An update on recent developments in the prevention and treatment of Staphylococcus aureus biofilms

Suresh, Maneesha K.; Biswas, Raja; Biswas, Lalitha

doi:10.1016/j.ijmm.2018.11.002

Cited by 117 publications

(92 citation statements)

References 144 publications

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“…Multidrug efflux pumps received special attention in the past years due to their potential association with clinical multidrug resistance 83 Furthermore, recent advances demonstrate an important role of multidrug efflux pumps with biofilm formation, both mechanisms regulated by quorum-sensing mechanisms 84 . Since multidrug-resistant bacteria and biofilms have been the main targets in phage therapy 85,86 , these findings can reinforce the importance of phage study for more efficient control of S. aureus in mastitis.…”

Section: General Features Of S Aureus 3059 and Ufv2030rh1 Genomes Amentioning

confidence: 79%

Milk microbial composition of Brazilian dairy cows entering the dry period and genomic comparison between Staphylococcus aureus strains susceptible to the bacteriophage vB_SauM-UFV_DC4

Duarte

Treu

Sartori

et al. 2020

Sci Rep

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Brazil has the second-largest dairy cattle herd in the world, and bovine mastitis still can cause significant losses for dairy farmers. Despite this fact, little information is available about milk microbial composition of Brazilian dairy cows, as well as the potential use of bacteriophages in the control of S. aureus. Here, we investigated milk bacterial composition of 28 Holstein Fresian cows (109 teats), selected in the dry-off period, using 16S rRNA analysis. Furthermore, a representative S. aureus strain (UFV2030RH1) was obtained at drying-off for isolation of a bacteriophage (vB_SauM-UFV_DC4, UFV_DC4) and bacterial genomic comparison purposes. Our outcomes revealed that Staphylococcus was the third most prevalent genus and positively correlated with subclinical mastitis events. As a major finding, genomic analyses showed the presence of adhesive matrix molecules that recognize microbial surface components (MSCRAMM) in UFV2030RH1 and might indicate great biofilm formation capability. A minimum inhibitory concentration (MIC) assay showed that resistance to ampicillin was the highest among the antibiotic tested in S. aureus 3059 and UFV2030RH1, displaying values four and sixteen times greater than MIC resistance breakpoint, respectively. Together, our results suggest that Staphylococcus is highly prevalent in dairy cows at drying-off and the use of the phage UFV_DC4 as a biocontrol agent must be investigated in future studies. In the last few years, dairy activity in Brazil has undergone deep changes, focusing mainly on the improvement of the productive lifetime of dairy cows (i.e. milk production from birth to culling) and the possibility to reduce operation costs. Nowadays, Brazil is the fifth largest global milk producer and it has the second-largest dairy herd in the world, reflecting its relevance to the international dairy market 1. Overall, the cornerstones of a successful dairy farm activity are related to well-established nutritional, genetic and sanitary managements. As regards the latter, bovine mastitis is still the leading cause of economic losses for

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Section: General Features Of S Aureus 3059 and Ufv2030rh1 Genomes Amentioning

confidence: 79%

Milk microbial composition of Brazilian dairy cows entering the dry period and genomic comparison between Staphylococcus aureus strains susceptible to the bacteriophage vB_SauM-UFV_DC4

Duarte

Treu

Sartori

et al. 2020

Sci Rep

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“…The characteristic is the production of polysaccharide intercellular adhesin, in which the cells are embedded and protected against adverse environmental condition (Heilmann et al, 1996;Mack et al, 1996;Götz, 2002). Moreover, this structure is more resistant to desiccation, grazing, and antimicrobial agents than planktonic cells (Kot et al, 2018;Suresh et al, 2019). It is widely recognized that the prevention of biofilm adhesion is a way to solve the problem of biofilms (Soto et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Study Reveals Inhibition and Metabolic Dysregulation in Staphylococcus aureus Planktonic Cells and Biofilms Induced by Carnosol

Shen

Yuan

2020

Front. Microbiol.

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Staphylococcus aureus (S. aureus) is a global health threat accompanied by increasing in drug resistance. To combat this challenge, there is an urgent need to find alternative antimicrobial agents against S. aureus. This study investigated the antimicrobial efficacy of carnosol against S. aureus using an in vitro model. The effects of carnosol were determined based on the antimicrobial effects or formation and disruption of biofilms. Finally, metabolomics of S. aureus grown as planktonic cells and biofilms with carnosol treatment were analyzed using gas chromatography-mass spectrometry. The minimum inhibitory concentrations (MICs) of carnosol were 32 to 256 µg/mL against the sixteen tested S. aureus strains. Among the biofilms, we observed a reduction in bacterial motility of the S. aureus, biofilm development and preformed biofilm after carnosol treatment. Moreover, the significantly altered metabolic pathways upon carnosol treatment in S. aureus planktonic cells and biofilms were highly associated with the perturbation of glyoxylate and dicarboxylate metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, arginine biosynthesis, and aminoacyl-tRNA biosynthesis. In addition, glutathione metabolism, D-glutamine and D-glutamate metabolism were significantly changed in the biofilms. This study establishes the antibacterial and antibiofilm properties of carnosol, and will provide an alternative strategy for overcoming the drug resistance of S. aureus.

