An Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Domestic Animals Described in 2018 to 2021

Munson, Erik; Lawhon, Sara D.; Burbick, Claire R.; Zapp, Amanda; Villaflor, Maia; Thelen, Elizabeth

doi:10.1128/jcm.00281-22

Cited by 8 publications

(2 citation statements)

References 226 publications

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“…The brucellosis infection is brought on by tiny, aerotolerant, Gram-negative infectious pathogens belonging to the Brucella family. The 12 species in the Brucella family each have unique host preferences, levels of pathogenicity, and morphological characteristics (Munson et al 2022 ; Dahouk et al 2017 ). B. melitensis , B. abortus , B. suis , B. canis , B. ovis , and B. neotomae are classic Brucella species.…”

Section: Introductionmentioning

confidence: 99%

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Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus

2023

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This work aimed to provide recombinant Lactococcus lactis as a potential live vector for the manufacture of recombinant Brucella abortus (rBLS-Usp45). The sequences of the genes were collected from the GenBank database. Using Vaxijen and ccSOL, the proteins' immunogenicity and solubility were evaluated. Mice were given oral vaccinations with recombinant L. lactis . Anti-BLS-specific IgG antibodies were measured by ELISA assay. Cytokine reactions were examined using real-time PCR and the ELISA technique. The BLS protein was chosen for immunogenicity based on the vaccinology screening findings since it had maximum solubility and antigenic values of 99% and 0.75, respectively. The BLS gene, digested at 477 bp, was electrophoretically isolated to demonstrate that the recombinant plasmid was successfully produced. Protein-level antigen expression showed that the target group produced the 18 kDa-sized BLS protein, whereas the control group did not express any proteins. In the sera of mice given the L. lactis -pNZ8148-BLS-Usp45 vaccine 14 days after priming, there was a significant level of BLS-specific IgG1, IgG2a ( P < 0.001) compared to the PBS control group. Vaccinated mice showed higher levels of IFN-γ, TNFα, IL-4, and IL-10 in samples obtained on days 14 and 28, after receiving the L. lactis -pNZ8148-BLS-Usp45 and IRBA vaccines ( P < 0.001). The inflammatory reaction caused less severe spleen injuries, alveolar edema, lymphocyte infiltration, and morphological damage in the target group's spleen sections. Based on our findings, an oral or subunit-based vaccine against brucellosis might be developed using L. lactis -pNZ8148-BLS-Usp45 as a novel, promising, and safe alternative to the live attenuated vaccines now available. Supplementary Information The online version contains supplementary material available at 10.1007/s00203-023-03471-6.

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Section: Introductionmentioning

confidence: 99%

“…According to the above content, each of the above vaccines has limitations. Therefore, there is a great need to develop a vaccine that does not have these limitations (Wyszyska et al 2015; Munson et al 2022 ; Dahouk Al et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus

2023

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Update on novel validly published taxa of bacteria isolated from domestic animals described in 2022

Lawhon,

Burbick,

Munson

et al. 2023

J Clin Microbiol

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Expansion of our knowledge of the microbial world continues to progress at a rapid rate and carries with it an associated need for recognizing and understanding the implications of those changes. Here, we describe additions of novel taxa from domestic animals published in 2022 that are validly published per the International Code of Nomenclature of Prokaryotes. These included new members of Staphylococcaceae , Moraxella nasovis sp. nov. in sheep with respiratory disease, three additions to Campylobacteraceae (including one from chickens with spotty liver disease), and multiple additions of organisms from the microbiota of dogs, pigs, and especially honeybees and other important pollinators. Noteworthy additions were associated with diseases of cattle, including mastitis, endocarditis, orchitis, and endometritis. Also described in 2022 was Pseudochrobactrum algeriense sp. nov., a member of the Brucellaceae family, isolated from the mammary lymph nodes of cows.

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An update on novel taxa and revised taxonomic status of bacteria isolated from non-domestic animals described in 2022

Burbick,

Lawhon,

Munson

et al. 2023

J Clin Microbiol

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Numbers of new and revised microbial taxa are continuously expanding, and the rapid accumulation of novel bacterial species is challenging to keep up with in the best of circumstances. With that in mind, following the template of reports on prokaryotic species isolated from humans, this is now the second publication summarizing new and revised taxa in non-domestic animal species in the Journal of Clinical Microbiology . The majority of new taxa were obtained as part of programs to identify bacteria from mucosal surfaces and the gastrointestinal tract from healthy wildlife. A few notable bacteria included new Erysipelothrix spp. from mammalian and aquatic sources and a novel Bartonella spp. isolated from a rodent, both of which could be considered members of emerging and re-emerging genera with pathogenic potential in humans and animals.

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An Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Domestic Animals Described in 2018 to 2021

Cited by 8 publications

References 226 publications

Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus

Oral vaccination with novel Lactococcus lactis mucosal live vector-secreting Brucella lumazine synthase (BLS) protein induces humoral and cellular immune protection against Brucella abortus

Update on novel validly published taxa of bacteria isolated from domestic animals described in 2022

An update on novel taxa and revised taxonomic status of bacteria isolated from non-domestic animals described in 2022

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