled a complete circular genome for ST931R, to which ST882S reads mapped with no SNPs. ST882S and ST931R were isogenic except for the presence of three additional plasmids in ST931R. ST931R and the E. coli transconjugant were ceftriaxone resistant due to the presence of a 60.5-kb IS26-flanked, bla CTX-M-27 -encoding IncFII plasmid. Compared to 14082s, ST931R has almost identical Gifsy-1, Gifsy-2, and ST64B prophages, lacks Gifsy-3, and instead carries a unique Fels-2 prophage related to that found in LT2. ST882S and ST931R both had a 94-kb virulence plasmid showing >99% identity with pSLT14028s and a cryptic 3,904-bp replicon; ST931R also has cryptic 93-kb IncI1 and 62-kb IncI2 group plasmids. To the best of our knowledge, in vivo acquisition of extended-spectrum ␤-lactamase resistance by S. Typhimurium and bla CTX-M-27 genes in U.S. isolates of Salmonella have not previously been reported. G reater than 1.4 million cases of nontyphoidal salmonellosis are estimated to occur each year in the United States, with approximately 95% of cases resulting from foodborne transmission (1). Although most infections result in mild to moderate gastroenteritis that usually resolves with or without treatment, concurrent bacteremia is observed in approximately 6% of patients and can be associated with serious disease, especially in infants aged Ͻ1 year, the elderly, and those with immunocompromised states such as HIV infection (2-5). Although routine use of antimicrobial therapy is not generally recommended for the treatment of most Salmonella infections, such therapy may be lifesaving in persons with invasive disease.Treatment of invasive salmonellosis has been compromised in recent years due to the emergence of Salmonella isolates with single or multidrug resistance to a number of first-line agents (6, 7). Antimicrobial resistance in strains of nontyphoidal Salmonella (NTS) has been linked to the use of antimicrobial agents in livestock (8-11). Consequently, agents such as fluoroquinolones and third generation cephalosporins, such as ceftriaxone, have become treatment modalities of choice for therapy against severe Salmonella infections. In the United States before 1996, all reported cases of infection with ceftriaxone-resistant NTS isolates were known o...

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An update on faecal carriage of ESBL-producing Enterobacteriaceae by Portuguese healthy humans: detection of theH30 subclone of B2-ST131Escherichia coliproducing CTX-M-27: Table 1.

Cited by 37 publications

References 12 publications

GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

Occurrence and Variety of β-Lactamase Genes among Aeromonas spp. Isolated from Urban Wastewater Treatment Plant

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

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An update on faecal carriage of ESBL-producing Enterobacteriaceae by Portuguese healthy humans: detection of theH30 subclone of B2-ST131Escherichia coliproducing CTX-M-27: Table 1.

Cited by 37 publications

References 12 publications

GlobalEscherichia coliSequence Type 131 Clade withblaCTX-M-27Gene

GlobalEscherichia coliSequence Type 131 Clade withblaCTX-M-27Gene

Occurrence and Variety of β-Lactamase Genes among Aeromonas spp. Isolated from Urban Wastewater Treatment Plant

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection

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GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

GlobalEscherichia coliSequence Type 131 Clade withbla_CTX-M-27Gene

Whole-Genome Sequencing Identifies In Vivo Acquisition of a bla _CTX-M-27 -Carrying IncFII Transmissible Plasmid as the Cause of Ceftriaxone Treatment Failure for an Invasive Salmonella enterica Serovar Typhimurium Infection