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“…Biofilms are structured multicellular communities of sessile bacterial cells embedded within a self-produced matrix that is made up of proteins, DNA, and polysaccharides, which together act as physical and physiological barriers to antimicrobial agents [ 23 ]. The establishment of bacterial biofilms proceeds in two phases: the primary attachment of bacterial cells on a biomaterial is followed by the accumulation of bacteria in multiple layers and glycocalyx formation, generating a mature biofilm [ 5 ]. Despite many improvements in the management of orthopedic-biofilm-related infections, treatment failure still remains a major clinical problem because within mature biofilms, the bacteria are resistant to both antibiotics and the host immune system [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…The long-term application of systemic antibiotics is necessary for acute and chronic orthopedic infections associated with mature biofilms, together with local wound debridement [ 5 ]. However, the clinical reality is that refractory infections are always caused by multidrug-resistant (MDR) Staphylococcus epidermidis or S. aureus , and after the clinical susceptibility of the bacterium involved to antibiotics, vancomycin is usually the treatment of choice for biofilm-associated infections [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Mature Staphylococcus Biofilms to Chinese Herbal Decoction Sanhuang Jiedu: An In Vitro Study

Zhang

Wang

Shi

et al. 2020

BioMed Research International

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Background. External socking and washing with the Chinese herbal Sanhuang Jiedu decoction (SHJD) can effectively control local limb infections with bone and implant exposure. However, the antibiofilm activities of this decoction in vitro have not yet been investigated. Therefore, the aim of this study was to examine the effects and characteristics of SHJD on the mature biofilms of multidrug-resistant staphylococci on a titanium surface. Methods. Biofilm-forming methicillin-resistant Staphylococcus epidermidis ATCC 35984 and S. aureus ATCC 43330, and non-biofilm-forming S. epidermidis ATCC 12228 were selected as the experimental strains. The mature biofilms were prepared on titanium surfaces. The five experimental groups were based on dilution concentrations (DC) of SHJD: the control group (biofilm incubated with 0.85% NaCl solution), the SHJD (DC:1/8) group (initial SHJD solution was diluted 1/8), the SHJD (DC:1/4) group, the SHJD (DC:1/2) group, and the SHJD (DC:1/1) group (initial SHJD solution). The effects of SHJD on the mature biofilms were observed with the bacterial spread plate method, crystal violet (CV) staining, scanning electron microscopy, and confocal laser scanning microscopy. Results. After culture in tryptic soy broth for 72 h, ATCC 43300 and ATCC 35984 produced mature biofilms and ATCC 12228 did not. The optical density value of ATCC 12228 was 0.11 ± 0.02 , significantly lower than that of ATCC 35984 ( 0.42 ± 0.05 ) or ATCC 43300 ( 0.41 ± 0.03 ) ( P < 0.05 ). The mature biofilms of ATCC 43300 and ATCC 35984 clearly disintegrated when incubated for 12–24 h with SHJD (DC:1/1) or SHJD (DC:1/2), showing only scattered bacterial adhesion. In the SHJD (DC:1/4) group, although many residual bacterial colonies still clustered together, presenting a biofilm structure, it was very looser than that in the SHJD (DC:1/8) group in which the biofilm was similar to that in the control group. For ATCC 12228, only colony adhesion was observed, and the number of colonies decreased as the concentration of SHJD or the culture period increased. The quantitative results for the bacterial spread plate and CV staining showed significant differences between the SHJD groups ( P < 0.05 ). Conclusion. SHJD has antibiofilm activity against multidrug-resistant Staphylococcus strains. It weakens or disrupts already-formed mature biofilms on titanium surfaces in a concentration- and incubation time-dependent manner.

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An update on recent developments in the prevention and treatment of Staphylococcus aureus biofilms

Cited by 117 publications

References 144 publications

Milk microbial composition of Brazilian dairy cows entering the dry period and genomic comparison between Staphylococcus aureus strains susceptible to the bacteriophage vB_SauM-UFV_DC4

Milk microbial composition of Brazilian dairy cows entering the dry period and genomic comparison between Staphylococcus aureus strains susceptible to the bacteriophage vB_SauM-UFV_DC4

Metabolomics Study Reveals Inhibition and Metabolic Dysregulation in Staphylococcus aureus Planktonic Cells and Biofilms Induced by Carnosol

Susceptibility of Mature Staphylococcus Biofilms to Chinese Herbal Decoction Sanhuang Jiedu: An In Vitro Study

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